AB213165

Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal RPS6 phospho S235 antibody. Carrier free. Suitable for IP, Dot, WB, IHC-P and reacts with Human, Synthetic peptide samples. Cited in 1 publication.

View Alternative Names

OK/SW-cl.2, RPS6, Small ribosomal subunit protein eS6, 40S ribosomal protein S6, Phosphoprotein NP33

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - BSA and Azide free (AB213165)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - BSA and Azide free (AB213165)

ab80158 at 1/100-1/250 dilution staining RPS6 in human breast adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab80158).

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Lab

Immunoprecipitation - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - BSA and Azide free (AB213165)

This data was developed using ab80158, the same antibody clone in a different buffer formulation.

RPS6 was immunoprecipitated from 0.35 mg 293T (Human embryonic kidney epithelial cell) treated with Calyculin A (100nM 30min) whole cell lysate 10 μg with ab80158 at 1/50 dilution (2μg). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : 293T (Human embryonic kidney epithelial cell) treated with Calyculin A (100nM 30min) whole cell lysate 10 μg

Lane 2 : ab80158 IP in 293T treated with Calyculin A (100nM 30min) whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab80158 in 293T treated with Calyculin A (100nM 30min) whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (<a href='/en-us/products/primary-antibodies/rps6-phospho-s235-antibody-ep13382y-ribosome-marker-ab80158'>ab80158</a>)

Predicted band size: 29 kDa

Observed band size: 32 kDa

false

Unknown

Dot Blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - BSA and Azide free (AB213165)

Dot Blot analysis of -

Lane 1 : Rb (pS235) phospho peptide

Lane 2 : Rb (pS236) phospho peptide

Lane 3 : Rb (pS235 & pS236) phospho peptide

Lane 4 : Rb non-phospho peptide

5% NFDM/TBST was used as the diluting and blocking buffer.

ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time : 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab80158).

Unconjugated

Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1338(2)Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP, IHC-P, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab213165 is the carrier-free version of ab80158.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPS6 also known as Ribosomal Protein S6 is a component of the 40S ribosomal subunit with a molecular weight of approximately 28 kDa. This protein mainly localizes in the cytoplasm where ribosomes are fixed during protein synthesis. The ribosomal machinery uses RPS6 to translate mRNA into polypeptides operating as an essential part of protein biosynthesis. It expresses in various tissues with higher levels in highly proliferative cells which need extensive protein production for growth.

Biological function summary

RPS6 is integral to the formation of the ribosomal complex. This complex is essential for the translation initiation process. RPS6 controls the translation capacity of ribosomes and functions in growth and development by regulating protein synthesis rates. Alterations in RPS6 activity can impact cellular growth and size due to its role in ribosome biogenesis.

Pathways

RPS6 plays a significant role in the mTOR and PI3K/AKT signaling pathways. These pathways link nutrient availability to protein synthesis influencing cellular growth and metabolism. RPS6 interacts with proteins like S6K1 which is a downstream target in the mTOR pathway to modulate translation and cell growth processes.

RPS6 has connections to cancer and metabolic syndromes. Abnormal RPS6 activation can lead to dysregulated cell growth contributing to oncogenesis. In the context of cancer mTOR signaling where RPS6 functions is often upregulated leading to uncontrolled proliferation. Moreover in metabolic disorders the disrupted pathway activity involves other proteins like AKT fostering insulin resistance and metabolic imbalance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the 40S small ribosomal subunit (PubMed : 23636399, PubMed : 8706699). Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA (PubMed : 17220279). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).

See full target information RPS6 phospho S235

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:5728 PubMed40593621

2025

HDL-bound S1P affects the subventricular niche and early neuropathological features of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Byung Jo Choi,Ju Yeon Hong,Min Hee Park,Kang Ho Park,Wan Hui Han,Hee Ji Yoon,Hye Yoon Jung,Kyung Yeol Kim,Sun Ae Lee,Eun Young Lim,Jung Woo Hur,Im-Sook Song,So Yeon Jeon,Min-Koo Choi,Christina Christoffersen,Hee-Jin Kim,Seung Hyun Kim,Edward H Schuchman,Jae-Sung Bae,Hee Kyung Jin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - BSA and Azide free (AB213165)

Immunoprecipitation - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - BSA and Azide free (AB213165)

Dot Blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - BSA and Azide free (AB213165)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Properties and storage information

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Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

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Product protocols

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Publications (1)

Product promise