Rabbit Polyclonal RPS6 antibody. Ribosome marker. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 47 publications.
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- PLoS pathogens 19:e10117482023Divergence of TORC1-mediated stress response leads to novel acquired stress resistance in a pathogenic yeast.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinye Liang,Hanxi Tang,Lindsey F Snyder,Christopher E Youngstrom,Bin Z HePubMed 37871123
- EMBO molecular medicine 15:e164342023Interferon regulates neural stem cell function at all ages by orchestrating mTOR and cell cycle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDamian Carvajal Ibañez,Maxim Skabkin,Jooa Hooli,Santiago Cerrizuela,Manuel Göpferich,Adrien Jolly,Katrin Volk,Marc Zumwinkel,Matilde Bertolini,Gianluca Figlia,Thomas Höfer,Guenter Kramer,Simon Anders,Aurelio A Teleman,Anna Marciniak-Czochra,Ana Martin-VillalbaPubMed 36636818
- Journal of cell science 135:2022Conserved Pib2 regions have distinct roles in TORC1 regulation at the vacuole.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKayla K Troutman,Natalia V Varlakhanova,Bryan A Tornabene,Rajesh Ramachandran,Marijn G J FordPubMed 36000409
- Journal of advanced research 39:1-132022Salicylic acid fights against Fusarium wilt by inhibiting target of rapamycin signaling pathway in Fusarium oxysporum.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLinxuan Li,Tingting Zhu,Yun Song,Li Feng,Philip James Kear,Rooallah Saberi Riseh,Mahmoud Sitohy,Raju Datla,Maozhi RenPubMed 35777900
- mBio 13:e01420222022S. cerevisiae Cells Can Grow without the Pds5 Cohesin Subunit.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaran Choudhary,Ziv Itzkovich,Elisa Alonso-Perez,Hend Bishara,Barbara Dunn,Gavin Sherlock,Martin KupiecPubMed 35708277
- Development (Cambridge, England) 149:2022Msl3 promotes germline stem cell differentiation in female Drosophila.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlicia McCarthy,Kahini Sarkar,Elliot T Martin,Maitreyi Upadhyay,Seoyeon Jang,Nathan D Williams,Paolo E Forni,Michael Buszczak,Prashanth RanganPubMed 34878097
- Non-coding RNA 7:2021tRNA Synthetases Are Recruited to Yeast Ribosomes by rRNA Expansion Segment 7L but Do Not Require Association for Functionality.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNina Krauer,Robert Rauscher,Norbert PolacekPubMed 34842814
- eLife 10:2021One-shot analysis of translated mammalian lncRNAs with AHARIBO.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuca Minati,Claudia Firrito,Alessia Del Piano,Alberto Peretti,Simone Sidoli,Daniele Peroni,Romina Belli,Francesco Gandolfi,Alessandro Romanel,Paola Bernabo,Jacopo Zasso,Alessandro Quattrone,Graziano Guella,Fabio Lauria,Gabriella Viero,Massimiliano ClamerPubMed 33594971
- Pesticide biochemistry and physiology 173:1047742021The VKORC1 ER-luminal loop mutation (Leu76Pro) leads to a significant resistance to warfarin in black rats (Rattus rattus).Applications:Unspecified applicationReactive species:Unspecified reactive speciesKazuki Takeda,Yoshinori Ikenaka,Denis Fourches,Kazuyuki D Tanaka,Shouta M M Nakayama,Dhoha Triki,Xinhao Li,Manabu Igarashi,Tsutomu Tanikawa,Mayumi IshizukaPubMed 33771253
- Autophagy 17:2939-29612020A VASt-domain protein regulates autophagy, membrane tension, and sterol homeostasis in rice blast fungus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXue-Ming Zhu,Lin Li,Ying-Ying Cai,Xi-Yu Wu,Huan-Bin Shi,Shuang Liang,Ying-Min Qu,Naweed I Naqvi,Maurizio Del Poeta,Bo Dong,Fu-Cheng Lin,Xiao-Hong LiuPubMed 33176558
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Saccharomyces cerevisiae | Predicted | Predicted | Predicted |
| Schizosaccharomyces pombe | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Saccharomyces cerevisiae, Schizosaccharomyces pombe | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Saccharomyces cerevisiae, Schizosaccharomyces pombe | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Saccharomyces cerevisiae, Schizosaccharomyces pombe | Dilution info - | Notes - |
Associated Products
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Target data
Function
Component of the 40S small ribosomal subunit (PubMed:23636399, PubMed:8706699). Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA (PubMed:17220279). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797).
