AB80158

Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker

RabMAb
Recombinant
Lab Essentials
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(6 Publications)

Rabbit Recombinant Monoclonal RPS6 phospho S235 antibody. Ribosome marker. Suitable for IP, Dot, WB, IHC-P and reacts with Human, Synthetic peptide samples. Cited in 6 publications.

View Alternative Names

OK/SW-cl.2, RPS6, Small ribosomal subunit protein eS6, 40S ribosomal protein S6, Phosphoprotein NP33

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (AB80158)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (AB80158)

ab80158 at 1/100-1/250 dilution staining RPS6 in human breast adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Lab

Immunoprecipitation - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (AB80158)

RPS6 was immunoprecipitated from 0.35 mg 293T (Human embryonic kidney epithelial cell) treated with Calyculin A (100nM 30min) whole cell lysate 10 μg with ab80158 at 1/50 dilution (2μg). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : 293T (Human embryonic kidney epithelial cell) treated with Calyculin A (100nM 30min) whole cell lysate 10 μg

Lane 2 : ab80158 IP in 293T treated with Calyculin A (100nM 30min) whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab80158 in 293T treated with Calyculin A (100nM 30min) whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)

Predicted band size: 29 kDa

Observed band size: 32 kDa

false

Lab

Western blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (AB80158)

All lanes:

Western blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) serum starved for 24 h. Whole cell lysates at 15 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) serum starved for 24 h, then 20% FBS incubated for 90 minutes. Whole cell lysates at 15 µg

Lane 3:

HeLa (Human cervix adenocarcinoma epithelial cell) serum starved for 24 h, then 20% FBS incubated for 90 minutes. Whole cell lysates. Then the membrane was incubated with phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 29 kDa

Observed band size: 30 kDa

false

Exposure time: 1min

Unknown

Western blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (AB80158)

All lanes:

Western blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158) at 1/200000 dilution

Lane 1:

HeLa cell lysate, untreated at 10 µg

Lane 2:

HeLa cell lysate, treated with Calyculin A at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 29 kDa

Observed band size: 30 kDa

false

Unknown

Dot Blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (AB80158)

Dot Blot analysis of -

Lane 1 : Rb (pS235) phospho peptide

Lane 2 : Rb (pS236) phospho peptide

Lane 3 : Rb (pS235 & pS236) phospho peptide

Lane 4 : Rb non-phospho peptide

5% NFDM/TBST was used as the diluting and blocking buffer.

ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time : 3 minutes.

Carrier free

Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1338(2)Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, Dot, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/200000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPS6 also known as Ribosomal Protein S6 is a component of the 40S ribosomal subunit with a molecular weight of approximately 28 kDa. This protein mainly localizes in the cytoplasm where ribosomes are fixed during protein synthesis. The ribosomal machinery uses RPS6 to translate mRNA into polypeptides operating as an essential part of protein biosynthesis. It expresses in various tissues with higher levels in highly proliferative cells which need extensive protein production for growth.

Biological function summary

RPS6 is integral to the formation of the ribosomal complex. This complex is essential for the translation initiation process. RPS6 controls the translation capacity of ribosomes and functions in growth and development by regulating protein synthesis rates. Alterations in RPS6 activity can impact cellular growth and size due to its role in ribosome biogenesis.

Pathways

RPS6 plays a significant role in the mTOR and PI3K/AKT signaling pathways. These pathways link nutrient availability to protein synthesis influencing cellular growth and metabolism. RPS6 interacts with proteins like S6K1 which is a downstream target in the mTOR pathway to modulate translation and cell growth processes.

RPS6 has connections to cancer and metabolic syndromes. Abnormal RPS6 activation can lead to dysregulated cell growth contributing to oncogenesis. In the context of cancer mTOR signaling where RPS6 functions is often upregulated leading to uncontrolled proliferation. Moreover in metabolic disorders the disrupted pathway activity involves other proteins like AKT fostering insulin resistance and metabolic imbalance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the 40S small ribosomal subunit (PubMed : 23636399, PubMed : 8706699). Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA (PubMed : 17220279). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).

See full target information RPS6 pS235

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:527 PubMed40346630

2025

ESPN activates ZEB1-mediated EMT through the PI3K/AKT/mTOR axis to promote osteosarcoma metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Ruikai Zhou,Hongyu Wu,Hao You,Xiaofei Wang,Xiuchen Yuan,Zhengyi Sun,Dong Zhou,Yuqing Jiang,Yifei Shen

iScience 27:109172 PubMed38414864

2024

Disruption of mitochondrial energy metabolism is a putative pathogenesis of Diamond-Blackfan anemia.

Applications

Unspecified application

Species

Unspecified reactive species

Rudan Xiao,Lijuan Zhang,Zijuan Xin,Junwei Zhu,Qian Zhang,Guangmin Zheng,Siyun Chu,Jing Wu,Lu Zhang,Yang Wan,Xiaojuan Chen,Weiping Yuan,Zhaojun Zhang,Xiaofan Zhu,Xiangdong Fang

Oncology letters 27:18 PubMed38034486

2023

AKR1B10 inhibits the proliferation and metastasis of hepatocellular carcinoma cells by regulating the PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Tian,Ying Deng,Zhipeng Li,Huaxin Zhou,Hui Yao

Clinical and translational medicine 11:e674 PubMed34954904

2021

Biological significance of MYC and CEBPD coamplification in urothelial carcinoma: Multilayered genomic, transcriptional and posttranscriptional positive feedback loops enhance oncogenic glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Ti-Chun Chan,Yi-Ting Chen,Kien Thiam Tan,Chia-Ling Wu,Wen-Jeng Wu,Wei-Ming Li,Ju-Ming Wang,Yow-Ling Shiue,Chien-Feng Li

Theranostics 11:10064-10073 PubMed34815804

2021

Heterogeneity of cell composition and origin identified by single-cell transcriptomics in renal cysts of patients with autosomal dominant polycystic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Li,Yuchen Wang,Wenfeng Deng,Yanna Liu,Jian Geng,Ziyan Yan,Fei Li,Binshen Chen,Zhuolin Li,Renfei Xia,Wenli Zeng,Rumin Liu,Jian Xu,Fu Xiong,Chin-Lee Wu,Yun Miao

Stem cell research 11:1117-28 PubMed23988668

2013

Differentiation of glutamatergic neurons from mouse embryonic stem cells requires raptor S6K signaling.

Applications

Unspecified application

Species

Mouse

Jen-Hua Chuang,Li-Chu Tung,Yi Yin,Yenshou Lin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (AB80158)

Immunoprecipitation - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (AB80158)

Western blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (AB80158)

Western blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (AB80158)

Dot Blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (AB80158)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Properties and storage information

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Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise