JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB210745

Anti-RPS5 antibody

Be the first to review this product! Submit a review

|

(4 Publications)

Rabbit Polyclonal RPS5 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human 40S ribosomal protein S5 aa 1 to C-terminus.

View Alternative Names

Small ribosomal subunit protein uS7, 40S ribosomal protein S5, RPS5

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-RPS5 antibody (AB210745)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPS5 antibody (AB210745)

Immunofluorescence analysis of U20S cells labeling RPS5 with ab210745 at a 1/50 dilution (green). Nuclei were counterstained with DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS5 antibody (AB210745)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS5 antibody (AB210745)

Immunohistochemical analysis of paraffin-embedded human kidney tissue sections labeling RPS5 with ab210745 at a 1/100 dilution.

Western blot - Anti-RPS5 antibody (AB210745)
  • WB

Supplier Data

Western blot - Anti-RPS5 antibody (AB210745)

All lanes:

Western blot - Anti-RPS5 antibody (ab210745) at 1/500 dilution

Lane 1:

Extracts from MCF7 cell line

Lane 2:

Extracts from HL-60 cell line

Lane 3:

Extracts from HeLa cell line

Lane 4:

Extracts from HepG2 cell line

Lane 5:

Extracts from Jurkat cell line

Lane 6:

Extracts from mouse spleen cell line

Lane 7:

Extracts from mouse lung cell line

Predicted band size: 23 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human 40S ribosomal protein S5 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P46782

style
left-column

Alternative Products

Primary Antibodies

AB168823

Anti-RPS5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS5 antibody (AB168823)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB223089

Anti-RPL23A antibody

Immunocytochemistry/ Immunofluorescence - Anti-RPL23A antibody (AB223089)

  • 0
  • 0
Primary Antibodies

AB241412

Anti-RPL3 antibody

Immunoprecipitation - Anti-RPL3 antibody (AB241412)

  • 0
  • 0
Primary Antibodies

AB174651

Anti-EDF1 antibody

Immunohistochemistry (Frozen sections) - Anti-EDF1 antibody (AB174651)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPS5 also known as 40S ribosomal protein S5 plays an essential role in the structure and function of ribosomes. It is a component of the 40S subunit of ribosomes weighing approximately 21.8 kDa. RPS5 is expressed ubiquitously in a variety of cellular compartments including the cytoplasm where ribosomes are actively involved in protein synthesis. This protein is part of the S7P family of ribosomal proteins and contributes to the assembly and stability of the ribosomal structure.
Biological function summary

The protein forms part of the small ribosomal subunit and contributes to the translation of mRNA into proteins. It cooperates with other ribosomal proteins and RNA to ensure the accurate decoding of mRNA sequences during protein synthesis. RPS5 interacts with multiple components of the ribosome to maintain the efficiency and fidelity of translation. It is important for the maturation and assembly of the ribosomal machinery which is essential in cell growth and division.

Pathways

The translation process heavily depends on the activity of RPS5. It participates in the ribosome biogenesis pathway impacting translational control and protein homeostasis. It interacts with RPL5 and several other ribosomal proteins which are important for the proper functioning of the translation process. RPS5 also plays a role in the mTOR signaling pathway which influences cellular growth and metabolism by responding to nutrient and energy status.

Mutations or dysregulation of RPS5 relate to cancer and Diamond-Blackfan anemia. In certain cancers changes in ribosomal proteins like RPS5 disrupt normal protein synthesis contributing to unchecked cell proliferation. In Diamond-Blackfan anemia insufficient production of ribosomal proteins including RPS5 leads to impaired red blood cell production. RPS5 in conjunction with other ribosomal proteins becomes a focal point to study these diseases for potential therapeutic targets.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Component of the small ribosomal subunit (PubMed : 23636399). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).
See full target information 40S ribosomal protein S5
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 134: PubMed38875287

2024

Liver cancer initiation requires translational activation by an oncofetal regulon involving LIN28 proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Hsiung Hsieh,Yonglong Wei,Lin Li,Liem H Nguyen,Yu-Hsuan Lin,Jung M Yong,Xuxu Sun,Xun Wang,Xin Luo,Sarah K Knutson,Christina Bracken,George Q Daley,John T Powers,Hao Zhu

Communications biology 5:564 PubMed35681048

2022

Stabilization of CDK6 by ribosomal protein uS7, a target protein of the natural product fucoxanthinol.

Applications

Unspecified application

Species

Unspecified reactive species

Yosuke Iizumi,Yoshihiro Sowa,Wakana Goi,Yuichi Aono,Motoki Watanabe,Yoichi Kurumida,Tomoshi Kameda,Kenichi Akaji,Masatoshi Kitagawa,Toshiyuki Sakai

Virology 573:50-58 PubMed35714458

2022

Integrated proteomics and transcriptomics analyses identify novel cell surface markers of HIV latency.

Applications

Unspecified application

Species

Unspecified reactive species

Nadejda Beliakova-Bethell,Antigoni Manousopoulou,Savitha Deshmukh,Amey Mukim,Douglas D Richman,Spiros D Garbis,Celsa A Spina

EMBO reports 22:e52170 PubMed34605609

2021

Muscle-specific Cand2 is translationally upregulated by mTORC1 and promotes adverse cardiac remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Agnieszka A Górska,Clara Sandmann,Eva Riechert,Christoph Hofmann,Ellen Malovrh,Eshita Varma,Vivien Kmietczyk,Julie Ölschläger,Lonny Jürgensen,Verena Kamuf-Schenk,Claudia Stroh,Jennifer Furkel,Mathias H Konstandin,Carsten Sticht,Etienne Boileau,Christoph Dieterich,Norbert Frey,Hugo A Katus,Shirin Doroudgar,Mirko Völkers
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com