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Mouse Monoclonal RRM2 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 38 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RRM2 aa 1-150.


Images

Western blot - Anti-RRM2 antibody [1E1] (AB57653), expandable thumbnail
  • Flow Cytometry - Anti-RRM2 antibody [1E1] (AB57653), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM2 antibody [1E1] (AB57653), expandable thumbnail
  • Western blot - Anti-RRM2 antibody [1E1] (AB57653), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-RRM2 antibody [1E1] (AB57653), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human RRM2 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link P31350

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBIHC-PICC/IF
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling.

Alternative names

RR2, RRM2, Ribonucleoside-diphosphate reductase subunit M2, Ribonucleotide reductase small chain, Ribonucleotide reductase small subunit

Recommended products

Mouse Monoclonal RRM2 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 38 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RRM2 aa 1-150.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human RRM2 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link P31350
Clone number
1E1
Purification technique
Affinity purification Protein A
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product was changed from ascites to tissue culture supernatant on 12/3/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

RRM2 also known as Ribonucleotide Reductase Regulatory Subunit M2 is an important component of the ribonucleotide reductase enzyme complex which plays a significant part in DNA synthesis. This protein helps convert ribonucleotides into deoxyribonucleotides. RRM2 is approximately 45 kDa in size. Its expression occurs mostly in proliferating cells with high levels detected in the cytoplasm and nucleus. The expression level of RRM2 can vary significantly across different tissues correlating closely with cell division rates.

Biological function summary

The ribonucleotide reductase enzyme facilitates the critical function of DNA replication and repair. RRM2 forms a complex with RRM1 the large subunit necessary for enzyme activity. This complex regulates the balance of deoxyribonucleotide pools necessary for DNA synthesis. Variations or dysregulations in the activity of RRM2 can influence genetic stability and cell proliferation emphasizing its importance in cellular function.

Pathways

Ribonucleotide reductase and RRM2 participate significantly in the DNA synthesis and repair pathway. It is pivotal for maintaining DNA fidelity during replication. RRM2 actions relate to pathways involving other DNA replication proteins such as RRM1 and the replication factor C family. The synthesis pathway necessitates close cooperation between these proteins to ensure proper DNA replication and cell cycle progression.

Associated diseases and disorders

Research has linked RRM2 to cancer and viral infections. Overexpression or mutations in the RRM2 gene may contribute to cancer development as seen in various tumor types indicating it as a potential target for cancer therapeutics. Moreover some viruses may hijack the host's RRM2 to facilitate their own replication. Targeting the RRM2 protein could potentially lead to novel treatment approaches in these diseases connecting it with other proteins like RRM1 which also share roles in tumor progression and viral replication.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-RRM2 antibody [1E1] (ab57653), expandable thumbnail

    Western blot - Anti-RRM2 antibody [1E1] (ab57653)

    This image was generated using the ascites version of the product.

    All lanes: Western blot - Anti-RRM2 antibody [1E1] (ab57653) at 1 µg/mL

    All lanes: HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

    Predicted band size: 45 kDa

  • Flow Cytometry - Anti-RRM2 antibody [1E1] (ab57653), expandable thumbnail

    Flow Cytometry - Anti-RRM2 antibody [1E1] (ab57653)

    Overlay histogram showing HepG2 cells stained with ab57653 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57653, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This image was generated using the ascites version of the product.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM2 antibody [1E1] (ab57653), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM2 antibody [1E1] (ab57653)

    RRM2 antibody (ab57653) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human stomach.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-RRM2 antibody [1E1] (ab57653), expandable thumbnail

    Western blot - Anti-RRM2 antibody [1E1] (ab57653)

    RRM2 antibody (ab57653) at 1ug/lane + HeLa cell lysate at 25ug/lane.

    This image was generated using the ascites version of the product.

    All lanes: Western blot - Anti-RRM2 antibody [1E1] (ab57653)

    Predicted band size: 45 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RRM2 antibody [1E1] (ab57653), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RRM2 antibody [1E1] (ab57653)

    ICC/IF image of ab57653 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57653, 10μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
    This image was generated using the ascites version of the product.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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