AB112052

Anti-RRN3 antibody

Name: Anti-RRN3 antibody (ab112052) | Abcam
Brand: Abcam Limited
SKU: AB112052
Price: 520 USD
Availability: InStock

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(7 Publications)

Rabbit Polyclonal RRN3 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human RRN3 aa 600 to C-terminus.

View Alternative Names

TIFIA, RRN3, RNA polymerase I-specific transcription initiation factor RRN3, Transcription initiation factor IA, TIF-IA

2 Images

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Immunoprecipitation - Anti-RRN3 antibody (AB112052)

ab112052 at 1 µg/ml staining RRN3 in HeLa whole cell lysate immunoprecipitated using ab112052 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2 : controle IgG. Predicted band size : 74 kDa Exposure time : 10 seconds

All lanes:

Immunoprecipitation - Anti-RRN3 antibody (ab112052)

Predicted band size: 74 kDa

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Western blot - Anti-RRN3 antibody (AB112052)

All lanes:

Western blot - Anti-RRN3 antibody (ab112052) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 74 kDa

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Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human RRN3 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NYV6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The RRN3 protein also known as TIF-IA plays an important role in the initiation of ribosomal RNA (rRNA) synthesis. It weighs approximately 70 kDa and is present mainly in the nucleolus of cells where ribosome production takes place. RRN3 functions as a transcription factor and interacts with RNA polymerase I facilitating the transcription of rRNA genes. It is an essential player in cellular growth and proliferation due to its role in ribosome biogenesis which is a fundamental process in all active eukaryotic cells.

Biological function summary

RRN3 is involved in the regulation of ribosome production. It acts as a mediator between the RNA polymerase I complex and the transcription machinery ensuring accurate transcription initiation of rRNA. This interaction forms part of a larger complex that includes the upstream binding factor (UBF) which stabilizes the pre-initiation complex promoting efficient rRNA transcription. RRN3 is important for the maintenance of cellular metabolic activity and overall protein synthesis levels.

Pathways

RRN3 is integral to nutrient signaling and cell growth regulation. It participates in the mechanistic target of rapamycin (mTOR) pathway which controls cell growth and proliferation in response to nutrient availability. RRN3 interacts with mTORC1 to link nutrient signals to ribosome production modulating cellular growth rates. Furthermore RRN3 is associated with the ribosomal biogenesis stress response pathway engaging proteins such as S6 kinase which is activated under conditions requiring increased protein synthesis.

RRN3 is connected to cancer and ribosomopathies. Overexpression of RRN3 has been observed in various cancers suggesting its role in uncontrolled cell proliferation characteristic of tumor growth. Cancerous cells frequently have increased ribosome biogenesis to support rapid cell division with RRN3 serving as a critical factor in this process. Additionally disruptions in RRN3 function can affect ribosomopathies disorders caused by defects in ribosome production. Interactions with proteins like MDM2 which regulates the tumor suppressor protein p53 highlight the involvement of RRN3 in these diseases further emphasizing its importance in cellular regulation and disease onset.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for efficient transcription initiation by RNA polymerase I (Pol I). Required for the formation of the competent pre-initiation complex (PIC).

See full target information RRN3

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e23167 PubMed38169774

2024

Inhibition of TAF1B impairs ribosome biosynthesis and suppresses cell proliferation in stomach adenocarcinoma through promoting c-MYC mRNA degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Hang-Fei Chen,Zhang-Ping Li,Qi Wu,Chun Yu,Jing-Yi Yan,Yong-Feng Bai,Sheng-Mei Zhu,Mao-Xiang Qian,Ming Liu,Li-Feng Xu,Zheng Peng,Feng Zhang

The Journal of biological chemistry 294:19155-19166 PubMed31694914

2019

SUMOylation down-regulates rDNA transcription by repressing expression of upstream-binding factor and proto-oncogene c-Myc.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Peng,Zhenxing Wang,Zhiqiang Wang,Fang Yu,Jiwen Li,Jiemin Wong

Muscle & nerve 58:277-285 PubMed29572878

2018

Muscle contractures in patients with cerebral palsy and acquired brain injury are associated with extracellular matrix expansion, pro-inflammatory gene expression, and reduced rRNA synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ferdinand Von Walden,Stefan Gantelius,Chang Liu,Hanna Borgström,Lars Björk,Ola Gremark,Per Stål,Gustavo A Nader,Eva PontéN

Cell reports 23:404-414 PubMed29642000

2018

Small-Molecule Targeting of RNA Polymerase I Activates a Conserved Transcription Elongation Checkpoint.

Applications

Species

Unspecified reactive species

Ting Wei,Saman M Najmi,Hester Liu,Karita Peltonen,Alena Kucerova,David A Schneider,Marikki Laiho

Molecular and cellular biology 37: PubMed28874518

2017

Internal Associations of the Acidic Region of Upstream Binding Factor Control Its Nucleolar Localization.

Applications

Unspecified application

Species

Unspecified reactive species

Shuhei Ueshima,Kyosuke Nagata,Mitsuru Okuwaki

Molecular and cellular biology 35:4170-84 PubMed26416883

2015

Mild Glucose Starvation Induces KDM2A-Mediated H3K36me2 Demethylation through AMPK To Reduce rRNA Transcription and Cell Proliferation.

Applications

ChIP

Species

Human

Yuji Tanaka,Hirohisa Yano,Sachiko Ogasawara,Sho-Ichi Yoshioka,Hiromi Imamura,Kengo Okamoto,Makoto Tsuneoka

Biochemical and biophysical research communications 443:22-7 PubMed24269811

2013

Upstream binding factor-dependent and pre-rRNA transcription-independent association of pre-rRNA processing factors with rRNA gene.

Applications

Unspecified application

Species

Unspecified reactive species

Shuhei Ueshima,Kyosuke Nagata,Mitsuru Okuwaki

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RRN3 antibody

Immunoprecipitation - Anti-RRN3 antibody (AB112052)

Western blot - Anti-RRN3 antibody (AB112052)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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