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AB308351

Anti-RRM2 antibody [HL1235] - BSA and Azide free

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Rabbit Recombinant Monoclonal RRM2 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Zebrafish samples. Immunogen corresponding to Recombinant Fragment Protein within Human RRM2.

View Alternative Names

RR2, RRM2, Ribonucleoside-diphosphate reductase subunit M2, Ribonucleotide reductase small chain, Ribonucleotide reductase small subunit

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-RRM2 antibody [HL1235] - BSA and Azide free (AB308351)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RRM2 antibody [HL1235] - BSA and Azide free (AB308351)

For immunofluorescence analysis, HeLa cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of RRM2 protein using ab308351 at 1/500 dilution (green). Nuclei (blue) is stained with fluoroshield with DAPI

Western blot - Anti-RRM2 antibody [HL1235] - BSA and Azide free (AB308351)
  • WB

Supplier Data

Western blot - Anti-RRM2 antibody [HL1235] - BSA and Azide free (AB308351)

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RRM2 antibody [HL1235] (ab308351) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-RRM2 antibody [HL1235] - BSA and Azide free (ab308351) at 1/1000 dilution

Lane 1:

RMS13 cell lysate at 30 µg

Lane 2:

THP1 cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 44.9 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM2 antibody [HL1235] - BSA and Azide free (AB308351)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM2 antibody [HL1235] - BSA and Azide free (AB308351)

Immunohistochemical analysis of paraffin-embedded 2 days-post-fertilization zebrafish embryo labelling RRM2 with ab308351 at 1/100 dilution.

Antigen Retrieval : Tris-HCl buffer, pH 9.0, 20 min at 70°C

Western blot - Anti-RRM2 antibody [HL1235] - BSA and Azide free (AB308351)
  • WB

Supplier Data

Western blot - Anti-RRM2 antibody [HL1235] - BSA and Azide free (AB308351)

Separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-RRM2 antibody [HL1235] - BSA and Azide free (ab308351) at 1/1000 dilution

All lanes:

Whole zebrafish lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 44.9 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1235

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Zebrafish

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human RRM2. The exact immunogen used to generate this antibody is proprietary information.

P31350

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Complementary Products

Primary Antibodies

AB137114

Anti-RRM1 antibody [EPR8483]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM1 antibody [EPR8483] (AB137114)

  • 5
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
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Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB87277

Anti-RPA32/RPA2 (phospho S4 + S8) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 (phospho S4 + S8) antibody (AB87277)

  • 4
  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RRM2 also known as Ribonucleotide Reductase Regulatory Subunit M2 is an important component of the ribonucleotide reductase enzyme complex which plays a significant part in DNA synthesis. This protein helps convert ribonucleotides into deoxyribonucleotides. RRM2 is approximately 45 kDa in size. Its expression occurs mostly in proliferating cells with high levels detected in the cytoplasm and nucleus. The expression level of RRM2 can vary significantly across different tissues correlating closely with cell division rates.
Biological function summary

The ribonucleotide reductase enzyme facilitates the critical function of DNA replication and repair. RRM2 forms a complex with RRM1 the large subunit necessary for enzyme activity. This complex regulates the balance of deoxyribonucleotide pools necessary for DNA synthesis. Variations or dysregulations in the activity of RRM2 can influence genetic stability and cell proliferation emphasizing its importance in cellular function.

Pathways

Ribonucleotide reductase and RRM2 participate significantly in the DNA synthesis and repair pathway. It is pivotal for maintaining DNA fidelity during replication. RRM2 actions relate to pathways involving other DNA replication proteins such as RRM1 and the replication factor C family. The synthesis pathway necessitates close cooperation between these proteins to ensure proper DNA replication and cell cycle progression.

Research has linked RRM2 to cancer and viral infections. Overexpression or mutations in the RRM2 gene may contribute to cancer development as seen in various tumor types indicating it as a potential target for cancer therapeutics. Moreover some viruses may hijack the host's RRM2 to facilitate their own replication. Targeting the RRM2 protein could potentially lead to novel treatment approaches in these diseases connecting it with other proteins like RRM1 which also share roles in tumor progression and viral replication.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling.
See full target information RRM2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com