Rabbit Recombinant Monoclonal RPS3A antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS3A antibody [EPR11982(B)] - BSA and Azide free (AB249646), expandable thumbnail](https://content.abcam.com/products/images/rps3a-antibody-epr11982b-bsa-and-azide-free-ab249646--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img72842.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS3A antibody [EPR11982(B)] - BSA and Azide free (AB249646)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS3A antibody [EPR11982(B)] - BSA and Azide free (AB249646), expandable thumbnail](https://content.abcam.com/products/images/rps3a-antibody-epr11982b-bsa-and-azide-free-ab249646--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img25115.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS3A antibody [EPR11982(B)] - BSA and Azide free (AB249646)")
![Western blot - Anti-RPS3A antibody [EPR11982(B)] - BSA and Azide free (AB249646), expandable thumbnail](https://content.abcam.com/products/images/rps3a-antibody-epr11982b-bsa-and-azide-free-ab249646--western-blot-img116772.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RPS3A antibody [EPR11982(B)] - BSA and Azide free (AB249646)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested |
| Mouse | Predicted | Not recommended | Not recommended | Predicted |
| Rat | Predicted | Not recommended | Not recommended | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:23636399). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797). May play a role during erythropoiesis through regulation of transcription factor DDIT3 (By similarity).
Alternative names
FTE1, MFTL, RPS3A, Small ribosomal subunit protein eS1, 40S ribosomal protein S3a, v-fos transformation effector protein, Fte-1
Recommended products
Rabbit Recombinant Monoclonal RPS3A antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR11982(B)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249646 is the carrier-free version of Anti-RPS3A antibody [EPR11982(B)] ab171742.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RPS3A also known as Ribosomal Protein S3A is a component of the 40S subunit of the ribosome and has a molecular mass of approximately 29 kDa. The protein interacts directly with mRNA and transfer RNA during protein synthesis. RPS3A is expressed in various tissues including the liver spleen and muscle indicating its widespread presence where protein synthesis is highly active.
- Biological function summary
Ribosomal Protein S3A plays a role in the assembly and stability of the small ribosomal subunit. It forms part of the ribosomal complex important for decoding mRNA into polypeptides. RPS3A is also involved in regulating cell growth and proliferation cell cycle progression and apoptosis linking it to cell survival and maintenance. As a ribosomal protein it associates with other ribosomal proteins and rRNA within the ribosome.
- Pathways
Ribosomal Protein S3A engages in processes like the mRNA translation pathway and the ribosome biogenesis pathway. It interacts with proteins such as eukaryotic translation initiation factors ensuring the correct initiation and regulation of translation. RPS3A also associates with proteins involved in the mTOR signaling pathway which influences cell growth and metabolism by regulating protein synthesis.
- Associated diseases and disorders
Ribosomal Protein S3A has been implicated in cancer especially hepatocellular carcinoma suggesting a potential role in tumorigenesis. Abnormal expression levels of RPS3A may lead to dysregulated cell proliferation. Additionally RPS3A is associated with Diamond-Blackfan Anemia where changes in its expression could contribute to impaired ribosome function. In these contexts it may interact with proteins like RPL11 and p53 known for their roles in cellular growth regulation and genomic stability.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS3A antibody [EPR11982(B)] - BSA and Azide free (ab249646), expandable thumbnail](https://content.abcam.com/products/images/rps3a-antibody-epr11982b-bsa-and-azide-free-ab249646--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img72842.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS3A antibody [EPR11982(B)] - BSA and Azide free (ab249646)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS3A antibody [EPR11982(B)] - BSA and Azide free (ab249646)
This data was developed using Anti-RPS3A antibody [EPR11982(B)] ab171742, the same antibody clone in a different buffer formulation.Immunohistochemistry of paraffin-embedded Human pancreas tissue labeling RPS3A using Anti-RPS3A antibody [EPR11982(B)] ab171742 at 1/250 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS3A antibody [EPR11982(B)] - BSA and Azide free (ab249646), expandable thumbnail](https://content.abcam.com/products/images/rps3a-antibody-epr11982b-bsa-and-azide-free-ab249646--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img25115.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS3A antibody [EPR11982(B)] - BSA and Azide free (ab249646)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS3A antibody [EPR11982(B)] - BSA and Azide free (ab249646)
This data was developed using Anti-RPS3A antibody [EPR11982(B)] ab171742, the same antibody clone in a different buffer formulation.Immunohistochemistry of paraffin-embedded Human brain tissue labeling RPS3A using Anti-RPS3A antibody [EPR11982(B)] ab171742 at 1/250 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Western blot - Anti-RPS3A antibody [EPR11982(B)] - BSA and Azide free (ab249646), expandable thumbnail](https://content.abcam.com/products/images/rps3a-antibody-epr11982b-bsa-and-azide-free-ab249646--western-blot-img116772.jpg "Western blot - Anti-RPS3A antibody [EPR11982(B)] - BSA and Azide free (ab249646)")
Western blot - Anti-RPS3A antibody [EPR11982(B)] - BSA and Azide free (ab249646)
This data was developed using Anti-RPS3A antibody [EPR11982(B)] ab171742, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-RPS3A antibody [EPR11982(B)] (Anti-RPS3A antibody [EPR11982(B)] ab171742) at 1/10000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: 293T cell lysate at 10 µg
Lane 3: HepG2 cell lysate at 10 µg
Lane 4: A431 cell lysate at 10 µg
Predicted band size: 30 kDa
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