AB225676

Anti-RPS6 antibody [EPR22168] - Ribosome Marker

Name: Anti-RPS6 antibody - Ribosome Marker [EPR22168] (ab225676) | Abcam
Brand: Abcam Limited
SKU: AB225676
Price: 245 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
Lab Essentials
20ul selling size
What is this?

(1 Review)

(7 Publications)

Rabbit Recombinant Monoclonal RPS6 antibody. Ribosome marker. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 7 publications.

View Alternative Names

OK/SW-cl.2, RPS6, Small ribosomal subunit protein eS6, 40S ribosomal protein S6, Phosphoprotein NP33

9 Images

Flow Cytometry (Intracellular) - Anti-RPS6 antibody [EPR22168] - Ribosome Marker (AB225676)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RPS6 antibody [EPR22168] - Ribosome Marker (AB225676)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling RPS6 with ab225676 at 1/600 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 antibody [EPR22168] - Ribosome Marker (AB225676)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 antibody [EPR22168] - Ribosome Marker (AB225676)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling RPS6 with ab225676 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in human breast cancer (PMID : 22698676). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-RPS6 antibody [EPR22168] - Ribosome Marker (AB225676)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPS6 antibody [EPR22168] - Ribosome Marker (AB225676)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling RPS6 with ab225676 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in the HeLa cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 antibody [EPR22168] - Ribosome Marker (AB225676)

Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling RPS6 with ab225676 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining predominantly in neurons of the human cerebral cortex (PMID : 26432888). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPS6 antibody [EPR22168] - Ribosome Marker (AB225676)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryo fibroblast cell line) cells labeling RPS6 with ab225676 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in the NIH/3T3 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 antibody [EPR22168] - Ribosome Marker (AB225676)

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling RPS6 with ab225676 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining predominantly in neurons of the rat cerebellum (PMID : 26432888). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 antibody [EPR22168] - Ribosome Marker (AB225676)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling RPS6 with ab225676 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining predominantly in neurons of the mouse cerebrum (PMID : 26432888). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-RPS6 antibody [EPR22168] - Ribosome Marker (AB225676)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RPS6 antibody [EPR22168] - Ribosome Marker (ab225676) at 1/1000 dilution

Lane 1:

Mouse heart lysate at 10 µg

Lane 2:

Mouse kidney lysate at 10 µg

Lane 3:

Mouse spleen lysate at 10 µg

Lane 4:

Rat brain lysate at 10 µg

Lane 5:

Human brain lysate at 10 µg

Lane 6:

Human fetal heart lysate at 10 µg

Lane 7:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-RPS6 antibody [EPR22168] - Ribosome Marker (AB225676)

Exposure time : Lanes 1-6 : 26 seconds; Lane 7 : 10 seconds.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RPS6 antibody [EPR22168] - Ribosome Marker (ab225676) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 3:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Lane 4:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 5:

HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 20 µg

Lane 6:

PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg

Lane 7:

NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Carrier free

Anti-RPS6 antibody [EPR22168] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-RPS6 antibody [EPR22168] - Ribosome Marker
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-RPS6 antibody [EPR22168] - Ribosome Marker
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-RPS6 antibody [EPR22168] - Ribosome Marker
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-RPS6 antibody [EPR22168] - Ribosome Marker
578 PE

PE Anti-RPS6 antibody [EPR22168] - Ribosome Marker
660 APC

APC Anti-RPS6 antibody [EPR22168] - Ribosome Marker
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-RPS6 antibody [EPR22168] - Ribosome Marker

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22168

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/600", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPS6 also known as Ribosomal Protein S6 is a component of the 40S ribosomal subunit with a molecular weight of approximately 28 kDa. This protein mainly localizes in the cytoplasm where ribosomes are fixed during protein synthesis. The ribosomal machinery uses RPS6 to translate mRNA into polypeptides operating as an essential part of protein biosynthesis. It expresses in various tissues with higher levels in highly proliferative cells which need extensive protein production for growth.

Biological function summary

RPS6 is integral to the formation of the ribosomal complex. This complex is essential for the translation initiation process. RPS6 controls the translation capacity of ribosomes and functions in growth and development by regulating protein synthesis rates. Alterations in RPS6 activity can impact cellular growth and size due to its role in ribosome biogenesis.

