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Rabbit Recombinant Monoclonal RPS6 phospho S235 + S236 antibody. Carrier free. Suitable for IHC-P, Flow Cyt (Intra), Dot, ICC/IF and reacts with Mouse, Human, Synthetic peptide samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (AB236224), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (AB236224), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (AB236224), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (AB236224), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (AB236224), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow Cyt (Intra)DotICC/IF
Human
Tested
Tested
Expected
Tested
Mouse
Tested
Expected
Expected
Expected
Synthetic peptide
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Component of the 40S small ribosomal subunit (PubMed:8706699). Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA (PubMed:17220279).

Alternative names

40S ribosomal protein S6, Phosphoprotein NP33, Small ribosomal subunit protein eS6, OK/SW-cl.2, RPS6

Recommended products

Rabbit Recombinant Monoclonal RPS6 phospho S235 + S236 antibody. Carrier free. Suitable for IHC-P, Flow Cyt (Intra), Dot, ICC/IF and reacts with Mouse, Human, Synthetic peptide samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

SP50

Purification technique

Affinity purification Protein A/G

Concentration
Loading...
Purification notes

Purified from TCS by protein A/G.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab236224 is the carrier-free version of Anti-RPS6 (phospho S235 + S236) antibody [SP50] ab101691.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

RPS6 also known as Ribosomal Protein S6 is a component of the 40S ribosomal subunit with a molecular weight of approximately 28 kDa. This protein mainly localizes in the cytoplasm where ribosomes are fixed during protein synthesis. The ribosomal machinery uses RPS6 to translate mRNA into polypeptides operating as an essential part of protein biosynthesis. It expresses in various tissues with higher levels in highly proliferative cells which need extensive protein production for growth.

Biological function summary

RPS6 is integral to the formation of the ribosomal complex. This complex is essential for the translation initiation process. RPS6 controls the translation capacity of ribosomes and functions in growth and development by regulating protein synthesis rates. Alterations in RPS6 activity can impact cellular growth and size due to its role in ribosome biogenesis.

Pathways

RPS6 plays a significant role in the mTOR and PI3K/AKT signaling pathways. These pathways link nutrient availability to protein synthesis influencing cellular growth and metabolism. RPS6 interacts with proteins like S6K1 which is a downstream target in the mTOR pathway to modulate translation and cell growth processes.

Associated diseases and disorders

RPS6 has connections to cancer and metabolic syndromes. Abnormal RPS6 activation can lead to dysregulated cell growth contributing to oncogenesis. In the context of cancer mTOR signaling where RPS6 functions is often upregulated leading to uncontrolled proliferation. Moreover in metabolic disorders the disrupted pathway activity involves other proteins like AKT fostering insulin resistance and metabolic imbalance.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling RPS6 with Anti-RPS6 (phospho S235 + S236) antibody [SP50] ab101691 at 1/100 dilution (1.23 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Dako™REAL™ EnVision™ Detection System (K5007) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining on human rat spleen without alkaline phosphatase treatment (image A); and no signal was detected when tissues were treated with alkaline phosphatase (image B), performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with Anti-RPS6 (phospho S235 + S236) antibody [SP50] ab101691 for 10 mins at room temperature. This image was generated using Anti-RPS6 (phospho S235 + S236) antibody [SP50] ab101691, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse stomach tissue sections labeling RPS6 with Anti-RPS6 (phospho S235 + S236) antibody [SP50] ab101691 at 1/100 dilution (1.23 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Dako™REAL™ EnVision™ Detection System (K5007) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining on mouse stomach without alkaline phosphatase treatment (image A); and no signal was detected when tissues were treated with alkaline phosphatase (image B), performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with Anti-RPS6 (phospho S235 + S236) antibody [SP50] ab101691 for 10 mins at room temperature. This image was generated using Anti-RPS6 (phospho S235 + S236) antibody [SP50] ab101691, the same clone, but with a different buffer formulation.

  • Flow Cytometry (Intracellular) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224)

    Flow Cytometry analysis of PC-3 (Human prostate adenocarcinoma epithelial cell) cells labeling RPS6 with purified Anti-RPS6 (phospho S235 + S236) antibody [SP50] ab101691 at 1:200 dilution (0.615 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD10 antibody [SP67] ab227640).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling RPS6 with Anti-RPS6 (phospho S235 + S236) antibody [SP50] ab101691 at 1/100 dilution (1.23 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Dako™ REAL™ EnVision™ Detection System (K5007) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining on human breast carcinoma without alkaline phosphatase treatment (image A); and no signal was detected when tissues were treated with alkaline phosphatase (image B), performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with Anti-RPS6 (phospho S235 + S236) antibody [SP50] ab101691 for 10 mins at room temperature. This image was generated using Anti-RPS6 (phospho S235 + S236) antibody [SP50] ab101691, the same clone, but with a different buffer formulation.

  • Immunocytochemistry/ Immunofluorescence - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224)

    Immunocytochemistry/ Immunofluorescence analysis of PC-3 (human prostate adenocarcinoma epithelial cell) treated with Lamda phosphatase cells labeling RPS6 with purified Anti-RPS6 (phospho S235 + S236) antibody [SP50] ab101691 at 1:500 (0.25 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-EGFR antibody [SP84] - C-terminal ab227642).

  • Dot Blot - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224), expandable thumbnail

    Dot Blot - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224)

    Dot blot analysis of RPS6 (phospho S235 + S236) peptide (Lane 1), RPS6 (phospho S235) peptide (phospho 2) RPS6 (phospho S236) peptide (Lane 3) and RPS6 non-phospho peptide (Lane 4) labeling RPS6 (phospho S235 + S236) peptide with purified Anti-RPS6 (phospho S235 + S236) antibody [SP50] ab101691 at 0.123 ug/mL. Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 0.01 ug/mL.

    Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RPS6 (phospho S235 + S236) antibody [SP50] ab101691).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224)

    Anti-RPS6 (phospho S235 + S236) antibody [SP50] ab101691 at 1/100 dilution staining RPS6 (phospho S235 + S236) in human breast carcinoma by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-RPS6 (phospho S235 + S236) antibody [SP50] ab101691).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com