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AB70227

Anti-RPS6 antibody - Ribosome Marker

5

(2 Reviews)

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(14 Publications)

Rabbit Polyclonal RPS6 antibody. Ribosome marker. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human RPS6.

View Alternative Names

OK/SW-cl.2, RPS6, Small ribosomal subunit protein eS6, 40S ribosomal protein S6, Phosphoprotein NP33

4 Images
Immunoprecipitation - Anti-RPS6 antibody - Ribosome Marker (AB70227)
  • IP

Unknown

Immunoprecipitation - Anti-RPS6 antibody - Ribosome Marker (AB70227)

Detection of Human RPS6 by Immunoprecipitation. Samples : A) Whole cell lysate from HeLa cells (1 mg/IP; 1/4 of IP loaded) using affinity purified ab70227 at 3 μg/mg lysate for IP and at 0.04 μg/ml for subequent WB detection. Second lane represents IgG IP control.

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Immunoprecipitation - Anti-RPS6 antibody - Ribosome Marker (ab70227)

Predicted band size: 29 kDa

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Western blot - Anti-RPS6 antibody - Ribosome Marker (AB70227)
  • WB

Unknown

Western blot - Anti-RPS6 antibody - Ribosome Marker (AB70227)

All lanes:

Western blot - Anti-RPS6 antibody - Ribosome Marker (ab70227) at 0.04 µg/mL

Lane 1:

Whole cell lysate from HeLa cells at 50 µg

Lane 2:

Whole cell lysate from HeLa cells at 15 µg

Lane 3:

Whole cell lysate from HeLa cells at 5 µg

Lane 4:

Whole cell lysate from 293T cells at 50 µg

Lane 5:

Whole cell lysate from NIH3T3 cells at 50 µg

Predicted band size: 29 kDa

Observed band size: 30 kDa

false

Exposure time: 30s

Western blot - Anti-RPS6 antibody - Ribosome Marker (AB70227)
  • WB

AbReview41962****

Western blot - Anti-RPS6 antibody - Ribosome Marker (AB70227)

All lanes:

Western blot - Anti-RPS6 antibody - Ribosome Marker (ab70227) at 1/1000 dilution

All lanes:

Caenorhabditis elegans whole adult tissue lysate at 200 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution

Predicted band size: 29 kDa

Observed band size: 24 kDa,26 kDa,28 kDa,40 kDa

true

Exposure time: 40s

This image is courtesy of an anonymous Abreview

Western blot - Anti-RPS6 antibody - Ribosome Marker (AB70227)
  • WB

CiteAb

Western blot - Anti-RPS6 antibody - Ribosome Marker (AB70227)

RPS6 western blot using anti-RPS6 antibody - Ribosome Marker ab70227. Publication image and figure legend from Tiku, V., Jain, C., et al., 2017, Nat Commun, PubMed 28853436.

ab70227 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab70227 please see the product overview.

Longevity mutants have reduced ribosome biogenesis.(a) FIB-1 : : GFP is strongly down-regulated in eat-2(ad465) animals but up-regulated in ncl-1(e1942) and eat-2;ncl-1 double mutants (20 worms imaged per replicate, 3 independent biological replicates). (b–d) rRNA, RPS6, RPS15 and FIB-1 levels are increased in ncl-1(e1942) and reduced in eat-2(ad465), isp-1(qm150), glp-1(e2141), daf-2 RNAi and TOR RNAi and this effect is partially reversed by the loss of ncl-1. daf-2(e1370) also shows reduced rRNA levels compared to daf-2;daf-16(mu86) (Error bars represent mean±s.e.m.). (e) fib-1 RNAi extends lifespan of N2 (P=0.0004, log-rank test, three independent biological replicates) (f) fib-1 RNAi reduces the nucleolar size of N2 (Error bars represent mean±s.d.). Scale bar represents 20 μm. *p<0.05, **p<0.01, ****p<0.0001, unpaired t-test.

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human RPS6. The exact immunogen used to generate this antibody is proprietary information.

P62753

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Goat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Lizard": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPS6 also known as Ribosomal Protein S6 is a component of the 40S ribosomal subunit with a molecular weight of approximately 28 kDa. This protein mainly localizes in the cytoplasm where ribosomes are fixed during protein synthesis. The ribosomal machinery uses RPS6 to translate mRNA into polypeptides operating as an essential part of protein biosynthesis. It expresses in various tissues with higher levels in highly proliferative cells which need extensive protein production for growth.
Biological function summary

RPS6 is integral to the formation of the ribosomal complex. This complex is essential for the translation initiation process. RPS6 controls the translation capacity of ribosomes and functions in growth and development by regulating protein synthesis rates. Alterations in RPS6 activity can impact cellular growth and size due to its role in ribosome biogenesis.

Pathways

RPS6 plays a significant role in the mTOR and PI3K/AKT signaling pathways. These pathways link nutrient availability to protein synthesis influencing cellular growth and metabolism. RPS6 interacts with proteins like S6K1 which is a downstream target in the mTOR pathway to modulate translation and cell growth processes.

RPS6 has connections to cancer and metabolic syndromes. Abnormal RPS6 activation can lead to dysregulated cell growth contributing to oncogenesis. In the context of cancer mTOR signaling where RPS6 functions is often upregulated leading to uncontrolled proliferation. Moreover in metabolic disorders the disrupted pathway activity involves other proteins like AKT fostering insulin resistance and metabolic imbalance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the 40S small ribosomal subunit (PubMed : 23636399, PubMed : 8706699). Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA (PubMed : 17220279). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).
See full target information RPS6
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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Cell reports methods 3:100433 PubMed37056370

2023

A single-copy knockin translating ribosome immunoprecipitation toolkit for tissue-specific profiling of actively translated mRNAs in . .

Applications

Unspecified application

Species

Unspecified reactive species

Laura E Wester,Anne Lanjuin,Emanuel H W Bruckisch,Maria C Perez-Matos,Peter G Stine,Caroline Heintz,Martin S Denzel,William B Mair

Heliyon 9:e15305 PubMed37101626

2023

EEF1A2 accelerates the protein translation of chemokine in rat myocardial cells induced by ischemia-reperfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Zi-Jie Zhang,Zhi-Xiang Sun,Hai-Jian Liu

International journal of biological sciences 18:4809-4823 PubMed35982897

2022

The Novel Role of hnRNP UL1 in Human Cell Nucleoli.

Applications

Unspecified application

Species

Unspecified reactive species

Marlena Cichocka,Anna Karlik,Patrycja Plewka,Kishor Gawade,Agata Stępień,Patrycja Świergiel,Ankur Gadgil,Katarzyna Dorota Raczyńska

PloS one 16:e0262180 PubMed34972198

2021

The TOR kinase pathway is relevant for nitrogen signaling and antagonism of the mycoparasite Trichoderma atroviride.

Applications

Unspecified application

Species

Unspecified reactive species

Rossana Segreto,Hoda Bazafkan,Julia Millinger,Martina Schenk,Lea Atanasova,Maria Doppler,Christoph Büschl,Mélanie Boeckstaens,Silvia Soto Diaz,Ulrike Schreiner,Fabiano Sillo,Raffaella Balestrini,Rainer Schuhmacher,Susanne Zeilinger

Cancer biology & medicine : PubMed33710804

2021

SNORA23 inhibits HCC tumorigenesis by impairing the 2'-O-ribose methylation level of 28S rRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyong Liu,Yanan Pang,Yin Jia,Qin Qin,Rui Wang,Wei Li,Jie Jing,Haidong Liu,Shanrong Liu

Medicines (Basel, Switzerland) 8: PubMed33535532

2021

The Infantile Leukoencephalopathy-Associated Mutation of C11ORF73/HIKESHI Proteins Generates de novo Interactive Activity with Filamin A, Inhibiting Oligodendroglial Cell Morphological Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Kohei Hattori,Kenji Tago,Shiori Memezawa,Arisa Ochiai,Sui Sawaguchi,Yukino Kato,Takanari Sato,Kazuma Tomizuka,Hiroaki Ooizumi,Katsuya Ohbuchi,Kazushige Mizoguchi,Yuki Miyamoto,Junji Yamauchi

Journal of ovarian research 13:100 PubMed32862831

2020

Knockdown of ribosomal protein S6 suppresses proliferation, migration, and invasion in epithelial ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xueqing Yang,Luxi Xu,Yu-E Yang,Chang Xiong,Jinjin Yu,Yuan Wang,Yaying Lin

Biomedicines 8: PubMed32316234

2020

PP1C and PP2A are p70S6K Phosphatases Whose Inhibition Ameliorates HLD12-Associated Inhibition of Oligodendroglial Cell Morphological Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Naoto Matsumoto,Yuki Miyamoto,Kohei Hattori,Akihiro Ito,Hironori Harada,Hiroaki Oizumi,Katsuya Ohbuchi,Kazushige Mizoguchi,Junji Yamauchi

Haematologica : PubMed31699789

2019

ASLAN003, a potent dihydroorotate dehydrogenase inhibitor for differentiation of acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Jianbiao Zhou,Jessie Yiying Quah,Yvonne Ng,Jing-Yuan Chooi,Sabrina Hui-Min Toh,Baohong Lin,Tuan Zea Tan,Hiroki Hosoi,Motomi Osato,Qihui Seet,A G Lisa Ooi,Bertil Lindmark,Mark McHale,Wee-Joo Chng

PLoS genetics 15:e1008144 PubMed31086376

2019

A long noncoding RNA distributed in both nucleus and cytoplasm operates in the PYCARD-regulated apoptosis by coordinating the epigenetic and translational regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Miao,Linlin Wang,Haomiao Zhan,Jiangshan Dai,Yanbo Chang,Fan Wu,Tao Liu,Zhongyu Liu,Chunfang Gao,Ling Li,Xu Song
View all publications
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