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Rabbit Recombinant Monoclonal RPS6 phospho S240 + S244 antibody. Carrier free. Suitable for IHC-P, ELISA, Dot, ICC/IF, Flow Cyt (Intra) and reacts with Human, Synthetic peptide samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S240 + S244) antibody [SP45] - BSA and Azide free (AB236438), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S240 + S244) antibody [SP45] - BSA and Azide free (AB236438), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-RPS6 (phospho S240 + S244) antibody [SP45] - BSA and Azide free (AB236438), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-RPS6 (phospho S240 + S244) antibody [SP45] - BSA and Azide free (AB236438), expandable thumbnail
  • Dot Blot - Anti-RPS6 (phospho S240 + S244) antibody [SP45] - BSA and Azide free (AB236438), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PELISADotICC/IFFlow Cyt (Intra)
Human
Tested
Expected
Expected
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Predicted
Predicted
Chicken
Predicted
Predicted
Predicted
Predicted
Predicted
Cow
Predicted
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Predicted
Pig
Predicted
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Predicted
Synthetic peptide
Not recommended
Tested
Tested
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

-

Notes

Primary antibody incubation for 30 minutes at room temperature.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat, Rabbit, Chicken, Cow, Dog, Pig

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Rabbit, Chicken, Cow, Dog, Pig

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Rabbit, Chicken, Cow, Dog, Pig

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Rabbit, Chicken, Cow, Dog, Pig

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Rabbit, Chicken, Cow, Dog, Pig

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Component of the 40S small ribosomal subunit (PubMed:8706699). Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA (PubMed:17220279).

Alternative names

40S ribosomal protein S6, Phosphoprotein NP33, Small ribosomal subunit protein eS6, RPS6, OK/SW-cl.2

Recommended products

Rabbit Recombinant Monoclonal RPS6 phospho S240 + S244 antibody. Carrier free. Suitable for IHC-P, ELISA, Dot, ICC/IF, Flow Cyt (Intra) and reacts with Human, Synthetic peptide samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

SP45

Purification technique

Affinity purification Protein A/G

Concentration
Loading...
Purification notes

Purified from TCS by protein A/G.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab236438 is the carrier-free version of Anti-RPS6 (phospho S240 + S244) antibody [SP45] ab81081.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

RPS6 also known as Ribosomal Protein S6 is a component of the 40S ribosomal subunit with a molecular weight of approximately 28 kDa. This protein mainly localizes in the cytoplasm where ribosomes are fixed during protein synthesis. The ribosomal machinery uses RPS6 to translate mRNA into polypeptides operating as an essential part of protein biosynthesis. It expresses in various tissues with higher levels in highly proliferative cells which need extensive protein production for growth.

Biological function summary

RPS6 is integral to the formation of the ribosomal complex. This complex is essential for the translation initiation process. RPS6 controls the translation capacity of ribosomes and functions in growth and development by regulating protein synthesis rates. Alterations in RPS6 activity can impact cellular growth and size due to its role in ribosome biogenesis.

Pathways

RPS6 plays a significant role in the mTOR and PI3K/AKT signaling pathways. These pathways link nutrient availability to protein synthesis influencing cellular growth and metabolism. RPS6 interacts with proteins like S6K1 which is a downstream target in the mTOR pathway to modulate translation and cell growth processes.

