Rabbit Polyclonal TBP-1 antibody. Suitable for WB and reacts with Fungi, Arabidopsis thaliana, Saccharomyces cerevisiae samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Saccharomyces cerevisiae S288C RPT5 aa 1-100.
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Publications
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- Nature plants 9:2059-20702023Evolution of a plant growth-regulatory protein interaction specificity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhe Ji,Eric J Belfield,Siyu Zhang,Jacques Bouvier,Shan Li,Jason Schnell,Xiangdong Fu,Nicholas P HarberdPubMed 37903985
- The Plant cell 31:2411-24292019Arabidopsis ALIX Regulates Stomatal Aperture and Turnover of Abscisic Acid Receptors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarta García-León,Laura Cuyas,Diaa Abd El-Moneim,Lesia Rodriguez,Borja Belda-Palazón,Eva Sanchez-Quant,Yolanda Fernández,Brice Roux,Ángel María Zamarreño,José María García-Mina,Laurent Nussaume,Pedro L Rodriguez,Javier Paz-Ares,Nathalie Leonhardt,Vicente RubioPubMed 31363038
- Frontiers in plant science 8:21142018Constitutive Expression of miR408 Improves Biomass and Seed Yield in Arabidopsis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhaoqing Song,Lifen Zhang,Yulong Wang,Haixia Li,Shuang Li,Huijie Zhao,Huiyong ZhangPubMed 29422907
- Molecular plant 9:1395-14052016Repression of MYBL2 by Both microRNA858a and HY5 Leads to the Activation of Anthocyanin Biosynthetic Pathway in Arabidopsis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYulong Wang et. al.PubMed 27450422
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: Whole serum
- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Saccharomyces cerevisiae S288C RPT5 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P33297
Reactivity data
Select an application| WB | |
|---|---|
| Arabidopsis thaliana | Expected |
| Fungi | Expected |
| Saccharomyces cerevisiae | Expected |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Fungi | Dilution info 1/10000.00000 - 1/50000.00000 | Notes - |
Species Arabidopsis thaliana | Dilution info 1/10000.00000 - 1/50000.00000 | Notes - |
Species Saccharomyces cerevisiae | Dilution info 1/10000.00000 - 1/50000.00000 | Notes - |
Target data
Function
The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity).
Alternative names
26S proteasome regulatory subunit 6A, Tat-binding protein homolog 1, TBP-1, YOR3258W, O3258, YOR117W, YTA1, RPT5
Recommended products
Rabbit Polyclonal TBP-1 antibody. Suitable for WB and reacts with Fungi, Arabidopsis thaliana, Saccharomyces cerevisiae samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Saccharomyces cerevisiae S288C RPT5 aa 1-100.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: Whole serum
- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Saccharomyces cerevisiae S288C RPT5 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P33297
- Purity
Whole antiserum
- Specificity
ab22676 reacts with 19S regulator ATPase subunit Rpt5/TBP-1 (Yta1).
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Rpt5 also known as TBP-1 (Tat-binding protein 1) is part of the 19S regulatory particle of the proteasome complex with a molecular mass of about 50 kDa. This ATPase plays a role in protein degradation by facilitating the unfolding and translocation of ubiquitinated proteins into the 20S proteasome core. Rpt5/TBP-1 is ubiquitously expressed across different tissues emphasizing its importance in maintaining protein homeostasis throughout the body.
- Biological function summary
Rpt5/TBP-1 contributes to protein quality control by being a component of the 26S proteasome complex. This complex is responsible for recognizing and degrading proteins tagged with ubiquitin regulating diverse cellular processes like cell cycle progression signal transduction and stress responses. Within the proteasome complex Rpt5/TBP-1 helps in substrate recognition and unfolding which are necessary steps for the accurate degradation of target proteins.
- Pathways
Rpt5/TBP-1 is intricately involved in the ubiquitin-proteasome pathway a critical mechanism for protein turnover in cells. This pathway interacts with other key signaling pathways such as the NF-kB signaling that regulates immune response and inflammation. Rpt5/TBP-1 works alongside other regulatory particle components such as Rpt1 and Rpt2 facilitating the energy-dependent steps required for substrate processing in the proteasome.
- Associated diseases and disorders
Rpt5/TBP-1 is connected to neurodegenerative diseases like Alzheimer's and Parkinson's where protein misfolding and aggregation occur. Disrupted proteasome activity due to Rpt5/TBP-1 dysfunction may contribute to these conditions. Additionally Rpt5/TBP-1 has connections to cancer where altered proteasome function can affect cell proliferation and apoptosis. In cancer proteins such as p53 which regulates cell cycle machinery can interact with proteasome-related dysfunction impacting cancer progression and treatment outcomes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-Rpt5/TBP-1 antibody (ab22676)
All lanes: Western blot - Anti-Rpt5/TBP-1 antibody (ab22676) at 1/25000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa, 48 kDa
Observed band size: 48 kDa
Exposure time: 1min
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