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Rabbit Recombinant Monoclonal RPS3 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-RPS3 antibody [EPR7808] - BSA and Azide free (AB248249), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-RPS3 antibody [EPR7808] - BSA and Azide free (AB248249), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS3 antibody [EPR7808] - BSA and Azide free (AB248249), expandable thumbnail
  • OI-RD Scanning - Anti-RPS3 antibody [EPR7808] - BSA and Azide free (AB248249), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPFlow CytWB
Human
Tested
Expected
Not recommended
Not recommended
Tested
Mouse
Expected
Tested
Not recommended
Not recommended
Expected
Rat
Expected
Expected
Not recommended
Not recommended
Expected

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Human, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Component of the small ribosomal subunit (PubMed:23636399, PubMed:8706699). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:23636399, PubMed:8706699). Has endonuclease activity and plays a role in repair of damaged DNA (PubMed:7775413). Cleaves phosphodiester bonds of DNAs containing altered bases with broad specificity and cleaves supercoiled DNA more efficiently than relaxed DNA (PubMed:15707971). Displays high binding affinity for 7,8-dihydro-8-oxoguanine (8-oxoG), a common DNA lesion caused by reactive oxygen species (ROS) (PubMed:14706345). Has also been shown to bind with similar affinity to intact and damaged DNA (PubMed:18610840). Stimulates the N-glycosylase activity of the base excision protein OGG1 (PubMed:15518571). Enhances the uracil excision activity of UNG1 (PubMed:18973764). Also stimulates the cleavage of the phosphodiester backbone by APEX1 (PubMed:18973764). When located in the mitochondrion, reduces cellular ROS levels and mitochondrial DNA damage (PubMed:23911537). Has also been shown to negatively regulate DNA repair in cells exposed to hydrogen peroxide (PubMed:17049931). Plays a role in regulating transcription as part of the NF-kappa-B p65-p50 complex where it binds to the RELA/p65 subunit, enhances binding of the complex to DNA and promotes transcription of target genes (PubMed:18045535). Represses its own translation by binding to its cognate mRNA (PubMed:20217897). Binds to and protects TP53/p53 from MDM2-mediated ubiquitination (PubMed:19656744). Involved in spindle formation and chromosome movement during mitosis by regulating microtubule polymerization (PubMed:23131551). Involved in induction of apoptosis through its role in activation of CASP8 (PubMed:14988002). Induces neuronal apoptosis by interacting with the E2F1 transcription factor and acting synergistically with it to up-regulate pro-apoptotic proteins BCL2L11/BIM and HRK/Dp5 (PubMed:20605787). Interacts with TRADD following exposure to UV radiation and induces apoptosis by caspase-dependent JNK activation (PubMed:22510408).

Alternative names

OK/SW-cl.26, RPS3, Small ribosomal subunit protein uS3, 40S ribosomal protein S3

Recommended products

Rabbit Recombinant Monoclonal RPS3 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR7808
Purification technique
Affinity purification Protein A
Dissociation constant
1.36 x 10-10 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248249 is the carrier-free version of Anti-RPS3 antibody [EPR7808] - Ribosome Marker ab128995.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

RPS3 also known as Ribosomal Protein S3 is an important component of the 40S ribosomal subunit. It has a molecular mass of approximately 26 kDa. Functionally RPS3 plays a role in the translation process as part of the ribosome machinery assisting in the assembly of proteins by decoding messenger RNA. Expression of RPS3 is ubiquitous across various tissues suggesting a fundamental role for the protein in cellular functions.

Biological function summary

Protein synthesis relies heavily on components like RPS3 within the ribosomal complex where it interacts with other ribosomal proteins and rRNA to ensure accurate translation. RPS3 also exhibits DNA repair activities independently of its ribosomal function. It binds to DNA damaged sites adding a layer of protection to help maintain genomic stability by facilitating repair processes.

Pathways

RPS3 participates actively in the mRNA translation and DNA repair pathways. Inside the ribosome biogenesis pathway RPS3 interacts closely with other ribosomal proteins like RPS19 and RPS20 to form functional ribosomal units. In the context of DNA repair it aligns with interaction networks that overlap with the NHEJ (Non-Homologous End Joining) pathway thereby influencing genomic maintenance alongside proteins such as XRCC5.

Associated diseases and disorders

The dysfunction of RPS3 associates with neurodegenerative diseases and certain types of cancer. Aberrant levels or mutations can disrupt protein synthesis and DNA repair mechanisms potentially leading to tumorigenesis. In the context of cancer RPS3 shows interactions with oncogenes such as MYC linking it to pathway disturbances that can facilitate tumor progression. Similarly its misregulation is noticed in neurodegenerative contexts where protein synthesis and repair imbalances contribute to disease pathology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-RPS3 antibody [EPR7808] - BSA and Azide free (ab248249), expandable thumbnail

    Western blot - Anti-RPS3 antibody [EPR7808] - BSA and Azide free (ab248249)

    This data was developed using Anti-RPS3 antibody [EPR7808] - Ribosome Marker ab128995, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-RPS3 antibody [EPR7808] - Ribosome Marker (Anti-RPS3 antibody [EPR7808] - Ribosome Marker ab128995) at 1/1000 dilution

    Lane 1: HepG2 cell lysate at 10 µg

    Lane 2: PC-12 cell lysate at 10 µg

    Lane 3: Neuro-2a cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 27 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RPS3 antibody [EPR7808] - BSA and Azide free (ab248249), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RPS3 antibody [EPR7808] - BSA and Azide free (ab248249)

    This data was developed using Anti-RPS3 antibody [EPR7808] - Ribosome Marker ab128995, the same antibody clone in a different buffer formulation.Immunocytochemistry/ Immunofluorescence analysis of Neuro-2a (mouse neuroblastoma neuroblast) labeling RPS3 with Anti-RPS3 antibody [EPR7808] - Ribosome Marker ab128995 at 1/100 dilution. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Cells were fixed with 100% Methanol. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/00 was used as counterstain. Nuclei were stained blue with DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS3 antibody [EPR7808] - BSA and Azide free (ab248249), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS3 antibody [EPR7808] - BSA and Azide free (ab248249)

    This data was developed using Anti-RPS3 antibody [EPR7808] - Ribosome Marker ab128995, the same antibody clone in a different buffer formulation.Anti-RPS3 antibody [EPR7808] - Ribosome Marker ab128995, at a 1/50 dilution, staining RPS3 in Human kidney, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-RPS3 antibody [EPR7808] - BSA and Azide free (ab248249), expandable thumbnail

    OI-RD Scanning - Anti-RPS3 antibody [EPR7808] - BSA and Azide free (ab248249)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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