AB224354

Anti-RRBP1 antibody

Name: Anti-RRBP1 antibody (ab224354) | Abcam
Brand: Abcam Limited
SKU: AB224354

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(2 Publications)

Rabbit Polyclonal RRBP1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RRBP1 aa 900 to C-terminus.

View Alternative Names

KIAA1398, RRBP1, Ribosome-binding protein 1, 180 kDa ribosome receptor homolog, ES/130-related protein, Ribosome receptor protein, RRp

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRBP1 antibody (AB224354)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRBP1 antibody (AB224354)

Paraffin-embedded human placenta tissue stained for RRBP1 using ab224354 at 1/2500 in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-RRBP1 antibody (AB224354)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RRBP1 antibody (AB224354)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for RRBP1 (greeen) using ab224354 at 4 μg/ml in ICC/IF.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRBP1 antibody (AB224354)

Paraffin-embedded human stomach tissue stained for RRBP1 using ab224354 at 1/2500 in immunohistochemical analysis.

Unknown

Western blot - Anti-RRBP1 antibody (AB224354)

All lanes:

Western blot - Anti-RRBP1 antibody (ab224354) at 1/1000 dilution

All lanes:

Caco-2 (human colorectal adenocarcinoma cell line) cell lysate

Predicted band size: 152 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RRBP1 aa 900 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9P2E9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2500 - 1/5000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RRBP1 also known as ribosome binding protein 1 is a significant endoplasmic reticulum (ER) membrane protein involved in ribosome binding and translocation of newly synthesized proteins. This protein also goes by the name p180 and has a molecular mass of approximately 180 kDa. RRBP1 is predominantly expressed in cells with high secretory activity such as those in the pancreas and liver where it assists in the robust production and processing of proteins required for secretion.

Biological function summary

RRBP1 facilitates the initial binding of ribosomes to the ER membrane a process essential for the proper targeting and translocation of proteins entering the secretory pathway. This protein is integral to the ribosome-ER association acting as a scaffold that stabilizes ribosome attachment during translation. RRBP1 functions as part of a larger translocon complex that includes proteins such as Sec61 which together form a channel for translocating polypeptides into the ER lumen or integrating them into the ER membrane.

Pathways

The protein is essential in protein synthesis and secretion pathways. RRBP1 coordinates with signal recognition particle-dependent pathways where it partners with proteins like Sec61 to ensure accurate translocation of secretory and membrane proteins across the ER membrane. In the unfolded protein response (UPR) pathway RRBP1 might also interact with ER chaperones to assist in protein folding and maintain cellular homeostasis under stress conditions.

Abnormalities in RRBP1 expression and function have associations with certain types of cancer including breast cancer where its overexpression contributes to tumor progression. Additionally RRBP1 dysregulation may relate to neurodegenerative diseases through its role in protein folding and ER stress response. Proteins like BiP (Grp78) an important chaperone involved in UPR may link to RRBP1 in these pathological states highlighting potential intersections between ER stress and disease pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane.

See full target information RRBP1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of gastroenterology and hepatology 36:3457-3468 PubMed34340259

2021

Hsa_circ_0016788 regulates hepatocellular carcinoma progression via miR-506-3p/poly-adenosine diphosphate-ribose polymerase.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Chen,Guangsheng Hu,Xin Zhou,Zhong Peng,Wu Wen

Aging 12: PubMed33535184

2021

Evaluation of the prognostic values of solute carrier (SLC) family 39 genes for patients with lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Heng Zhou,Yaoqi Zhu,Huizhong Qi,Liang Liang,Hao Wu,Jingping Yuan,Qingyong Hu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RRBP1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRBP1 antibody (AB224354)

Immunocytochemistry/ Immunofluorescence - Anti-RRBP1 antibody (AB224354)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRBP1 antibody (AB224354)

Western blot - Anti-RRBP1 antibody (AB224354)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

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Cell Lines & Lysates

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise