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Rabbit Recombinant Monoclonal RRM2 antibody. Carrier free. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human samples.

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Images

Western blot - Anti-RRM2 antibody [EPR11820] - BSA and Azide free (AB240166), expandable thumbnail
  • Immunoprecipitation - Anti-RRM2 antibody [EPR11820] - BSA and Azide free (AB240166), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-RRM2 antibody [EPR11820] - BSA and Azide free (AB240166), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM2 antibody [EPR11820] - BSA and Azide free (AB240166), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM2 antibody [EPR11820] - BSA and Azide free (AB240166), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Tested
Not recommended
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

1 product for Alternative Version

Target data

Function

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling.

Alternative names

RR2, RRM2, Ribonucleoside-diphosphate reductase subunit M2, Ribonucleotide reductase small chain, Ribonucleotide reductase small subunit

Recommended products

Rabbit Recombinant Monoclonal RRM2 antibody. Carrier free. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR11820
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab240166 is the carrier-free version of Anti-RRM2 antibody [EPR11820] ab172476.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

RRM2 also known as Ribonucleotide Reductase Regulatory Subunit M2 is an important component of the ribonucleotide reductase enzyme complex which plays a significant part in DNA synthesis. This protein helps convert ribonucleotides into deoxyribonucleotides. RRM2 is approximately 45 kDa in size. Its expression occurs mostly in proliferating cells with high levels detected in the cytoplasm and nucleus. The expression level of RRM2 can vary significantly across different tissues correlating closely with cell division rates.

Biological function summary

The ribonucleotide reductase enzyme facilitates the critical function of DNA replication and repair. RRM2 forms a complex with RRM1 the large subunit necessary for enzyme activity. This complex regulates the balance of deoxyribonucleotide pools necessary for DNA synthesis. Variations or dysregulations in the activity of RRM2 can influence genetic stability and cell proliferation emphasizing its importance in cellular function.

Pathways

Ribonucleotide reductase and RRM2 participate significantly in the DNA synthesis and repair pathway. It is pivotal for maintaining DNA fidelity during replication. RRM2 actions relate to pathways involving other DNA replication proteins such as RRM1 and the replication factor C family. The synthesis pathway necessitates close cooperation between these proteins to ensure proper DNA replication and cell cycle progression.

Associated diseases and disorders

Research has linked RRM2 to cancer and viral infections. Overexpression or mutations in the RRM2 gene may contribute to cancer development as seen in various tumor types indicating it as a potential target for cancer therapeutics. Moreover some viruses may hijack the host's RRM2 to facilitate their own replication. Targeting the RRM2 protein could potentially lead to novel treatment approaches in these diseases connecting it with other proteins like RRM1 which also share roles in tumor progression and viral replication.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-RRM2 antibody [EPR11820] - BSA and Azide free (ab240166), expandable thumbnail

    Western blot - Anti-RRM2 antibody [EPR11820] - BSA and Azide free (ab240166)

    This data was developed using Anti-RRM2 antibody [EPR11820] ab172476, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-RRM2 antibody [EPR11820] (Anti-RRM2 antibody [EPR11820] ab172476) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: HepG2 cell lysate at 10 µg

    Lane 3: K562 cell lysate at 10 µg

    Lane 4: Jurkat cell lysate at 10 µg

    Predicted band size: 45 kDa

  • Immunoprecipitation - Anti-RRM2 antibody [EPR11820] - BSA and Azide free (ab240166), expandable thumbnail

    Immunoprecipitation - Anti-RRM2 antibody [EPR11820] - BSA and Azide free (ab240166)

    Western blot analysis on immunoprecipitation pellet from HeLa cell lysate (lane 1) labeling RRM2 with Anti-RRM2 antibody [EPR11820] ab172476 at 1/10 dilution or 1X PBS (lane 2) negative control. HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RRM2 antibody [EPR11820] ab172476).

    All lanes: Immunoprecipitation - Anti-RRM2 antibody [EPR11820] (Anti-RRM2 antibody [EPR11820] ab172476)

    Predicted band size: 45 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RRM2 antibody [EPR11820] - BSA and Azide free (ab240166), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RRM2 antibody [EPR11820] - BSA and Azide free (ab240166)

    Immunofluorescent analysis of HeLa cells labeling RRM2 with Anti-RRM2 antibody [EPR11820] ab172476 at 1/100 dilution (red). DAPI nuclear staining (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RRM2 antibody [EPR11820] ab172476).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM2 antibody [EPR11820] - BSA and Azide free (ab240166), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM2 antibody [EPR11820] - BSA and Azide free (ab240166)

    Immunohistochemical analysis of Paraffin-embedded sections Human Cerebrum tissue labelling RRM2 with Anti-RRM2 antibody [EPR11820] ab172476 at 1/1000 dilution, followed by LeicaDS9800 (Bond® Polymer Refine Detection)(Ready to use). Negative/low expression control: no staining in human cerebrum (PMID: 30745840). The section was incubated with Anti-RRM2 antibody [EPR11820] ab172476 for 30 mins at room temperature. Counterstained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond® Polymer Refine Detection)(Ready to use). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins

    This data was developed using Anti-RRM2 antibody [EPR11820] ab172476, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM2 antibody [EPR11820] - BSA and Azide free (ab240166), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM2 antibody [EPR11820] - BSA and Azide free (ab240166)

    Immunohistochemical analysis of Paraffin-embedded sections Human Colon carcinoma tissue labelling RRM2 with Anti-RRM2 antibody [EPR11820] ab172476 at 1/1000 dilution, followed by LeicaDS9800 (Bond® Polymer Refine Detection)(Ready to use). Positive staining on Human Colon carcinoma is observed. The section was incubated with Anti-RRM2 antibody [EPR11820] ab172476 for 30 mins at room temperature. Counterstained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond® Polymer Refine Detection)(Ready to use).

    Positive staining on human colon carcinoma. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins.

    This data was developed using Anti-RRM2 antibody [EPR11820] ab172476, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM2 antibody [EPR11820] - BSA and Azide free (ab240166), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM2 antibody [EPR11820] - BSA and Azide free (ab240166)

    Immunohistochemical analysis of Paraffin-embedded sections Human Stomach tissue labelling RRM2 with Anti-RRM2 antibody [EPR11820] ab172476 at 1/1000 dilution, followed by LeicaDS9800 (Bond® Polymer Refine Detection)(Ready to use).Human stomach staining on Human Stomach is observed. Counterstained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond® Polymer Refine Detection)(Ready to use).

    Positive staining in human stomach. The section was incubated with Anti-RRM2 antibody [EPR11820] ab172476 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins.

    This data was developed using Anti-RRM2 antibody [EPR11820] ab172476, the same antibody clone in a different buffer formulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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