JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB74711

Anti-RPS9 antibody

Be the first to review this product! Submit a review

|

(5 Publications)

Rabbit Polyclonal RPS9 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human 40S ribosomal protein S9.

View Alternative Names

Small ribosomal subunit protein uS4, 40S ribosomal protein S9, RPS9

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-RPS9 antibody (AB74711)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RPS9 antibody (AB74711)

Immunofluorescence analysis of A549 cells, using ab74711 at 1/500-1/1000 dilution in the presence (+) or absence (-) of immunising peptide.

Western blot - Anti-RPS9 antibody (AB74711)
  • WB

Unknown

Western blot - Anti-RPS9 antibody (AB74711)

All lanes:

Western blot - Anti-RPS9 antibody (ab74711) at 1/500 dilution

Lane 1:

extracts from HepG2 cells at 20 µg

Lane 2:

extracts from COLO cells at 20 µg

Lane 3:

extracts from HT-29 cells at 20 µg

Lane 4:

extracts from HeLa cells at 30 µg

Lane 5:

extracts from HUVEC cells at 20 µg

Lane 6:

extracts from HepG2 cells at 20 µg with immunizing peptide

Predicted band size: 23 kDa

Observed band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human 40S ribosomal protein S9. The exact immunogen used to generate this antibody is proprietary information.

P46781

style
left-column

Alternative Products

Primary Antibodies

AB157125

Anti-RPS9 antibody

Western blot - Anti-RPS9 antibody (AB157125)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB210745

Anti-RPS5 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RPS5 antibody (AB210745)

  • 0
  • 0
Primary Antibodies

AB241412

Anti-RPL3 antibody

Immunoprecipitation - Anti-RPL3 antibody (AB241412)

  • 0
  • 0
Proteins & Peptides

AB137146

Recombinant Human RPS5 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human RPS5 protein (His tag N-Terminus) (AB137146)

  • 0
  • 0
Primary Antibodies

AB80592

Anti-Ku80 antibody [EPR3468]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Ku80 antibody [EPR3468] (AB80592)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPS9 also known as Ribosomal Protein S9 plays an important role in protein synthesis by being part of the small 40S ribosomal subunit. It has a molecular mass of approximately 22 kDa. This protein is expressed in various tissues throughout the body and is essential for ribosome function. Its primary mechanical function involves the formation of ribosomes which are key components in the translation process translating the genetic code into proteins.
Biological function summary

As a component of the ribosomal assembly RPS9 is essential for maintaining the structure and function of ribosomes. It forms a part of the ribonucleoprotein complex that ensures the fidelity and accuracy of mRNA translation during protein synthesis. This activity is vital for cell growth and proliferation as it directly influences protein production efficiency. Any disruptions in this process can lead to misfolded proteins or incomplete translations affecting cell functionality.

Pathways

The function of RPS9 is critical in the mRNA processing and translation pathways. It associates closely with other ribosomal proteins such as RPS3 and RPL11 participating in the synthesis and processing of proteins required for various cellular activities. This involvement impacts key pathways like the mTOR signaling pathway which controls cell growth and metabolism and the p53 signaling pathway which is involved in cell cycle regulation and stress responses.

The role of RPS9 implicates it in cancer and Diamond-Blackfan anemia. Mutations or dysregulation of RPS9 can lead to abnormal cell proliferation contributing to oncogenesis. RPS9 has connections with the p53 protein pathway which plays a significant role in tumorigenesis when mutated or impaired. In Diamond-Blackfan anemia defects in ribosomal protein function including that of RPS9 result in bone marrow failure and defective erythropoiesis affecting red blood cell production.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Component of the small ribosomal subunit (PubMed : 23636399). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).
See full target information 40S ribosomal protein S9
style
left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 5:564 PubMed35681048

2022

Stabilization of CDK6 by ribosomal protein uS7, a target protein of the natural product fucoxanthinol.

Applications

Unspecified application

Species

Unspecified reactive species

Yosuke Iizumi,Yoshihiro Sowa,Wakana Goi,Yuichi Aono,Motoki Watanabe,Yoichi Kurumida,Tomoshi Kameda,Kenichi Akaji,Masatoshi Kitagawa,Toshiyuki Sakai

Disease markers 2020:8259820 PubMed32714467

2020

In Vitro and In Vivo Efficacy of DNA Damage Repair Inhibitor Veliparib in Combination with Artesunate against .

Applications

Unspecified application

Species

Unspecified reactive species

Y F Li,L M Wen,J Zhao,G D Lv,S Lu,S Lu,X Zheng,B Chen,C Y Tian,Y H Gong,H J Gao,J H Wang

PloS one 8:e73219 PubMed24009741

2013

The flavonoid apigenin downregulates CDK1 by directly targeting ribosomal protein S9.

Applications

WB, Flow Cyt

Species

Unspecified reactive species, Unspecified reactive species

Yosuke Iizumi,Masakatsu Oishi,Tomoyuki Taniguchi,Wakana Goi,Yoshihiro Sowa,Toshiyuki Sakai

In vitro cellular & developmental biology. Animal 47:132-8 PubMed21082278

2010

Tagging of functional ribosomes in living cells by HaloTag® technology.

Applications

WB

Species

Human

Simone Gallo,Anne Beugnet,Stefano Biffo

Journal of the American Chemical Society 132:656-66 PubMed20028024

2009

Activity-based proteome profiling of potential cellular targets of Orlistat--an FDA-approved drug with anti-tumor activities.

Applications

Unspecified application

Species

Unspecified reactive species

Peng-Yu Yang,Kai Liu,Mun Hong Ngai,Martin J Lear,Markus R Wenk,Shao Q Yao
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com