AB113287

Anti-RREB1 antibody

Name: Anti-RREB1 antibody (ab113287) | Abcam
Brand: Abcam Limited
SKU: AB113287
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal RREB1 antibody. Suitable for IP and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human RREB1 aa 1350-1450.

View Alternative Names

FINB, RREB1, Ras-responsive element-binding protein 1, RREB-1, Finger protein in nuclear bodies, Raf-responsive zinc finger protein LZ321, Zinc finger motif enhancer-binding protein 1, Zep-1

1 Images

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Immunoprecipitation - Anti-RREB1 antibody (AB113287)

Detection of RREB1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab113287 at 6 µg/mg lysate for IP. A Rabbit anti-RREB1 antibody which recognizes a downstream epitope was used at 1 μg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-RREB1 antibody (ab113287)

Predicted band size: 181 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human RREB1 aa 1350-1450. The exact immunogen used to generate this antibody is proprietary information.

Q92766

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RREB1 also known as Ras-responsive element-binding protein 1 is a zinc finger transcription factor with a molecular mass of approximately 197 kDa. RREB1 is highly expressed in various tissues including the pancreas lung and prostate. Its primary role involves binding to specific Ras-responsive elements (RREs) in the DNA regulating the transcription of genes that are pivotal for cell differentiation growth and apoptosis.

Biological function summary

The RREB1 protein interacts with other components to activate or repress target gene expression often acting as part of larger transcriptional complexes. It influences key biological processes such as embryonic development and cellular response to stress by controlling genes involved in these pathways. The interconnected function of RREB1 guides essential cellular decisions including how cells adapt to signaling cues in their environment.

Pathways

RREB1 significantly contributes to the Ras signaling pathway and the MAPK/ERK pathway connecting it to important cellular events like proliferation and differentiation. RREB1 functions alongside proteins such as RAS and MEK collaborating to transmit signals from the cell surface to the nucleus. Through these pathways RREB1 plays a role in adjusting cellular responses to external stimuli influencing gene expression patterns critical for diverse cell functions.

The dysregulation of RREB1 has connections to cancer including prostate and pancreatic cancers. Abnormal RREB1 function can lead to uncontrolled cell proliferation due to its role in gene expression regulation. It has interactions with proteins such as KRAS in these disorders contributing to oncogenic transformations. Additionally alterations in RREB1 are linked to diabetes mellitus where its misregulation might affect insulin signaling pathways impacting glucose metabolism and homeostasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Transcription factor that binds specifically to the RAS-responsive elements (RRE) of gene promoters (PubMed : 10390538, PubMed : 15067362, PubMed : 17550981, PubMed : 8816445, PubMed : 9305772). Represses the angiotensinogen gene (PubMed : 15067362). Negatively regulates the transcriptional activity of AR (PubMed : 17550981). Potentiates the transcriptional activity of NEUROD1 (PubMed : 12482979). Promotes brown adipocyte differentiation (By similarity). May be involved in Ras/Raf-mediated cell differentiation by enhancing calcitonin expression (PubMed : 8816445).

See full target information RREB1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 26:787-797 PubMed34729248

2021

lncRNA MELTF-AS1 facilitates osteosarcoma metastasis by modulating MMP14 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Ding,Taiyuan Liu,Yuan Qu,Zhichen Kang,Lixin Guo,Haina Zhang,Junjie Jiang,Fuling Qu,Wanbao Ge,Shanyong Zhang

Autophagy 18:540-558 PubMed34074205

2021

Silencing PEX26 as an unconventional mode to kill drug-resistant cancer cells and forestall drug resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Michael S Dahabieh,Fan Huang,Christophe Goncalves,Raúl Ernesto Flores González,Sathyen Prabhu,Alicia Bolt,Erminia Di Pietro,Elie Khoury,John Heath,Zi Yi Xu,Joelle Rémy-Sarrazin,Koren K Mann,Alexandre Orthwein,François-Michel Boisvert,Nancy Braverman,Wilson H Miller,Sonia V Del Rincón

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-RREB1 antibody

Immunoprecipitation - Anti-RREB1 antibody (AB113287)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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