Rabbit Polyclonal MSS1 antibody. Suitable for WB and reacts with Purified native protein - Yeast, Schizosaccharomyces pombe, Saccharomyces cerevisiae samples. Immunogen corresponding to Recombinant Fragment Protein within Saccharomyces cerevisiae S288C RPT1 aa 1-100.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Standard buffer
- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Saccharomyces cerevisiae S288C RPT1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P33299
Reactivity data
Select an application| WB | |
|---|---|
| Purified native protein - Yeast | Tested |
| Saccharomyces cerevisiae | Expected |
| Schizosaccharomyces pombe | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Purified native protein - Yeast | Dilution info 1/10000.00000 - 1/50000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Schizosaccharomyces pombe | Dilution info 1/10000.00000 - 1/50000.00000 | Notes - |
Species Saccharomyces cerevisiae | Dilution info 1/10000.00000 - 1/50000.00000 | Notes - |
Target data
Function
The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity).
Targets
Rpt1
Alternative names
26S proteasome regulatory subunit 7 homolog, Protein CIM5, Tat-binding homolog 3, YKL145W, RPT1, CIM5, YTA3
Recommended products
Rabbit Polyclonal MSS1 antibody. Suitable for WB and reacts with Purified native protein - Yeast, Schizosaccharomyces pombe, Saccharomyces cerevisiae samples. Immunogen corresponding to Recombinant Fragment Protein within Saccharomyces cerevisiae S288C RPT1 aa 1-100.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Standard buffer
- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Saccharomyces cerevisiae S288C RPT1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P33299
- Purity
Whole antiserum
- Specificity
At optimal dilution there is no cross-reactivity with other members of the YTA family.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Dilute to working strength with phosphate buffered saline pH 7.2-7.4 and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used).
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Rpt1 also known as PSMC2 or 19S proteasome regulatory subunit 2 is a protein involved in the ATPase activity within the 19S regulatory particle of the proteasome. This protein has a molecular weight of approximately 46 kDa and plays an important role in protein degradation. Rpt1 is expressed ubiquitously in various tissues reflecting its importance in cellular functions. The protein's structure includes an ATPase domain which is necessary for energy-dependent unfolding of substrates during proteolysis.
- Biological function summary
This protein plays an important role in the function of the ubiquitin-proteasome system (UPS) a complex mechanism responsible for targeted protein degradation. Rpt1 is a component of the 26S proteasome specifically part of the 19S regulatory complex and it assists in the recognition and unfolding of ubiquitinated proteins before they are translocated to the 20S core particle for degradation. The protein's role in UPS is essential for maintaining cellular protein homeostasis by controlling protein levels and quality.
- Pathways
The protein is integral to the ubiquitin-proteasome pathway which is essential for various cellular processes like cell cycle control signal transduction and stress response. Within this pathway Rpt1 works closely with other proteasome subunits and associated proteins such as ubiquitin and Rpn1. The protein's activity ensures the timely degradation of regulatory proteins and misfolded proteins allowing for regulation of protein quality and turnover within the cell.
- Associated diseases and disorders
Rpt1 has been implicated in neurodegenerative diseases such as Alzheimer's and certain types of cancers. The dysregulation of proteasome function involving Rpt1 may lead to the accumulation of protein aggregates associated with neuronal degeneration. In cancer alterations in proteasome activity mediated by Rpt1 and related proteins like Rpt2 can affect cell cycle progression and apoptosis evasion contributing to tumorigenesis. Understanding these connections provides insight into potential therapeutic targets within these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-Rpt1 antibody (ab22678)
All lanes: Western blot - Anti-Rpt1 antibody (ab22678) at 1/25000 dilution
All lanes: Purified yeast 26S proteasome preparation
Developed using the ECL technique.
Predicted band size: 52 kDa
Exposure time: 1min
Downloads
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