Sheep Polyclonal RPS6 phospho S235 + S236 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human RPS6 phospho S235 + S236 aa 200 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
Images
Key facts
- Isotype
IgG
- Host species
Sheep
- Storage buffer
pH: 7.5
Preservative: 0.05% Sodium azide
Constituents: 0.87% Sodium chloride, 0.238% HEPES- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human RPS6 phospho S235 + S236 aa 200 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P62753
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Rat | Predicted |
| Chicken | Predicted |
| Cow | Predicted |
| Cynomolgus monkey | Predicted |
| Xenopus laevis | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Cow, Xenopus laevis, Cynomolgus monkey | Dilution info - | Notes - |
Associated Products
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Target data
Function
Component of the 40S small ribosomal subunit (PubMed:8706699). Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA (PubMed:17220279).
Alternative names
40S ribosomal protein S6, Phosphoprotein NP33, Small ribosomal subunit protein eS6, OK/SW-cl.2, RPS6
Recommended products
Sheep Polyclonal RPS6 phospho S235 + S236 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human RPS6 phospho S235 + S236 aa 200 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
Alternative names
40S ribosomal protein S6, Phosphoprotein NP33, Small ribosomal subunit protein eS6, OK/SW-cl.2, RPS6
Key facts
- Isotype
IgG
- Host species
Sheep
- Storage buffer
pH: 7.5
Preservative: 0.05% Sodium azide
Constituents: 0.87% Sodium chloride, 0.238% HEPES- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human RPS6 phospho S235 + S236 aa 200 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P62753
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RPS6 also known as Ribosomal Protein S6 is a component of the 40S ribosomal subunit with a molecular weight of approximately 28 kDa. This protein mainly localizes in the cytoplasm where ribosomes are fixed during protein synthesis. The ribosomal machinery uses RPS6 to translate mRNA into polypeptides operating as an essential part of protein biosynthesis. It expresses in various tissues with higher levels in highly proliferative cells which need extensive protein production for growth.
- Biological function summary
RPS6 is integral to the formation of the ribosomal complex. This complex is essential for the translation initiation process. RPS6 controls the translation capacity of ribosomes and functions in growth and development by regulating protein synthesis rates. Alterations in RPS6 activity can impact cellular growth and size due to its role in ribosome biogenesis.
- Pathways
RPS6 plays a significant role in the mTOR and PI3K/AKT signaling pathways. These pathways link nutrient availability to protein synthesis influencing cellular growth and metabolism. RPS6 interacts with proteins like S6K1 which is a downstream target in the mTOR pathway to modulate translation and cell growth processes.
- Associated diseases and disorders
RPS6 has connections to cancer and metabolic syndromes. Abnormal RPS6 activation can lead to dysregulated cell growth contributing to oncogenesis. In the context of cancer mTOR signaling where RPS6 functions is often upregulated leading to uncontrolled proliferation. Moreover in metabolic disorders the disrupted pathway activity involves other proteins like AKT fostering insulin resistance and metabolic imbalance.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-RPS6 (phospho S235 + S236) antibody (ab65750)
All lanes: Western blot - Anti-RPS6 (phospho S235 + S236) antibody (ab65750) at 2 µg/mL
Lane 1: Raji cell extracts, untreated
Lane 2: Raji cell extracts, treated with 10 µg/ml S6 inhibitor for 30 min
Predicted band size: 29 kDa
Observed band size: 30 kDa, 48 kDa
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