Anti-RRAS antibody

• IP

Supplier Data

Immunoprecipitation - Anti-RRAS antibody (AB191791)

RRAS was immunoprecipitated from Human fetal kidney lysate with ab191791 at 1/60 dilution. Western blot was performed from the immunoprecipitate using ab191399 at 1/500 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : Human fetal kidney lysate 10 μg (Input). Lane 2 : ab191791 IP in Human fetal kidney lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab191791 in Human fetal kidney lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.

ab191791 was used to Immunoprecipitate RRAS. These precipitates were resolved with ab191399.

All lanes:

Immunoprecipitation - Anti-RRAS antibody (ab191791)

Predicted band size: 23 kDa

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• IP

Supplier Data

Immunoprecipitation - Anti-RRAS antibody (AB191791)

RRAS was immunoprecipitated from Human fetal kidney lysate with ab191791 at 1/60 dilution. Western blot was performed from the immunoprecipitate using ab191791 at 1/500 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : Human fetal kidney lysate 10 μg (Input). Lane 2 : ab191791 IP in Human fetal kidney lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab191791 in Human fetal kidney lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-RRAS antibody (ab191791)

Predicted band size: 23 kDa

false

• WB

Supplier Data

Western blot - Anti-RRAS antibody (AB191791)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RRAS antibody (ab191791) at 1/500 dilution

Lane 1:

Human fetal kidney lysate at 10 µg

Lane 2:

Rat heart lysate at 10 µg

Lane 3:

Mouse spleen lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 23 kDa

Observed band size: 23 kDa

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Exposure time: 1min

• WB

Lab

Western blot - Anti-RRAS antibody (AB191791)

Western blot : Anti-RRAS antibody ab191791 staining at 1/500 dilution, shown in green; Mouse anti alpha Tubulin (ab7291) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 26 kDa in Wild-type A549 UNBOILED cell lysates with no signal observed at this size in RRAS knockout A549 UNBOILED cell line.

To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-RRAS antibody (ab191791) at 1/500 dilution

Lane 1:

Wild-type A549 UNBOILED at 20 µg

Lane 2:

RRAS knockout A549 UNBOILED at 20 µg

Lane 3:

U-87 MG at 20 µg

Lane 4:

HeLa at 20 µg

Lane 5:

Raji at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 23 kDa

Observed band size: 26 kDa

false