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AB251342

Anti-RPS8 antibody [EPR17334] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal RPS8 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

OK/SW-cl.83, RPS8, Small ribosomal subunit protein eS8, 40S ribosomal protein S8

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-RPS8 antibody [EPR17334] - BSA and Azide free (AB251342)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPS8 antibody [EPR17334] - BSA and Azide free (AB251342)

This data was developed using ab201454, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton-X100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling RPS8 with ab201454 at 1/600 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/500 dilution (green). Confocal image showing cytoplasmic staining on Jurkat cell line. The nuclear counter stain is DAPI (blue). Tubulin is stained with ab7291 anti-Tubulin (mouse mAb) at 1/1000 dilution, followed by AlexaFluor®594 Goat anti-Mouse secondary antibody (ab150120) at 1/500 dilution (red).
-ve control 1 : ab201454 at 1/600 dilution followed by AlexaFluor®594 Goat anti-Mouse secondary antibody (ab150120) at 1/500 dilution.
-ve control 2 : ab7291 anti-Tubulin (mouse mAb) at 1/1000 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/500 dilution.

Flow Cytometry (Intracellular) - Anti-RPS8 antibody [EPR17334] - BSA and Azide free (AB251342)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RPS8 antibody [EPR17334] - BSA and Azide free (AB251342)

This data was developed using ab201454, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed A549 (Human lung carcinoma) cells labeling RPS8 with ab201454 at 1/250 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-RPS8 antibody [EPR17334] - BSA and Azide free (AB251342)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPS8 antibody [EPR17334] - BSA and Azide free (AB251342)

This data was developed using ab201454, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton-X100 permeabilized A549 (Human lung carcinoma) cells labeling RPS8 with ab201454 at 1/600 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/500 dilution (green). Confocal image showing cytoplasmic staining on A549 cell line. The nuclear counter stain is DAPI (blue). Tubulin is stained with ab7291 anti-Tubulin (mouse mAb) at 1/1000 dilution, followed by AlexaFluor®594 Goat anti-Mouse secondary antibody (ab150120) at 1/500 dilution (red).
-ve control 1 : ab201454 at 1/600 dilution followed by AlexaFluor®594 Goat anti-Mouse secondary antibody (ab150120) at 1/500 dilution.
-ve control 2 : ab7291 anti-Tubulin (mouse mAb) at 1/1000 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/500 dilution.

Immunoprecipitation - Anti-RPS8 antibody [EPR17334] - BSA and Azide free (AB251342)
  • IP

Supplier Data

Immunoprecipitation - Anti-RPS8 antibody [EPR17334] - BSA and Azide free (AB251342)

This data was developed using ab201454, the same antibody clone in a different buffer formulation.

RPS8 was immunoprecipitated from 1mg of HEK293 (Human embryonic kidney) whole cell lysate with ab201454 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab201454 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1500 dilution.

Lane 1 : HEK293 whole cell lysate 10 μg (Input). Lane 2 : ab201454 IP in HEK293 whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab201454 in HEK293 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-RPS8 antibody [EPR17334] (<a href='/en-us/products/primary-antibodies/rps8-antibody-epr17334-ab201454'>ab201454</a>)

Predicted band size: 24 kDa

false

Western blot - Anti-RPS8 antibody [EPR17334] - BSA and Azide free (AB251342)
  • WB

Supplier Data

Western blot - Anti-RPS8 antibody [EPR17334] - BSA and Azide free (AB251342)

This data was developed using ab201454, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RPS8 antibody [EPR17334] (<a href='/en-us/products/primary-antibodies/rps8-antibody-epr17334-ab201454'>ab201454</a>) at 1/10000 dilution

Lane 1:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

Lane 2:

HEK293 (Human embryonic kidney) whole cell lysate at 20 µg

Lane 3:

A549 (Human lung carcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 24 kDa

Observed band size: 24 kDa

false

Exposure time: 10s

Western blot - Anti-RPS8 antibody [EPR17334] - BSA and Azide free (AB251342)
  • WB

Supplier Data

Western blot - Anti-RPS8 antibody [EPR17334] - BSA and Azide free (AB251342)

This data was developed using ab201454, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RPS8 antibody [EPR17334] (<a href='/en-us/products/primary-antibodies/rps8-antibody-epr17334-ab201454'>ab201454</a>) at 1/10000 dilution

Lane 1:

Human cerebellum lysate at 10 µg

Lane 2:

Human pancreas lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 24 kDa

Observed band size: 24 kDa

false

Exposure time: 10s

Western blot - Anti-RPS8 antibody [EPR17334] - BSA and Azide free (AB251342)
  • WB

Supplier Data

Western blot - Anti-RPS8 antibody [EPR17334] - BSA and Azide free (AB251342)

This data was developed using ab201454, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RPS8 antibody [EPR17334] (<a href='/en-us/products/primary-antibodies/rps8-antibody-epr17334-ab201454'>ab201454</a>) at 1/2000 dilution

Lane 1:

C6 (Rat glial tumor cells) whole cell lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 24 kDa

Observed band size: 24 kDa

false

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17334

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IP, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab251342 is the carrier-free version of ab201454.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ribosomal protein S8 (RPS8) also known as S8 is a component of the small 40S subunit of the eukaryotic ribosome. This protein has a mass of approximately 20 kDa and is widely expressed in most tissues reflecting its fundamental role in cellular processes. RPS8 belongs to the ribosomal protein S8P family and plays a mechanical role in the assembly and function of the ribosome where it contributes to protein synthesis by facilitating mRNA translation.
Biological function summary

Ribosomal protein S8 is fundamental in translation an essential cellular process that translates mRNA sequences into polypeptide chains. As part of the ribosomal 40S subunit RPS8 aids in the initiation of translation by interacting with other ribosomal proteins and rRNA to form a functional ribosome. This subunit along with the 60S large subunit comprises the active ribosome complex engaged in translating genetic information into the proteome.

Pathways

RPS8 is involved in the protein synthesis pathway a critical driver of cellular proliferation and metabolism. It participates actively in the mRNA surveillance pathway ensuring the efficiency and accuracy of translation. It interacts with other proteins such as RPS6 and RPS14 which are also constituents of the small ribosomal subunit acting synergistically to regulate the efficacy of the translation process. Moreover RPS8 involvement in the ribosome biogenesis pathway emphasizes its integral role in cellular growth and division.

Alterations in ribosomal protein S8 are connected to ribosomopathies a group of disorders characterized by defective ribosome function. Dysregulation or mutations in RPS8 can lead to Diamond-Blackfan anemia a rare congenital disorder causing impaired erythropoiesis. Additionally RPS8 has correlations with cancer where aberrant expression levels contribute to tumorigenesis. In cancer RPS8 might interact with oncogenic proteins such as MYC heightening the proliferative potential of cancer cells.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the small ribosomal subunit (PubMed : 23636399). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).
See full target information RPS8
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 22:800 PubMed39731111

2024

Role of PCBP2 in regulating nanovesicles loaded with curcumin to mitigate neuroferroptosis in neural damage caused by heat stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Guo,Yizhan Wu,Guangjun Wang,Jiangwei Liu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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