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Rabbit Recombinant Monoclonal RRM1 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Cat, Dog, Rat, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human RRM1.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM1 antibody [HL1231] (AB308348), expandable thumbnail
  • Western blot - Anti-RRM1 antibody [HL1231] (AB308348), expandable thumbnail
  • Western blot - Anti-RRM1 antibody [HL1231] (AB308348), expandable thumbnail
  • Western blot - Anti-RRM1 antibody [HL1231] (AB308348), expandable thumbnail
  • Western blot - Anti-RRM1 antibody [HL1231] (AB308348), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human RRM1. The exact immunogen used to generate this antibody is proprietary information. Database link P23921

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBIHC-P
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Expected
Tested
Expected
Cat
Expected
Tested
Expected
Dog
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Expected
Expected

Species
Cat, Dog, Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Cat
Dilution info
1/500 - 1/3000
Notes

-

Species
Dog
Dilution info
1/500 - 1/3000
Notes

-

Species
Human
Dilution info
1/500 - 1/3000
Notes

-

Species
Rat
Dilution info
1/500 - 1/3000
Notes

-

Species
Mouse
Dilution info
1/500 - 1/3000
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Cat, Dog, Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.

Alternative names

RR1, RRM1, Ribonucleoside-diphosphate reductase large subunit, Ribonucleoside-diphosphate reductase subunit M1, Ribonucleotide reductase large subunit

Recommended products

Rabbit Recombinant Monoclonal RRM1 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Cat, Dog, Rat, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human RRM1.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human RRM1. The exact immunogen used to generate this antibody is proprietary information. Database link P23921
Carrier free
Yes
Clone number
HL1231
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

RRM1 also known as ribonucleotide reductase large subunit or RNR1 plays an important role in DNA synthesis and repair converting ribonucleotides to deoxyribonucleotides. This enzyme has a mass of approximately 90 kDa. RRM1 is found in multiple tissues exhibiting especially high expression levels in proliferative tissues and tumors. Its activity is tightly regulated through the cell cycle and it is essential during DNA replication and cell division.

Biological function summary

The large subunit of ribonucleotide reductase contributes to the enzymatic function through binding and activation of smaller subunits including RRM2 and RRM2B forming a complex. This complex is important for maintaining balanced deoxyribonucleotide pools during the S-phase of the cell cycle. Proper RRM1 function ensures the fidelity of DNA synthesis impacting genomic stability and cell viability.

Pathways

RRM1 acts within the DNA replication and repair pathways. It interacts with the tumor suppressor protein p53 which modulates its activities in response to DNA damage. Through pathways like the p53-dependent checkpoint RRM1 contributes to DNA damage response mechanisms. This interaction with p53 and involvement in the DNA replication pathway highlight its centrality in maintaining genomic integrity.

Associated diseases and disorders

RRM1 has connections with cancer and chemoresistance. Overexpression of RRM1 often correlates with increased tumor aggressiveness and poor prognosis in several cancers including lung and pancreatic cancers. In the context of chemoresistance RRM1 interplays with the excision repair cross-complementation group 1 protein (ERCC1) impacting the effectiveness of certain chemotherapies. Its role in these conditions makes it a target for cancer diagnosis and treatment strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM1 antibody [HL1231] (ab308348), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM1 antibody [HL1231] (ab308348)

    Paraffin-embedded human colon cancer tissue stained for RRM1 protein at cytoplasm with ab308348 at a 1/100 dilution in immunohistochemical analysis.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

  • Western blot - Anti-RRM1 antibody [HL1231] (ab308348), expandable thumbnail

    Western blot - Anti-RRM1 antibody [HL1231] (ab308348)

    Samples were seperated by 5% SDS-PAGE.

    All lanes: Western blot - Anti-RRM1 antibody [HL1231] (ab308348) at 1/1000 dilution

    Lane 1: Neuro2A whole cell extracts at 30 µg

    Lane 2: NIH-3T3 Whole cell extract at 30 µg

    Lane 3: Raw264.7 whole cell extract at 30 µg

    Lane 4: C2C12 whole cell extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 90 kDa

  • Western blot - Anti-RRM1 antibody [HL1231] (ab308348), expandable thumbnail

    Western blot - Anti-RRM1 antibody [HL1231] (ab308348)

    Samples were seperated by 5% SDS-PAGE.

    All lanes: Western blot - Anti-RRM1 antibody [HL1231] (ab308348) at 1/1000 dilution

    All lanes: Rat2 whole cell extracts at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 90 kDa

  • Western blot - Anti-RRM1 antibody [HL1231] (ab308348), expandable thumbnail

    Western blot - Anti-RRM1 antibody [HL1231] (ab308348)

    Separated by 5% SDS-PAGE.

    All lanes: Western blot - Anti-RRM1 antibody [HL1231] (ab308348) at 1/1000 dilution

    All lanes: HeLa cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 90.1 kDa

  • Western blot - Anti-RRM1 antibody [HL1231] (ab308348), expandable thumbnail

    Western blot - Anti-RRM1 antibody [HL1231] (ab308348)

    Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RRM1 antibody [HL1231] (ab308348) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-RRM1 antibody [HL1231] (ab308348) at 1/1000 dilution

    Lane 1: MDCK cell lysate at 30 µg

    Lane 2: PG-4 cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 90.1 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RRM1 antibody [HL1231] (ab308348), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RRM1 antibody [HL1231] (ab308348)

    For immunofluorescence analysis, HeLa cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of RRM1 protein using ab308348 at 1/500 dilution (green). Red: alpha Tubulin, a cytoskeleton marker. Nuclei (blue) is stained with fluoroshield with DAPI.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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