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AB133690

Anti-RRM1 antibody [EPR8482]

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(2 Publications)

Rabbit Recombinant Monoclonal RRM1 antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse, Human samples. Cited in 2 publications.

View Alternative Names

RR1, RRM1, Ribonucleoside-diphosphate reductase large subunit, Ribonucleoside-diphosphate reductase subunit M1, Ribonucleotide reductase large subunit

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM1 antibody [EPR8482] (AB133690)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM1 antibody [EPR8482] (AB133690)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling RRM1 with ab133690 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM1 antibody [EPR8482] (AB133690)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM1 antibody [EPR8482] (AB133690)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling RRM1 with ab133690 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-RRM1 antibody [EPR8482] (AB133690)
  • WB

Lab

Western blot - Anti-RRM1 antibody [EPR8482] (AB133690)

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/1000000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RRM1 antibody [EPR8482] (ab133690) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 4:

Rat colon tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 90 kDa,36 kDa

false

Exposure time: 10s

Western blot - Anti-RRM1 antibody [EPR8482] (AB133690)
  • WB

Lab

Western blot - Anti-RRM1 antibody [EPR8482] (AB133690)

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/1000000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

Lanes 1 - 4:

Western blot - Anti-RRM1 antibody [EPR8482] (ab133690) at 1/1000 dilution

Lanes 1 - 4:

Western blot - Anti-RRM1 antibody [EPR8482] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/rrm1-antibody-epr8482-bsa-and-azide-free-ab248620'>ab248620</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

Freshly made HeLa whole cell lysate at 20 µg

Lane 4:

Freshly made HEK-293 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 90 kDa,36 kDa

false

Exposure time: 10s

Western blot - Anti-RRM1 antibody [EPR8482] (AB133690)
  • WB

Unknown

Western blot - Anti-RRM1 antibody [EPR8482] (AB133690)

All lanes:

Western blot - Anti-RRM1 antibody [EPR8482] (ab133690) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HT29 cell lysate at 10 µg

Lane 3:

RAW 264.7 cell lysate at 10 µg

Lane 4:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 90 kDa

false

OI-RD Scanning - Anti-RRM1 antibody [EPR8482] (AB133690)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-RRM1 antibody [EPR8482] (AB133690)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-RRM1 antibody [EPR8482] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8482

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RRM1 also known as ribonucleotide reductase large subunit or RNR1 plays an important role in DNA synthesis and repair converting ribonucleotides to deoxyribonucleotides. This enzyme has a mass of approximately 90 kDa. RRM1 is found in multiple tissues exhibiting especially high expression levels in proliferative tissues and tumors. Its activity is tightly regulated through the cell cycle and it is essential during DNA replication and cell division.
Biological function summary

The large subunit of ribonucleotide reductase contributes to the enzymatic function through binding and activation of smaller subunits including RRM2 and RRM2B forming a complex. This complex is important for maintaining balanced deoxyribonucleotide pools during the S-phase of the cell cycle. Proper RRM1 function ensures the fidelity of DNA synthesis impacting genomic stability and cell viability.

Pathways

RRM1 acts within the DNA replication and repair pathways. It interacts with the tumor suppressor protein p53 which modulates its activities in response to DNA damage. Through pathways like the p53-dependent checkpoint RRM1 contributes to DNA damage response mechanisms. This interaction with p53 and involvement in the DNA replication pathway highlight its centrality in maintaining genomic integrity.

RRM1 has connections with cancer and chemoresistance. Overexpression of RRM1 often correlates with increased tumor aggressiveness and poor prognosis in several cancers including lung and pancreatic cancers. In the context of chemoresistance RRM1 interplays with the excision repair cross-complementation group 1 protein (ERCC1) impacting the effectiveness of certain chemotherapies. Its role in these conditions makes it a target for cancer diagnosis and treatment strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.
See full target information RRM1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancers 17: PubMed40227697

2025

Inducing Targeted, Caspase-Independent Apoptosis with New Chimeric Proteins for Treatment of Solid Cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Orly Melloul,Samar Zabit,Michal Lichtenstein,Deborah Duran,Myriam Grunewald,Haya Lorberboum-Galski

Scientific reports 14:13989 PubMed38886371

2024

In vitro evolution and whole genome analysis to study chemotherapy drug resistance in haploid human cells.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Carlos Jado,Michelle Dow,Krypton Carolino,Adam Klie,Gregory J Fonseca,Trey Ideker,Hannah Carter,Elizabeth A Winzeler
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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