AB12864

Anti-RPS6 (phospho S235 + S236) antibody

Name: Anti-RPS6 (phospho S235 + S236) antibody (ab12864) | Abcam
Brand: Abcam Limited
SKU: AB12864
Price: 400 USD
Availability: InStock
Rating: 5 (4 reviews)

Lab Essentials
What is this?

(4 Reviews)

(13 Publications)

Rabbit Polyclonal RPS6 phospho S235 + S236 antibody. Suitable for WB and reacts with Human samples. Cited in 13 publications.

View Alternative Names

OK/SW-cl.2, RPS6, Small ribosomal subunit protein eS6, 40S ribosomal protein S6, Phosphoprotein NP33

1 Images

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Western blot - Anti-RPS6 (phospho S235 + S236) antibody (AB12864)

Peptide Competition.
Lysates prepared from HeLa cells left untreated (1) or treated with anisomycin (2-5) were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ab12864 for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with : no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with a goat anti-rabbit IgG HRP conjugate secondary antibody and bands were detected using the Pierce SuperSignal^TM method. The data show that only the peptide corresponding to RPS6 blocks the signal, verifying the specificity of the antibody. Peptide Competition. Lysates prepared from HeLa cells left untreated (1) or treated with anisomycin (2-5) were resolved by SDS-PAGE on a 14% po

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Western blot - Anti-RPS6 (phospho S235 + S236) antibody (ab12864)

Predicted band size: 29 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody does not cross react with RPS6 phosphorylated on serines 244 and 247.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated RPS6. The final product is generated by affinity chromatography using a RPS6-derived peptide that is phosphorylated at serines 235 and 236.

Storage buffer

pH: 7.3 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPS6 also known as Ribosomal Protein S6 is a component of the 40S ribosomal subunit with a molecular weight of approximately 28 kDa. This protein mainly localizes in the cytoplasm where ribosomes are fixed during protein synthesis. The ribosomal machinery uses RPS6 to translate mRNA into polypeptides operating as an essential part of protein biosynthesis. It expresses in various tissues with higher levels in highly proliferative cells which need extensive protein production for growth.

Biological function summary

RPS6 is integral to the formation of the ribosomal complex. This complex is essential for the translation initiation process. RPS6 controls the translation capacity of ribosomes and functions in growth and development by regulating protein synthesis rates. Alterations in RPS6 activity can impact cellular growth and size due to its role in ribosome biogenesis.

Pathways

RPS6 plays a significant role in the mTOR and PI3K/AKT signaling pathways. These pathways link nutrient availability to protein synthesis influencing cellular growth and metabolism. RPS6 interacts with proteins like S6K1 which is a downstream target in the mTOR pathway to modulate translation and cell growth processes.

RPS6 has connections to cancer and metabolic syndromes. Abnormal RPS6 activation can lead to dysregulated cell growth contributing to oncogenesis. In the context of cancer mTOR signaling where RPS6 functions is often upregulated leading to uncontrolled proliferation. Moreover in metabolic disorders the disrupted pathway activity involves other proteins like AKT fostering insulin resistance and metabolic imbalance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the 40S small ribosomal subunit (PubMed : 23636399, PubMed : 8706699). Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA (PubMed : 17220279). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).

See full target information RPS6 phospho S235 + S236

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 9:382 PubMed37852974

2023

KCNAB2 overexpression inhibits human non-small-cell lung cancer cell growth in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Cheng,Yu-Fei Tang,Yang Cao,Shi-Qing Peng,Xiao-Ren Zhu,Yue Sun,Shu-Hang Wang,Bin Wang,Yi-Min Lu

FEBS open bio 12:71-81 PubMed34431239

2021

AAV expressing an mTOR-inhibiting siRNA exhibits therapeutic potential in retinal vascular disorders by preserving endothelial integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Seho Cha,Won-Il Seo,Ha-Na Woo,Hee Jong Kim,Steven Hyun Seung Lee,Jin Kim,Jun-Sub Choi,Keerang Park,Joo Yong Lee,Beom Jun Lee,Heuiran Lee

Frontiers in oncology 11:644592 PubMed34178631

2021

Normalization of Enzyme Expression and Activity Regulating Vitamin A Metabolism Increases RAR-Beta Expression and Reduces Cellular Migration and Proliferation in Diseases Caused by Tuberous Sclerosis Gene Mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Elhusseiny Mohamed Mahmoud Abdelwahab,Judit Bovari-Biri,Gabor Smuk,Tunde Harko,Janos Fillinger,Judit Moldvay,Vera P Krymskaya,Judit E Pongracz

Theranostics 10:6915-6927 PubMed32550912

2020

RSK-3 promotes cartilage regeneration via interacting with rpS6 in cartilage stem/progenitor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Zhang,Md Rana Hamid,Ting Wang,Jinqi Liao,Liru Wen,Yan Zhou,Pengfei Wei,Xuenong Zou,Gang Chen,Junhui Chen,Guangqian Zhou

BioMed research international 2020:9121894 PubMed32071922

2020

Hydrogen-Rich Saline Inhibits Lipopolysaccharide-Induced Acute Lung Injury and Endothelial Dysfunction by Regulating Autophagy through mTOR/TFEB Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiling Fu,Ze Zhang,Xiuying Wu,Jin Zhang

Journal of dairy science 102:4264-4274 PubMed30879806

2019

Phosphorylation of AKT serine/threonine kinase and abundance of milk protein synthesis gene networks in mammary tissue in response to supply of methionine in periparturient Holstein cows.

Applications

Unspecified application

Species

Unspecified reactive species

Y F Ma,F Batistel,T L Xu,L Q Han,R Bucktrout,Y Liang,D N Coleman,C Parys,J J Loor

Molecular carcinogenesis 56:2317-2331 PubMed28574600

2017

Diallyl trisulfides, a natural histone deacetylase inhibitor, attenuate HIF-1α synthesis, and decreases breast cancer metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhonghong Wei,Yunlong Shan,Li Tao,Yuping Liu,Zhijie Zhu,Zhaoguo Liu,Yuanyuan Wu,Wenxing Chen,Aiyun Wang,Yin Lu

Journal of cellular and molecular medicine 20:1908-19 PubMed27385290

2016

Danshensu alleviates cardiac ischaemia/reperfusion injury by inhibiting autophagy and apoptosis via activation of mTOR signalling.

Applications

Unspecified application

Species

Rat

Guanwei Fan,Jiahui Yu,Patrick Fordjour Asare,Lingyan Wang,Han Zhang,Boli Zhang,Yan Zhu,Xiumei Gao

Cancer discovery 5:255-63 PubMed25564516

2015

Lysine demethylase KDM4A associates with translation machinery and regulates protein synthesis.

Applications

Unspecified application

Species

Human

Capucine Van Rechem,Joshua C Black,Myriam Boukhali,Martin J Aryee,Susanne Gräslund,Wilhelm Haas,Cyril H Benes,Johnathan R Whetstine

Human molecular genetics 20:2322-32 PubMed21487022

2011

Mutations in PNKD causing paroxysmal dyskinesia alters protein cleavage and stability.

Applications

ICC/IF

Species

Human

Yiguo Shen,Hsien-Yang Lee,Joel Rawson,Sunil Ojha,Patricia Babbitt,Ying-Hui Fu,Louis J Ptácek

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-RPS6 (phospho S235 + S236) antibody

Western blot - Anti-RPS6 (phospho S235 + S236) antibody (AB12864)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

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