Rabbit Recombinant Monoclonal RPS6 phospho S235 antibody. Ribosome marker. Suitable for IP, Dot, WB, IHC-P and reacts with Human, Synthetic peptide samples. Cited in 3 publications.
Images
![Immunoprecipitation - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (AB80158), expandable thumbnail](https://content.abcam.com/products/images/rps6-phospho-s235-antibody-ep13382y-ribosome-marker-ab80158--immunoprecipitation-img164284.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (AB80158)")
![Western blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (AB80158), expandable thumbnail](https://content.abcam.com/products/images/rps6-phospho-s235-antibody-ep13382y-ribosome-marker-ab80158--western-blot-img12333.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (AB80158)")
![Dot Blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (AB80158), expandable thumbnail](https://content.abcam.com/products/images/rps6-phospho-s235-antibody-ep13382y-ribosome-marker-ab80158--dot-blot-img993.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (AB80158)")
Publications
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- Oncology letters 27:182023AKR1B10 inhibits the proliferation and metastasis of hepatocellular carcinoma cells by regulating the PI3K/AKT pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKe Tian,Ying Deng,Zhipeng Li,Huaxin Zhou,Hui YaoPubMed 38034486
- Theranostics 11:10064-100732021Heterogeneity of cell composition and origin identified by single-cell transcriptomics in renal cysts of patients with autosomal dominant polycystic kidney disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiong Li,Yuchen Wang,Wenfeng Deng,Yanna Liu,Jian Geng,Ziyan Yan,Fei Li,Binshen Chen,Zhuolin Li,Renfei Xia,Wenli Zeng,Rumin Liu,Jian Xu,Fu Xiong,Chin-Lee Wu,Yun MiaoPubMed 34815804
- Stem cell research 11:1117-282013Differentiation of glutamatergic neurons from mouse embryonic stem cells requires raptor S6K signaling.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | Dot | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|---|
| Human | Tested | Not recommended | Expected | Tested | Not recommended | Tested |
| Synthetic peptide | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 - 1/200000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
Target data
Function
Component of the 40S small ribosomal subunit (PubMed:8706699). Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA (PubMed:17220279).
Alternative names
40S ribosomal protein S6, Phosphoprotein NP33, Small ribosomal subunit protein eS6, RPS6, OK/SW-cl.2
Recommended products
Rabbit Recombinant Monoclonal RPS6 phospho S235 antibody. Ribosome marker. Suitable for IP, Dot, WB, IHC-P and reacts with Human, Synthetic peptide samples. Cited in 3 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EP1338(2)Y
- Purity
Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RPS6 also known as Ribosomal Protein S6 is a component of the 40S ribosomal subunit with a molecular weight of approximately 28 kDa. This protein mainly localizes in the cytoplasm where ribosomes are fixed during protein synthesis. The ribosomal machinery uses RPS6 to translate mRNA into polypeptides operating as an essential part of protein biosynthesis. It expresses in various tissues with higher levels in highly proliferative cells which need extensive protein production for growth.
- Biological function summary
RPS6 is integral to the formation of the ribosomal complex. This complex is essential for the translation initiation process. RPS6 controls the translation capacity of ribosomes and functions in growth and development by regulating protein synthesis rates. Alterations in RPS6 activity can impact cellular growth and size due to its role in ribosome biogenesis.
- Pathways
RPS6 plays a significant role in the mTOR and PI3K/AKT signaling pathways. These pathways link nutrient availability to protein synthesis influencing cellular growth and metabolism. RPS6 interacts with proteins like S6K1 which is a downstream target in the mTOR pathway to modulate translation and cell growth processes.
- Associated diseases and disorders
RPS6 has connections to cancer and metabolic syndromes. Abnormal RPS6 activation can lead to dysregulated cell growth contributing to oncogenesis. In the context of cancer mTOR signaling where RPS6 functions is often upregulated leading to uncontrolled proliferation. Moreover in metabolic disorders the disrupted pathway activity involves other proteins like AKT fostering insulin resistance and metabolic imbalance.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunoprecipitation - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158), expandable thumbnail](https://content.abcam.com/products/images/rps6-phospho-s235-antibody-ep13382y-ribosome-marker-ab80158--immunoprecipitation-img164284.jpg "Immunoprecipitation - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)")
Immunoprecipitation - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)
RPS6 was immunoprecipitated from 0.35 mg 293T (Human embryonic kidney epithelial cell) treated with Calyculin A(100nM 30min) whole cell lysate 10 μg with ab80158 at 1/50 dilution (2μg). VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: 293T (Human embryonic kidney epithelial cell) treated with Calyculin A(100nM 30min) whole cell lysate 10 μg
Lane 2: ab80158 IP in 293T treated with Calyculin A(100nM 30min) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab80158 in 293T treated with Calyculin A(100nM 30min) whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)
Predicted band size: 29 kDa
Observed band size: 32 kDa
![Western blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158), expandable thumbnail](https://content.abcam.com/products/images/rps6-phospho-s235-antibody-ep13382y-ribosome-marker-ab80158--western-blot-img12333.jpg "Western blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)")
Western blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158) at 1/1000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) serum starved for 24 h. Whole cell lysates at 15 µg
Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) serum starved for 24 h, then 20% FBS incubated for 90 minutes. Whole cell lysates at 15 µg
Lane 3: HeLa (Human cervix adenocarcinoma epithelial cell) serum starved for 24 h, then 20% FBS incubated for 90 minutes. Whole cell lysates. Then the membrane was incubated with phosphatase. at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 30 kDa
Exposure time: 1min
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158), expandable thumbnail](https://content.abcam.com/products/images/rps6-phospho-s235-antibody-ep13382y-ribosome-marker-ab80158--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img76524.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)
ab80158 at 1/100-1/250 dilution staining RPS6 in human breast adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Dot Blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158), expandable thumbnail](https://content.abcam.com/products/images/rps6-phospho-s235-antibody-ep13382y-ribosome-marker-ab80158--dot-blot-img993.jpg "Dot Blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)")
Dot Blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)
Dot Blot analysis of -
Lane 1: Rb (pS235) phospho peptide
Lane 2: Rb (pS236) phospho peptide
Lane 3: Rb (pS235 & pS236) phospho peptide
Lane 4: Rb non-phospho peptide
5% NFDM/TBST was used as the diluting and blocking buffer.
Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.
![Western blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158), expandable thumbnail](https://content.abcam.com/products/images/rps6-phospho-s235-antibody-ep13382y-ribosome-marker-ab80158--western-blot-img87909.gif "Western blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)")
Western blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)
All lanes: Western blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158) at 1/200000 dilution
Lane 1: HeLa cell lysate, untreated at 10 µg
Lane 2: HeLa cell lysate, treated with Calyculin A at 10 µg
SecondaryAll lanes: goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 29 kDa
Observed band size: 30 kDa
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