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AB205977

Anti-RRAD antibody [EPR12856] - BSA and Azide free

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Rabbit Recombinant Monoclonal RRAD antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

RAD, RRAD, GTP-binding protein RAD, RAD1, Ras associated with diabetes

3 Images
Western blot - Anti-RRAD antibody [EPR12856] - BSA and Azide free (AB205977)
  • WB

Lab

Western blot - Anti-RRAD antibody [EPR12856] - BSA and Azide free (AB205977)

This data was developed using ab177151, the same antibody clone in a different buffer formulation.

We are unsure how to define the extra bands.

All lanes:

Western blot - Anti-RRAD antibody [EPR12856] (<a href='/en-us/products/primary-antibodies/rrad-antibody-epr12856-ab177151'>ab177151</a>) at 1/5000 dilution

All lanes:

A-673 (Human muscle Ewing's Sarcoma) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/2000 dilution

Predicted band size: 33 kDa

Observed band size: 33 kDa

false

Western blot - Anti-RRAD antibody [EPR12856] - BSA and Azide free (AB205977)
  • WB

Supplier Data

Western blot - Anti-RRAD antibody [EPR12856] - BSA and Azide free (AB205977)

This data was developed using ab177151, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RRAD antibody [EPR12856] (<a href='/en-us/products/primary-antibodies/rrad-antibody-epr12856-ab177151'>ab177151</a>) at 1/1000 dilution

Lane 1:

Human fetal heart lysate at 10 µg

Lane 2:

Human fetal lung lysate at 10 µg

Lane 3:

A673 lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 33 kDa

false

Western blot - Anti-RRAD antibody [EPR12856] - BSA and Azide free (AB205977)
  • WB

Lab

Western blot - Anti-RRAD antibody [EPR12856] - BSA and Azide free (AB205977)

This data was developed using ab177151, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RRAD antibody [EPR12856] (<a href='/en-us/products/primary-antibodies/rrad-antibody-epr12856-ab177151'>ab177151</a>) at 1/5000 dilution

All lanes:

Human fetal lung tissue lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 33 kDa

Observed band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12856

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab205977 is the carrier-free version of ab177151.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RRAD also known as Ras-related associated with diabetes is a small GTPase belonging to the Ras superfamily. It weighs approximately 29 kDa and exhibits expression in cardiac and skeletal muscle tissues as well as the nervous system. This protein functions mechanically by binding and hydrolyzing GTP a process critical for its role in intracellular signaling. RRAD is known for its ability to affect various molecular interactions through its GTPase activity.
Biological function summary

RRAD regulates glucose transport and metabolism influencing energy balance in cells. It does not integrate into a larger protein complex but instead acts on its own to modify glucose transporter activity. RRAD impacts how cells take up and use glucose which is significant for maintaining cellular energy requirements and contributes to processes that control cell growth and survival.

Pathways

Understanding the signaling mechanisms of RRAD involves examining the insulin signaling pathway and the glucose metabolism pathway. RRAD impacts insulin receptor signaling and modulation of glucose transporter type 4 (GLUT4) activity. RRAD holds a relationship with other proteins such as AKT and PI3K in these pathways which play critical roles in transmitting signals that manage glucose uptake and regulation.

The alteration in RRAD expression or function associates with diabetes and cardiovascular diseases. In diabetes abnormal RRAD function affects insulin signaling contributing to insulin resistance. In cardiovascular disease RRAD dysregulation can influence cardiac function through its impact on energy metabolism. The protein interacts with others like GLUT4 and AKT which are relevant in these disease contexts implicating RRAD in pathways that influence disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May regulate basal voltage-dependent L-type Ca(2+) currents and be required for beta-adrenergic augmentation of Ca(2+) influx in cardiomyocytes, thereby regulating increases in heart rate and contractile force (By similarity). May play an important role in cardiac antiarrhythmia via the strong suppression of voltage-gated L-type Ca(2+) currents (By similarity). Regulates voltage-dependent L-type calcium channel subunit alpha-1C trafficking to the cell membrane (By similarity). Inhibits cardiac hypertrophy through the calmodulin-dependent kinase II (CaMKII) pathway (PubMed : 18056528). Inhibits phosphorylation and activation of CAMK2D (PubMed : 18056528).
See full target information RRAD
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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