Rabbit Polyclonal RPS5 antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human 40S ribosomal protein S5 aa 1-50.
View Alternative Names
Small ribosomal subunit protein uS7, 40S ribosomal protein S5, RPS5
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS5 antibody (AB168823)
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human prostate carcinoma labeling RPS5 with ab168823 at a 1/1000 dilution.
- IP
Supplier Data
Immunoprecipitation - Anti-RPS5 antibody (AB168823)
Samples : Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies : ab168823 used for IP at 6 μg per reaction (Lane 2). RPS5 was also immunoprecipitated by a previous lot of this antibody (Lane 1). For blotting immunoprecipitated RPS5 ab168823 was used at 0.04 μg/ml. Detection : Chemiluminescence with an exposure time of 1 second.
All lanes:
Immunoprecipitation - Anti-RPS5 antibody (ab168823) at 6 mg/mL
All lanes:
HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate (1 mg per IP reaction; 20% of IP loaded)
Predicted band size: 23 kDa
false
- WB
Unknown
Western blot - Anti-RPS5 antibody (AB168823)
All lanes:
Western blot - Anti-RPS5 antibody (ab168823) at 0.1 µg/mL
Lane 1:
293T whole cell lysate at 50 µg
Lane 2:
HeLa whole cell lysate at 50 µg
Lane 3:
Jurkat whole cell lysate at 50 µg
Lane 4:
TCMK-1 whole cell lysate at 50 µg
Lane 5:
NIH3T3 whole cell lysate at 50 µg
Predicted band size: 23 kDa
true
Exposure time: 30s
Reactivity data
Properties and storage information
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Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The protein forms part of the small ribosomal subunit and contributes to the translation of mRNA into proteins. It cooperates with other ribosomal proteins and RNA to ensure the accurate decoding of mRNA sequences during protein synthesis. RPS5 interacts with multiple components of the ribosome to maintain the efficiency and fidelity of translation. It is important for the maturation and assembly of the ribosomal machinery which is essential in cell growth and division.
Pathways
The translation process heavily depends on the activity of RPS5. It participates in the ribosome biogenesis pathway impacting translational control and protein homeostasis. It interacts with RPL5 and several other ribosomal proteins which are important for the proper functioning of the translation process. RPS5 also plays a role in the mTOR signaling pathway which influences cellular growth and metabolism by responding to nutrient and energy status.
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Target data
Publications (1)
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PLoS biology 16:e2005903 PubMed30208026
2018
Applications
Unspecified application
Species
Unspecified reactive species
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