AB194670

Anti-RPS3A antibody - C-terminal

Name: Anti-RPS3A antibody - C-terminal (ab194670) | Abcam
Brand: Abcam Limited
SKU: AB194670
Price: 545 USD
Availability: InStock

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(3 Publications)

Mouse Polyclonal RPS3A antibody. C-terminal. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RPS3A aa 150 to C-terminus.

View Alternative Names

FTE1, MFTL, RPS3A, Small ribosomal subunit protein eS1, 40S ribosomal protein S3a, v-fos transformation effector protein, Fte-1

4 Images

Supplier Data

Western blot - Anti-RPS3A antibody - C-terminal (AB194670)

All lanes:

Western blot - Anti-RPS3A antibody - C-terminal (ab194670) at 1/500 dilution

All lanes:

HeLa cell lysate at 50 µg

Predicted band size: 30 kDa

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Supplier Data

Western blot - Anti-RPS3A antibody - C-terminal (AB194670)

All lanes:

Western blot - Anti-RPS3A antibody - C-terminal (ab194670) at 1/500 dilution

All lanes:

PC-12 cell lysate at 50 µg

Predicted band size: 16 kDa,30 kDa

Observed band size: 13 kDa

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Supplier Data

Western blot - Anti-RPS3A antibody - C-terminal (AB194670)

All lanes:

Western blot - Anti-RPS3A antibody - C-terminal (ab194670) at 1/500 dilution

All lanes:

NIH/3T3 cell lysate at 50 µg

Predicted band size: 30 kDa,33 kDa

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Supplier Data

Western blot - Anti-RPS3A antibody - C-terminal (AB194670)

All lanes:

Western blot - Anti-RPS3A antibody - C-terminal (ab194670) at 1/500 dilution

All lanes:

Raw264.7 cell lysate at 50 µg

Predicted band size: 30 kDa

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Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Rat

Applications

applications

Immunogen

Recombinant Fragment Protein within Human RPS3A aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P61247

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "" }, "Cat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Constituents: 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPS3A also known as Ribosomal Protein S3A is a component of the 40S subunit of the ribosome and has a molecular mass of approximately 29 kDa. The protein interacts directly with mRNA and transfer RNA during protein synthesis. RPS3A is expressed in various tissues including the liver spleen and muscle indicating its widespread presence where protein synthesis is highly active.

Biological function summary

Ribosomal Protein S3A plays a role in the assembly and stability of the small ribosomal subunit. It forms part of the ribosomal complex important for decoding mRNA into polypeptides. RPS3A is also involved in regulating cell growth and proliferation cell cycle progression and apoptosis linking it to cell survival and maintenance. As a ribosomal protein it associates with other ribosomal proteins and rRNA within the ribosome.

Pathways

Ribosomal Protein S3A engages in processes like the mRNA translation pathway and the ribosome biogenesis pathway. It interacts with proteins such as eukaryotic translation initiation factors ensuring the correct initiation and regulation of translation. RPS3A also associates with proteins involved in the mTOR signaling pathway which influences cell growth and metabolism by regulating protein synthesis.

Ribosomal Protein S3A has been implicated in cancer especially hepatocellular carcinoma suggesting a potential role in tumorigenesis. Abnormal expression levels of RPS3A may lead to dysregulated cell proliferation. Additionally RPS3A is associated with Diamond-Blackfan Anemia where changes in its expression could contribute to impaired ribosome function. In these contexts it may interact with proteins like RPL11 and p53 known for their roles in cellular growth regulation and genomic stability.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797). May play a role during erythropoiesis through regulation of transcription factor DDIT3 (By similarity).

See full target information RPS3A

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

eLife 8: PubMed31746735

2019

Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons.

Applications

Unspecified application

Species

Unspecified reactive species

Max Koppers,Roberta Cagnetta,Toshiaki Shigeoka,Lucia Cs Wunderlich,Pedro Vallejo-Ramirez,Julie Qiaojin Lin,Sixian Zhao,Maximilian Ah Jakobs,Asha Dwivedy,Michael S Minett,Anaïs Bellon,Clemens F Kaminski,William A Harris,John G Flanagan,Christine E Holt

Cell 176:56-72.e15 PubMed30612743

2019

Late Endosomes Act as mRNA Translation Platforms and Sustain Mitochondria in Axons.

Applications

Unspecified application

Species

Unspecified reactive species

Jean-Michel Cioni,Julie Qiaojin Lin,Anne V Holtermann,Max Koppers,Maximilian A H Jakobs,Afnan Azizi,Benita Turner-Bridger,Toshiaki Shigeoka,Kristian Franze,William A Harris,Christine E Holt

Frontiers in physiology 8:281 PubMed28533755

2017

Proteomic Analysis of Chicken Skeletal Muscle during Embryonic Development.

Applications

Species

Unspecified reactive species

Hongjia Ouyang,Zhijun Wang,Xiaolan Chen,Jiao Yu,Zhenhui Li,Qinghua Nie

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-RPS3A antibody - C-terminal

Western blot - Anti-RPS3A antibody - C-terminal (AB194670)

Western blot - Anti-RPS3A antibody - C-terminal (AB194670)

Western blot - Anti-RPS3A antibody - C-terminal (AB194670)

Western blot - Anti-RPS3A antibody - C-terminal (AB194670)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise