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AB211427

Anti-RPS4X/SCAR antibody

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(3 Publications)

Rabbit Polyclonal RPS4X/SCAR antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human RPS4X aa 50-150.

View Alternative Names

CCG2, RPS4, SCAR, RPS4X, 40S ribosomal protein S4, SCR10, Single copy abundant mRNA protein

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS4X/SCAR antibody (AB211427)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS4X/SCAR antibody (AB211427)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue labeling RPS4X/SCAR with ab211427 at 10μg/ml.

Immunocytochemistry/ Immunofluorescence - Anti-RPS4X/SCAR antibody (AB211427)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPS4X/SCAR antibody (AB211427)

Immunofluorescent analysis of HUVEC cells labeling RPS4X/SCAR with ab211427 at 1/100 dilution. The image on the right is treated with the synthesized peptide.

Western blot - Anti-RPS4X/SCAR antibody (AB211427)
  • WB

Supplier Data

Western blot - Anti-RPS4X/SCAR antibody (AB211427)

All lanes:

Western blot - Anti-RPS4X/SCAR antibody (ab211427) at 1/500 dilution

Lane 1:

COLO cell lysate

Lane 2:

HeLa cell lysate

Lane 3:

HeLa cell lysate with Synthesized peptide

Predicted band size: 30 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human RPS4X aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

P62701

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Macaque monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPS4X also known as SCAR is a ribosomal protein component of the small 40S subunit with a mass of approximately 29 kDa. Widely expressed RPS4X plays an important role in protein synthesis by contributing to ribosome assembly and function. Its presence is detected in various tissues which indicates its significant involvement in cellular protein production necessary for maintenance and survival.
Biological function summary

Proteins that belong to the ribosomal protein family like RPS4X contribute to the formation of the ribosome structure. RPS4X participates in ribosomal assembly as part of the ribonucleoprotein complex. This role is critical for the synthesis of proteins shaping the cellular proteome and maintaining cellular functions. The ability of RPS4X to incorporate into the ribosome ensures that genetic information from mRNA is translated efficiently into the corresponding polypeptide chains.

Pathways

Protein synthesis requires efficient coordination within the translation process where RPS4X functions. RPS4X is involved in the translation machinery alongside other ribosomal proteins and components like RPS6 and eIFs (eukaryotic initiation factors). These interactions are fundamental within the mRNA translation pathways and link RPS4X to processes such as the cell cycle and growth. Ribosomal proteins like RPS4X ensure proper translational control necessary for growth and development.

Proteins such as RPS4X have implications in ribosome-related dysfunctions. Its altered expression or mutation may associate with conditions like Diamond-Blackfan anemia a disorder characterized by defective erythrocyte production. Additionally anomalies in RPS4X are also linked to Turner syndrome where reduced ribosomal activity could play a contributing role. In both disorders there is a potential connection to ribosomal protein RPS19 which is also integral to ribosome function and pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).
See full target information RPS4X
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 50:2872-2888 PubMed35150276

2022

Genome-wide RNAi screen identifies novel players in human 60S subunit biogenesis including key enzymes of polyamine metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Kerstin Dörner,Lukas Badertscher,Bianka Horváth,Réka Hollandi,Csaba Molnár,Tobias Fuhrer,Roger Meier,Marie Sárazová,Jasmin van den Heuvel,Nicola Zamboni,Peter Horvath,Ulrike Kutay

Journal of Cancer 12:1915-1925 PubMed33753989

2021

Androgen receptor (AR) decreases HCC cells migration and invasion via miR-325/ACP5 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiwu Ouyang,Lemeng Feng,Guodong Liu,Lei Yao,Zhiming Wang,Shiqing Liu,Yao Xiao,Gewen Zhang

Oncology letters 15:3252-3258 PubMed29435066

2018

Potential anticancer effect of prostratin through SIK3 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Dalal Alotaibi,Suneetha Amara,Terrance L Johnson,Venkataswarup Tiriveedhi
View all publications
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