Associated diseases and disorders

RPS6 has connections to cancer and metabolic syndromes. Abnormal RPS6 activation can lead to dysregulated cell growth contributing to oncogenesis. In the context of cancer mTOR signaling where RPS6 functions is often upregulated leading to uncontrolled proliferation. Moreover in metabolic disorders the disrupted pathway activity involves other proteins like AKT fostering insulin resistance and metabolic imbalance.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S240 + S244) antibody [SP45] - BSA and Azide free (ab236438), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S240 + S244) antibody [SP45] - BSA and Azide free (ab236438)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling RPS6 with Anti-RPS6 (phospho S240 + S244) antibody [SP45] ab81081 at 1/50 dilution (3.86 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining on human breast carcinoma without lambda phosphatase treatment (image A); no signal can be observed after treatment with lambda phosphatase (image B), performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab81082 for 30 mins at room temperature. This image was generated using Anti-RPS6 (phospho S240 + S244) antibody [SP45] ab81081, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S240 + S244) antibody [SP45] - BSA and Azide free (ab236438), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S240 + S244) antibody [SP45] - BSA and Azide free (ab236438)

    Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for RPS6 (phospho S240 + S244) using Anti-RPS6 (phospho S240 + S244) antibody [SP45] ab81081 at 1/50 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RPS6 (phospho S240 + S244) antibody [SP45] ab81081).

  • Immunocytochemistry/ Immunofluorescence - Anti-RPS6 (phospho S240 + S244) antibody [SP45] - BSA and Azide free (ab236438), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RPS6 (phospho S240 + S244) antibody [SP45] - BSA and Azide free (ab236438)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) treated with lamda phosphatase cells labeling RPS6 with purified Anti-RPS6 (phospho S240 + S244) antibody [SP45] ab81081 at 1/500 (1.3 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RPS6 (phospho S240 + S244) antibody [SP45] ab81081).

  • Flow Cytometry (Intracellular) - Anti-RPS6 (phospho S240 + S244) antibody [SP45] - BSA and Azide free (ab236438), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-RPS6 (phospho S240 + S244) antibody [SP45] - BSA and Azide free (ab236438)

    Intracellular Flow Cytometry analysis of MCF7 grown in serum-free media overnight, then grown in 20% FBS media for 30 minutes labeling RPS6 with purified Anti-RPS6 (phospho S240 + S244) antibody [SP45] ab81081 at 1/640 dilution (1.00μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black. Unlableled control -Unlabelled cells / blue. Untreated cells (Green). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RPS6 (phospho S240 + S244) antibody [SP45] ab81081).

  • Dot Blot - Anti-RPS6 (phospho S240 + S244) antibody [SP45] - BSA and Azide free (ab236438), expandable thumbnail

    Dot Blot - Anti-RPS6 (phospho S240 + S244) antibody [SP45] - BSA and Azide free (ab236438)

    Dot blot analysis of RPS6 (phospho S240 + S244) peptide (Lane 1), RPS6 (phospho S240) peptide (Lane 2), RPS6 (phospho S244) peptide (Lane 3) and RPS6 non-phospho peptide (Lane 4) RPS6 labeling (phospho S240 + S244) with purified Anti-RPS6 (phospho S240 + S244) antibody [SP45] ab81081 at 0.643 μg/mL. Goat Anti-Rabbit IgG H&L (HRP) ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at 0.01 μg/mL.

    Blocking and dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RPS6 (phospho S240 + S244) antibody [SP45] ab81081).

  • ELISA - Anti-RPS6 (phospho S240 + S244) antibody [SP45] - BSA and Azide free (ab236438), expandable thumbnail

    ELISA - Anti-RPS6 (phospho S240 + S244) antibody [SP45] - BSA and Azide free (ab236438)

    ELISA antigen dose-response curve using purified Anti-RPS6 (phospho S240 + S244) antibody [SP45] ab81081 at 0.1-1000 ng/mL. Antigen concentration of 0-10000 ng/mL. A Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) (Jackson ImmunoResearch) (1/2500) was used as the secondary antibody.

    RPS (phospho S240 + S244): RA-S(p)-TSK-S(p)-ESSQKC
    RPS (phospho S240) : RA-S(p)-TSKSESSQKC
    RPS (phospho S244): RASTSK-S(p)-ESSQKC
    RPS non-phospho: RASTSKSESSQKC

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RPS6 (phospho S240 + S244) antibody [SP45] ab81081).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com