AB227005

Anti-RPS6 (phospho S235) antibody - C-terminal

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(1 Publication)

Rabbit Polyclonal RPS6 phospho S235 antibody. C-terminal. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RPS6 pS235.

View Alternative Names

OK/SW-cl.2, RPS6, Small ribosomal subunit protein eS6, 40S ribosomal protein S6, Phosphoprotein NP33

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235) antibody - C-terminal (AB227005)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235) antibody - C-terminal (AB227005)

Paraffin-embedded mouse brain tissue stained for RPS6 (phospho S235) using ab227005 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-RPS6 (phospho S235) antibody - C-terminal (AB227005)

12% SDS-PAGE gel.

All lanes:

Western blot - Anti-RPS6 (phospho S235) antibody - C-terminal (ab227005) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen), starved for 16 hours, whole cell extract at 40 µg

Lane 2:

HEK-293T, starved for 16 hours and treated with 100 nM Rapamycin for 30 minutes, whole cell extract at 40 µg

Lane 3:

HEK-293T, starved for 16 hours and treated with 100 nM Insulin for 30 minutes, whole cell extract at 40 µg

Lane 4:

HEK-293T, starved for 16 hours and treated with 100 nM Rapamycin for 30 minutes and 100 nM Insulin for 30 minutes, whole cell extract at 40 µg

Predicted band size: 29 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human RPS6 pS235. The exact immunogen used to generate this antibody is proprietary information.

P62753

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPS6 also known as Ribosomal Protein S6 is a component of the 40S ribosomal subunit with a molecular weight of approximately 28 kDa. This protein mainly localizes in the cytoplasm where ribosomes are fixed during protein synthesis. The ribosomal machinery uses RPS6 to translate mRNA into polypeptides operating as an essential part of protein biosynthesis. It expresses in various tissues with higher levels in highly proliferative cells which need extensive protein production for growth.

Biological function summary

RPS6 is integral to the formation of the ribosomal complex. This complex is essential for the translation initiation process. RPS6 controls the translation capacity of ribosomes and functions in growth and development by regulating protein synthesis rates. Alterations in RPS6 activity can impact cellular growth and size due to its role in ribosome biogenesis.

Pathways

RPS6 plays a significant role in the mTOR and PI3K/AKT signaling pathways. These pathways link nutrient availability to protein synthesis influencing cellular growth and metabolism. RPS6 interacts with proteins like S6K1 which is a downstream target in the mTOR pathway to modulate translation and cell growth processes.

RPS6 has connections to cancer and metabolic syndromes. Abnormal RPS6 activation can lead to dysregulated cell growth contributing to oncogenesis. In the context of cancer mTOR signaling where RPS6 functions is often upregulated leading to uncontrolled proliferation. Moreover in metabolic disorders the disrupted pathway activity involves other proteins like AKT fostering insulin resistance and metabolic imbalance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the 40S small ribosomal subunit (PubMed : 23636399, PubMed : 8706699). Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA (PubMed : 17220279). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).

See full target information RPS6 pS235

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112634 PubMed40502711

2025

Cytohesin-4/ARF6 facilitates the progression of acute myeloid leukemia through activating PIK3R5/PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Fen Qiu,Cheng-Ming He,Yan-Mei Zeng,Xiao-Ling Deng,Guo-Lin Liang,Ming-Xing Zhong,Min Zou,Xiu-Juan Xiong,Jing-Dong Zhang,Yan Ye,Qing Niu,Xiao-Li Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPS6 (phospho S235) antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235) antibody - C-terminal (AB227005)

Western blot - Anti-RPS6 (phospho S235) antibody - C-terminal (AB227005)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise