Rabbit Polyclonal RPS4X/SCAR antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human RPS4X aa 50-150.
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Publications
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- Nucleic acids research 50:2872-28882022Genome-wide RNAi screen identifies novel players in human 60S subunit biogenesis including key enzymes of polyamine metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKerstin Dörner,Lukas Badertscher,Bianka Horváth,Réka Hollandi,Csaba Molnár,Tobias Fuhrer,Roger Meier,Marie Sárazová,Jasmin van den Heuvel,Nicola Zamboni,Peter Horvath,Ulrike KutayPubMed 35150276
- Journal of Cancer 12:1915-19252021Androgen receptor (AR) decreases HCC cells migration and invasion via miR-325/ACP5 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiwu Ouyang,Lemeng Feng,Guodong Liu,Lei Yao,Zhiming Wang,Shiqing Liu,Yao Xiao,Gewen ZhangPubMed 33753989
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human RPS4X aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link P62701
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Cat | Predicted | Predicted | Predicted |
| Macaque monkey | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cat, Macaque monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cat, Macaque monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cat, Macaque monkey | Dilution info - | Notes - |
Target data
Function
Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:23636399). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797).
Alternative names
CCG2, RPS4, SCAR, RPS4X, 40S ribosomal protein S4, SCR10, Single copy abundant mRNA protein
Recommended products
Rabbit Polyclonal RPS4X/SCAR antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human RPS4X aa 50-150.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human RPS4X aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link P62701
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RPS4X also known as SCAR is a ribosomal protein component of the small 40S subunit with a mass of approximately 29 kDa. Widely expressed RPS4X plays an important role in protein synthesis by contributing to ribosome assembly and function. Its presence is detected in various tissues which indicates its significant involvement in cellular protein production necessary for maintenance and survival.
- Biological function summary
Proteins that belong to the ribosomal protein family like RPS4X contribute to the formation of the ribosome structure. RPS4X participates in ribosomal assembly as part of the ribonucleoprotein complex. This role is critical for the synthesis of proteins shaping the cellular proteome and maintaining cellular functions. The ability of RPS4X to incorporate into the ribosome ensures that genetic information from mRNA is translated efficiently into the corresponding polypeptide chains.
- Pathways
Protein synthesis requires efficient coordination within the translation process where RPS4X functions. RPS4X is involved in the translation machinery alongside other ribosomal proteins and components like RPS6 and eIFs (eukaryotic initiation factors). These interactions are fundamental within the mRNA translation pathways and link RPS4X to processes such as the cell cycle and growth. Ribosomal proteins like RPS4X ensure proper translational control necessary for growth and development.
- Associated diseases and disorders
Proteins such as RPS4X have implications in ribosome-related dysfunctions. Its altered expression or mutation may associate with conditions like Diamond-Blackfan anemia a disorder characterized by defective erythrocyte production. Additionally anomalies in RPS4X are also linked to Turner syndrome where reduced ribosomal activity could play a contributing role. In both disorders there is a potential connection to ribosomal protein RPS19 which is also integral to ribosome function and pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-RPS4X/SCAR antibody (ab211427)
All lanes: Western blot - Anti-RPS4X/SCAR antibody (ab211427) at 1/500 dilution
Lane 1: COLO cell lysate
Lane 2: HeLa cell lysate
Lane 3: HeLa cell lysate with Synthesized peptide
Predicted band size: 30 kDa
Immunocytochemistry/ Immunofluorescence - Anti-RPS4X/SCAR antibody (ab211427)
Immunofluorescent analysis of HUVEC cells labeling RPS4X/SCAR with ab211427 at 1/100 dilution. The image on the right is treated with the synthesized peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS4X/SCAR antibody (ab211427)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue labeling RPS4X/SCAR with ab211427 at 10μg/ml.
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