Anti-RPS6 (phospho S235 + S236) [SP50] antibody (ab236224) is a rabbit monoclonal antibody that is provided in a PBS only formulation free from BSA and azide. Formulated to be conjugation-ready. It is used to detect RPS6 in Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF, Dot Blot. Suitable for Human, Mouse samples.
- BSA, sodium azide, and glycerol-free for consistent conjugation with fluorochromes, enzymes and more
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (AB236224), expandable thumbnail](https://content.abcam.com/products/images/rps6-phospho-s235-s236-antibody-sp50-bsa-and-azide-free-ab236224--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134479.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (AB236224)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (AB236224), expandable thumbnail](https://content.abcam.com/products/images/rps6-phospho-s235-s236-antibody-sp50-bsa-and-azide-free-ab236224--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117307.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (AB236224)")
![Flow Cytometry (Intracellular) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (AB236224), expandable thumbnail](https://content.abcam.com/products/images/rps6-phospho-s235-s236-antibody-sp50-bsa-and-azide-free-ab236224--flow-cytometry-intracellular-img78754.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (AB236224)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (AB236224), expandable thumbnail](https://content.abcam.com/products/images/rps6-phospho-s235-s236-antibody-sp50-bsa-and-azide-free-ab236224--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135835.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (AB236224)")
![Immunocytochemistry/ Immunofluorescence - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (AB236224), expandable thumbnail](https://content.abcam.com/products/images/rps6-phospho-s235-s236-antibody-sp50-bsa-and-azide-free-ab236224--immunocytochemistry-immunofluorescence-img128360.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (AB236224)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | Flow Cyt (Intra) | Dot | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Expected | Tested |
| Mouse | Tested | Expected | Expected | Expected |
| Synthetic peptide | Not recommended | Not recommended | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
Target data
Function
Component of the 40S small ribosomal subunit (PubMed:23636399, PubMed:8706699). Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA (PubMed:17220279). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797).
Alternative names
OK/SW-cl.2, RPS6, Small ribosomal subunit protein eS6, 40S ribosomal protein S6, Phosphoprotein NP33
Recommended products
Anti-RPS6 (phospho S235 + S236) [SP50] antibody (ab236224) is a rabbit monoclonal antibody that is provided in a PBS only formulation free from BSA and azide. Formulated to be conjugation-ready. It is used to detect RPS6 in Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF, Dot Blot. Suitable for Human, Mouse samples.
- BSA, sodium azide, and glycerol-free for consistent conjugation with fluorochromes, enzymes and more
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogens
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- SP50
- Purification technique
- Affinity purification Protein A/G
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224), expandable thumbnail](https://content.abcam.com/products/images/rps6-phospho-s235-s236-antibody-sp50-bsa-and-azide-free-ab236224--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134479.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling RPS6 with Anti-RPS6 (phospho S235 + S236) antibody [SP50] ab101691 at 1/100 dilution (1.23 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Dako™REAL™ EnVision™ Detection System (K5007) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining on human rat spleen without alkaline phosphatase treatment (image A); and no signal was detected when tissues were treated with alkaline phosphatase (image B), performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with Anti-RPS6 (phospho S235 + S236) antibody [SP50] ab101691 for 10 mins at room temperature. This image was generated using Anti-RPS6 (phospho S235 + S236) antibody [SP50] ab101691, the same clone, but with a different buffer formulation.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224), expandable thumbnail](https://content.abcam.com/products/images/rps6-phospho-s235-s236-antibody-sp50-bsa-and-azide-free-ab236224--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117307.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse stomach tissue sections labeling RPS6 with Anti-RPS6 (phospho S235 + S236) antibody [SP50] ab101691 at 1/100 dilution (1.23 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Dako™REAL™ EnVision™ Detection System (K5007) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining on mouse stomach without alkaline phosphatase treatment (image A); and no signal was detected when tissues were treated with alkaline phosphatase (image B), performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with Anti-RPS6 (phospho S235 + S236) antibody [SP50] ab101691 for 10 mins at room temperature. This image was generated using Anti-RPS6 (phospho S235 + S236) antibody [SP50] ab101691, the same clone, but with a different buffer formulation.![Flow Cytometry (Intracellular) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224), expandable thumbnail](https://content.abcam.com/products/images/rps6-phospho-s235-s236-antibody-sp50-bsa-and-azide-free-ab236224--flow-cytometry-intracellular-img78754.jpg "Flow Cytometry (Intracellular) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224)")
Flow Cytometry (Intracellular) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224)
Flow Cytometry analysis of PC-3 (Human prostate adenocarcinoma epithelial cell) cells labeling RPS6 with purified Anti-RPS6 (phospho S235 + S236) antibody [SP50] ab101691 at 1:200 dilution (0.615 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD10 antibody [SP67] ab227640).![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224), expandable thumbnail](https://content.abcam.com/products/images/rps6-phospho-s235-s236-antibody-sp50-bsa-and-azide-free-ab236224--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135835.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling RPS6 with Anti-RPS6 (phospho S235 + S236) antibody [SP50] ab101691 at 1/100 dilution (1.23 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Dako™ REAL™ EnVision™ Detection System (K5007) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining on human breast carcinoma without alkaline phosphatase treatment (image A); and no signal was detected when tissues were treated with alkaline phosphatase (image B), performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with Anti-RPS6 (phospho S235 + S236) antibody [SP50] ab101691 for 10 mins at room temperature. This image was generated using Anti-RPS6 (phospho S235 + S236) antibody [SP50] ab101691, the same clone, but with a different buffer formulation.![Immunocytochemistry/ Immunofluorescence - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224), expandable thumbnail](https://content.abcam.com/products/images/rps6-phospho-s235-s236-antibody-sp50-bsa-and-azide-free-ab236224--immunocytochemistry-immunofluorescence-img128360.jpg "Immunocytochemistry/ Immunofluorescence - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224)")
Immunocytochemistry/ Immunofluorescence - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224)
Immunocytochemistry/ Immunofluorescence analysis of PC-3 (human prostate adenocarcinoma epithelial cell) treated with Lamda phosphatase cells labeling RPS6 with purified Anti-RPS6 (phospho S235 + S236) antibody [SP50] ab101691 at 1:500 (0.25 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-EGFR antibody [SP84] - C-terminal ab227642).![Dot Blot - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224), expandable thumbnail](https://content.abcam.com/products/images/rps6-phospho-s235-s236-antibody-sp50-bsa-and-azide-free-ab236224--dot-blot-img2981.jpg "Dot Blot - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224)")
Dot Blot - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224)
Dot blot analysis of RPS6 (phospho S235 + S236) peptide (Lane 1), RPS6 (phospho S235) peptide (phospho 2) RPS6 (phospho S236) peptide (Lane 3) and RPS6 non-phospho peptide (Lane 4) labeling RPS6 (phospho S235 + S236) peptide with purified Anti-RPS6 (phospho S235 + S236) antibody [SP50] ab101691 at 0.123 ug/mL. Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 0.01 ug/mL.
Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RPS6 (phospho S235 + S236) antibody [SP50] ab101691).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224), expandable thumbnail](https://content.abcam.com/products/images/rps6-phospho-s235-s236-antibody-sp50-bsa-and-azide-free-ab236224--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img93437.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224)
Anti-RPS6 (phospho S235 + S236) antibody [SP50] ab101691 at 1/100 dilution staining RPS6 (phospho S235 + S236) in human breast carcinoma by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-RPS6 (phospho S235 + S236) antibody [SP50] ab101691).
![Dot Blot - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224), expandable thumbnail](https://content.abcam.com/products/images/rps6-phospho-s235-s236-antibody-sp50-bsa-and-azide-free-ab236224--dot-blot-img2981.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224), expandable thumbnail](https://content.abcam.com/products/images/rps6-phospho-s235-s236-antibody-sp50-bsa-and-azide-free-ab236224--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img93437.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] - BSA and Azide free (ab236224)")
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