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Rabbit Recombinant Monoclonal RPS8 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-RPS8 antibody [EPR17334] - BSA and Azide free (AB251342), expandable thumbnail
  • Western blot - Anti-RPS8 antibody [EPR17334] - BSA and Azide free (AB251342), expandable thumbnail
  • Western blot - Anti-RPS8 antibody [EPR17334] - BSA and Azide free (AB251342), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-RPS8 antibody [EPR17334] - BSA and Azide free (AB251342), expandable thumbnail
  • Immunoprecipitation - Anti-RPS8 antibody [EPR17334] - BSA and Azide free (AB251342), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Mouse
Expected
Expected
Tested
Expected
Rat
Expected
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Component of the small ribosomal subunit (PubMed:23636399). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:23636399). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797).

Alternative names

OK/SW-cl.83, RPS8, Small ribosomal subunit protein eS8, 40S ribosomal protein S8

Recommended products

Rabbit Recombinant Monoclonal RPS8 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR17334
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab251342 is the carrier-free version of Anti-RPS8 antibody [EPR17334] ab201454.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Ribosomal protein S8 (RPS8) also known as S8 is a component of the small 40S subunit of the eukaryotic ribosome. This protein has a mass of approximately 20 kDa and is widely expressed in most tissues reflecting its fundamental role in cellular processes. RPS8 belongs to the ribosomal protein S8P family and plays a mechanical role in the assembly and function of the ribosome where it contributes to protein synthesis by facilitating mRNA translation.

Biological function summary

Ribosomal protein S8 is fundamental in translation an essential cellular process that translates mRNA sequences into polypeptide chains. As part of the ribosomal 40S subunit RPS8 aids in the initiation of translation by interacting with other ribosomal proteins and rRNA to form a functional ribosome. This subunit along with the 60S large subunit comprises the active ribosome complex engaged in translating genetic information into the proteome.

Pathways

RPS8 is involved in the protein synthesis pathway a critical driver of cellular proliferation and metabolism. It participates actively in the mRNA surveillance pathway ensuring the efficiency and accuracy of translation. It interacts with other proteins such as RPS6 and RPS14 which are also constituents of the small ribosomal subunit acting synergistically to regulate the efficacy of the translation process. Moreover RPS8 involvement in the ribosome biogenesis pathway emphasizes its integral role in cellular growth and division.

Associated diseases and disorders

Alterations in ribosomal protein S8 are connected to ribosomopathies a group of disorders characterized by defective ribosome function. Dysregulation or mutations in RPS8 can lead to Diamond-Blackfan anemia a rare congenital disorder causing impaired erythropoiesis. Additionally RPS8 has correlations with cancer where aberrant expression levels contribute to tumorigenesis. In cancer RPS8 might interact with oncogenic proteins such as MYC heightening the proliferative potential of cancer cells.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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