Pathways

RPS6 plays a significant role in the mTOR and PI3K/AKT signaling pathways. These pathways link nutrient availability to protein synthesis influencing cellular growth and metabolism. RPS6 interacts with proteins like S6K1 which is a downstream target in the mTOR pathway to modulate translation and cell growth processes.

RPS6 has connections to cancer and metabolic syndromes. Abnormal RPS6 activation can lead to dysregulated cell growth contributing to oncogenesis. In the context of cancer mTOR signaling where RPS6 functions is often upregulated leading to uncontrolled proliferation. Moreover in metabolic disorders the disrupted pathway activity involves other proteins like AKT fostering insulin resistance and metabolic imbalance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the 40S small ribosomal subunit (PubMed : 23636399, PubMed : 8706699). Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA (PubMed : 17220279). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).

See full target information RPS6

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

The Journal of physiology 603:5529-5545 PubMed40946331

2025

Clenbuterol induces lean mass and muscle protein accretion, but attenuates cardiorespiratory fitness and desensitizes muscle β-adrenergic signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Morten Hostrup,Lukas Moesgaard,Mads Fischer,Kate Aiko Wickham,Mads Pleshardt,Andreas Breenfeldt Andersen,Jacob Bejder,Martin Thomassen,Jens J Nielsen,Yvette Dehnes,Jens Bangsbo,Nikolai B Nordsborg,Søren Jessen

International urogynecology journal 36:1523-1531 PubMed40478270

2025

Expressions of IGF-1, pAKT, and pS6 in Uterosacral Ligaments of Patients with Pelvic Organ Prolapse.

Applications

Unspecified application

Species

Unspecified reactive species

Hongling Xu,Gensheng Wang,Qing Li,Xuemei Wang,Dongdi Xu

Nucleic acids research 53: PubMed39898547

2025

The short conserved region-2 of LARP4 interacts with ribosome-associated RACK1 and promotes translation.

Applications

Unspecified application

Species

Unspecified reactive species

Amitabh Ranjan,Sandy Mattijssen,Nithin Charlly,Isabel Cruz Gallardo,Leah F Pitman,Jennifer C Coleman,Maria R Conte,Richard J Maraia

Journal of cellular and molecular medicine 26:5713-5727 PubMed36308410

2022

Phosphorylation-mediated PI3K-Art signalling pathway as a therapeutic mechanism in the hydrogen-induced alleviation of brain injury in septic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Bai,Li Li,Beibei Dong,Wanjie Ma,Hongguang Chen,Yonghao Yu

Current cancer drug targets 23:307-318 PubMed36200259

2022

VX-765 has a Protective Effect on Mice with Ovarian Injury Caused by Chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Pingyin Lee,Canquan Zhou,Xiaokun Hu

Oncology reports 45: PubMed33907854

2021

Knockdown of SASS6 reduces growth of MDA‑MB‑231 triple‑negative breast cancer cells through arrest of the cell cycle at the G2/M phase.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Du,Jiexian Jing,Yan Wang,Xiaoqin Xu,Ting Sun,Yanchun Shi,Weigang Wang,Baoguo Tian,Cunzhi Han,Xianwen Zhao,Huibo Chang

Journal of Cancer 10:1855-1861 PubMed31205542

2019

BTF3 Silencing Inhibits the Proliferation of Osteosarcoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Liu,Lin Jiang,Wanchun Wang,Tao Jiang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPS6 antibody [EPR22168] - Ribosome Marker

Flow Cytometry (Intracellular) - Anti-RPS6 antibody [EPR22168] - Ribosome Marker (AB225676)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 antibody [EPR22168] - Ribosome Marker (AB225676)

Immunocytochemistry/ Immunofluorescence - Anti-RPS6 antibody [EPR22168] - Ribosome Marker (AB225676)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 antibody [EPR22168] - Ribosome Marker (AB225676)

Immunocytochemistry/ Immunofluorescence - Anti-RPS6 antibody [EPR22168] - Ribosome Marker (AB225676)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 antibody [EPR22168] - Ribosome Marker (AB225676)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 antibody [EPR22168] - Ribosome Marker (AB225676)

Western blot - Anti-RPS6 antibody [EPR22168] - Ribosome Marker (AB225676)

Western blot - Anti-RPS6 antibody [EPR22168] - Ribosome Marker (AB225676)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise