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Rabbit Recombinant Monoclonal RPS6 phospho S240 + S244 antibody. Suitable for IHC-P, Flow Cyt (Intra), ELISA, Dot, ICC/IF and reacts with Human, Synthetic peptide samples.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S240 + S244) antibody [SP45] (AB81081), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-RPS6 (phospho S240 + S244) antibody [SP45] (AB81081), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-RPS6 (phospho S240 + S244) antibody [SP45] (AB81081), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S240 + S244) antibody [SP45] (AB81081), expandable thumbnail
  • Dot Blot - Anti-RPS6 (phospho S240 + S244) antibody [SP45] (AB81081), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow Cyt (Intra)ELISADotICC/IF
Human
Tested
Tested
Expected
Expected
Tested
Mouse
Predicted
Predicted
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Predicted
Predicted
Chicken
Predicted
Predicted
Predicted
Predicted
Predicted
Cow
Predicted
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Predicted
Pig
Predicted
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Predicted
Synthetic peptide
Not recommended
Not recommended
Tested
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/50
Notes

Primary antibody incubation for 30 minutes at room temperature.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Pig, Mouse, Rat, Rabbit, Chicken, Cow, Dog
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/640
Notes

-

Predicted
Predicted

Species
Pig, Mouse, Rat, Rabbit, Chicken, Cow, Dog
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
1 µg/mL
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Pig, Mouse, Rat, Rabbit, Chicken, Cow, Dog
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
0.643 µg/mL
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Pig, Mouse, Rat, Rabbit, Chicken, Cow, Dog
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Predicted
Predicted

Species
Pig, Mouse, Rat, Rabbit, Chicken, Cow, Dog
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Component of the 40S small ribosomal subunit (PubMed:23636399, PubMed:8706699). Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA (PubMed:17220279). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797).

Alternative names

OK/SW-cl.2, RPS6, Small ribosomal subunit protein eS6, 40S ribosomal protein S6, Phosphoprotein NP33

Recommended products

Rabbit Recombinant Monoclonal RPS6 phospho S240 + S244 antibody. Suitable for IHC-P, Flow Cyt (Intra), ELISA, Dot, ICC/IF and reacts with Human, Synthetic peptide samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
SP45
Purification technique
Affinity purification Protein A/G
Concentration
Loading...
Purification notes

Purified from TCS by protein A/G.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

RPS6 also known as Ribosomal Protein S6 is a component of the 40S ribosomal subunit with a molecular weight of approximately 28 kDa. This protein mainly localizes in the cytoplasm where ribosomes are fixed during protein synthesis. The ribosomal machinery uses RPS6 to translate mRNA into polypeptides operating as an essential part of protein biosynthesis. It expresses in various tissues with higher levels in highly proliferative cells which need extensive protein production for growth.

Biological function summary

RPS6 is integral to the formation of the ribosomal complex. This complex is essential for the translation initiation process. RPS6 controls the translation capacity of ribosomes and functions in growth and development by regulating protein synthesis rates. Alterations in RPS6 activity can impact cellular growth and size due to its role in ribosome biogenesis.

Pathways

RPS6 plays a significant role in the mTOR and PI3K/AKT signaling pathways. These pathways link nutrient availability to protein synthesis influencing cellular growth and metabolism. RPS6 interacts with proteins like S6K1 which is a downstream target in the mTOR pathway to modulate translation and cell growth processes.

Associated diseases and disorders

RPS6 has connections to cancer and metabolic syndromes. Abnormal RPS6 activation can lead to dysregulated cell growth contributing to oncogenesis. In the context of cancer mTOR signaling where RPS6 functions is often upregulated leading to uncontrolled proliferation. Moreover in metabolic disorders the disrupted pathway activity involves other proteins like AKT fostering insulin resistance and metabolic imbalance.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S240 + S244) antibody [SP45] (ab81081), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S240 + S244) antibody [SP45] (ab81081)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling RPS6 with ab81081 at 1/50 dilution (3.86 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining on human breast carcinoma without lambda phosphatase treatment (image A); no signal can be observed after treatment with lambda phosphatase (image B), performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab81082 for 30 mins at room temperature.

  • Immunocytochemistry/ Immunofluorescence - Anti-RPS6 (phospho S240 + S244) antibody [SP45] (ab81081), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RPS6 (phospho S240 + S244) antibody [SP45] (ab81081)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) treated with lamda phosphatase cells labeling RPS6 with purified ab81081 at 1/500 (1.3 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry (Intracellular) - Anti-RPS6 (phospho S240 + S244) antibody [SP45] (ab81081), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-RPS6 (phospho S240 + S244) antibody [SP45] (ab81081)

    Flow cytometry analysis of MCF7 grown in serum-free media overnight, then grown in 20% FBS media for 30 minutes labeling RPS6 with purified ab81081 at 1/640 dilution (1.00 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black. Unlableled control -Unlabelled cells / blue.Untreated cells (Green).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S240 + S244) antibody [SP45] (ab81081), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S240 + S244) antibody [SP45] (ab81081)

    Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for RPS6 (phospho S240 + S244) using ab81081 at 1/50 dilution in immunohistochemical analysis.

  • Dot Blot - Anti-RPS6 (phospho S240 + S244) antibody [SP45] (ab81081), expandable thumbnail

    Dot Blot - Anti-RPS6 (phospho S240 + S244) antibody [SP45] (ab81081)

    Dot blot analysis of RPS6 (phospho S240 + S244) peptide (Lane 1), RPS6 (phospho S240) peptide (Lane 2), RPS6 (phospho S244) peptide (Lane 3) and RPS6 non-phospho peptide (Lane 4) RPS6 labeling (phospho S240 + S244) with purified ab81081 at 0.643 μg/mL. Goat Anti-Rabbit IgG H&L (HRP) ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at 0.01 μg/mL.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • ELISA - Anti-RPS6 (phospho S240 + S244) antibody [SP45] (ab81081), expandable thumbnail

    ELISA - Anti-RPS6 (phospho S240 + S244) antibody [SP45] (ab81081)

    ELISA antigen dose-response curve using purified ab81081 at 0.1-1000 ng/mL. Antigen concentration of 0-10000 ng/mL. A Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) (Jackson ImmunoResearch) (1/2500) was used as the secondary antibody.

    RPS (phospho S240 + S244): RA-S(p)-TSK-S(p)-ESSQKC
    RPS (phospho S240) : RA-S(p)-TSKSESSQKC
    RPS (phospho S244): RASTSK-S(p)-ESSQKC
    RPS non-phospho: RASTSKSESSQKC

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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