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AB181121

Anti-RPUSD3 antibody [EPR12911]

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(1 Publication)

Rabbit Recombinant Monoclonal RPUSD3 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Mitochondrial mRNA pseudouridine synthase RPUSD3, RNA pseudouridylate synthase domain-containing protein 3, RPUSD3

3 Images
Flow Cytometry (Intracellular) - Anti-RPUSD3 antibody [EPR12911] (AB181121)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RPUSD3 antibody [EPR12911] (AB181121)

Intracellular Flow Cytometry analysis of U87-MGcells using ab181121at a 1/290 dilution (red) or aRabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary usedat a 1/150 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-RPUSD3 antibody [EPR12911] (AB181121)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPUSD3 antibody [EPR12911] (AB181121)

Immunofluorescence analysis of SH-SY5Y cells (fixative 4% paraformaldehyde) labeling RPUSD3 with ab181121 at a 1/250 dilution. Goat anti rabbit IgG (Alexa Fluor® 555) secondary used at a 1/200 diution.

Western blot - Anti-RPUSD3 antibody [EPR12911] (AB181121)
  • WB

Supplier Data

Western blot - Anti-RPUSD3 antibody [EPR12911] (AB181121)

All lanes:

Western blot - Anti-RPUSD3 antibody [EPR12911] (ab181121) at 1/1000 dilution

Lane 1:

U87-MG lysate at 20 µg

Lane 2:

SH-SY5Y lysate at 20 µg

Lane 3:

293T lysate at 20 µg

Lane 4:

Human fetal brain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 38 kDa

false

  • Carrier free

    Anti-RPUSD3 antibody [EPR12911] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12911

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/290", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPUSD3 also known as RNA pseudouridylate synthase domain containing 3 is an enzyme involved in RNA modification. This protein has a mass of approximately 60 kDa and exhibits expression in various tissues including the brain heart and skeletal muscle. The enzyme plays a role in catalyzing the isomerization of uridine to pseudouridine in RNA molecules an important step for RNA stability and function.
Biological function summary

RPUSD3 contributes to the modification of ribosomal RNA (rRNA) enhancing its structural stability and translation fidelity. This protein operates as part of a ribonucleoprotein complex engaging with other molecules to facilitate the precise modification processes necessary for proper RNA function. These modifications are important for cellular health and protein biosynthesis.

Pathways

The enzymatic activity of RPUSD3 integrates into the RNA modification pathway influencing an important process for gene expression regulation. It works alongside proteins like dyskerin which also participates in pseudouridine synthesis within the small subunit processome. This involvement positions RPUSD3 as a significant player in rRNA processing ensuring the correct assembly of ribosomes.

Alterations in RPUSD3 activity have been connected to certain neurodevelopmental disorders. Research suggests that abnormal RPUSD3 function may contribute to intellectual disability where it is seen to interact with proteins involved in neuronal development such as FMRP. Additionally this protein's altered activity associates with metabolic disorders which highlights its relevance to maintaining normal metabolic processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes uridine to pseudouridine isomerization (pseudouridylation) of specific mitochondrial mRNAs (mt-mRNAs), a post-transcriptional modification necessary for their translation. Acts at position 390 in COXI mt-mRNA and at position 697-699 in mitochondrial COXIII mt-mRNA (PubMed : 27974379). As a component of a functional protein-RNA module, consisting of RCC1L, NGRN, RPUSD3, RPUSD4, TRUB2, FASTKD2 and 16S mitochondrial ribosomal RNA (16S mt-rRNA), controls 16S mt-rRNA abundance and may play a role in mitochondrial ribosome biogenesis (PubMed : 27667664).
See full target information RPUSD3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Renal physiology 318:F457-F467 PubMed31760768

2019

Nitro-oleic acid inhibits the high glucose-induced epithelial-mesenchymal transition in peritoneal mesothelial cells and attenuates peritoneal fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyan Su,Haiping Wang,ZiYan Feng,Jing Sun
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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