Alternative names
OK/SW-cl.2, RPS6, Small ribosomal subunit protein eS6, 40S ribosomal protein S6, Phosphoprotein NP33
Recommended products
Rabbit Polyclonal RPS6 antibody. Ribosome marker. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 47 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
Replenishment batches of our polyclonal antibody, ab40820 are tested in WB. Previous batches were additionally validated in ICC/IF and IHC-P. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab225676.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RPS6 also known as Ribosomal Protein S6 is a component of the 40S ribosomal subunit with a molecular weight of approximately 28 kDa. This protein mainly localizes in the cytoplasm where ribosomes are fixed during protein synthesis. The ribosomal machinery uses RPS6 to translate mRNA into polypeptides operating as an essential part of protein biosynthesis. It expresses in various tissues with higher levels in highly proliferative cells which need extensive protein production for growth.
- Biological function summary
RPS6 is integral to the formation of the ribosomal complex. This complex is essential for the translation initiation process. RPS6 controls the translation capacity of ribosomes and functions in growth and development by regulating protein synthesis rates. Alterations in RPS6 activity can impact cellular growth and size due to its role in ribosome biogenesis.
- Pathways
RPS6 plays a significant role in the mTOR and PI3K/AKT signaling pathways. These pathways link nutrient availability to protein synthesis influencing cellular growth and metabolism. RPS6 interacts with proteins like S6K1 which is a downstream target in the mTOR pathway to modulate translation and cell growth processes.
- Associated diseases and disorders
RPS6 has connections to cancer and metabolic syndromes. Abnormal RPS6 activation can lead to dysregulated cell growth contributing to oncogenesis. In the context of cancer mTOR signaling where RPS6 functions is often upregulated leading to uncontrolled proliferation. Moreover in metabolic disorders the disrupted pathway activity involves other proteins like AKT fostering insulin resistance and metabolic imbalance.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
This image is courtesy of a customer review submitted by Antibody Solutions Ltd.Western blot - Anti-RPS6 antibody - Ribosome Marker (ab40820)
Detection method: Supersignal West Dura
All lanes: Western blot - Anti-RPS6 antibody - Ribosome Marker (ab40820) at 2 µg/mL
Lane 1: Whole cell lysate from mouse NIH-3T3 cell line at 30 µg
Lane 2: Whole cell lysate from mouse MEF cell line at 30 µg
Lane 3: Whole cell lysate from rat PC12 cell line at 30 µg
SecondaryAll lanes: HRP-conjugated pig anti rabbit Ig at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 29 kDa
Exposure time: 5min
Western blot - Anti-RPS6 antibody - Ribosome Marker (ab40820)
All lanes: Western blot - Anti-RPS6 antibody - Ribosome Marker (ab40820) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 3: A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 4: HEK293 Human embryonic kidney cell line Whole Cell Lysate at 10 µg
Lane 5: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 6: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Lane 7: SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 31 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 antibody - Ribosome Marker (ab40820)
IHC image of RPS6 staining in a section of formalin-fixed paraffin-embedded normal human skin melanoma* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab40820, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Immunocytochemistry/ Immunofluorescence - Anti-RPS6 antibody - Ribosome Marker (ab40820)
ab40820 stained in A431 cells. Cells were fixed with 100% methanol (5 min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% Triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab40820 at 5μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse monoclonal to alpha Tubulin - Loading Control) used at a 1/1000 dilution overnight at +4°C. The secondary antibodies were Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 μM for 1hour at room temperature.
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