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AB224105

Anti-RRP1B antibody

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(2 Publications)

Rabbit Polyclonal RRP1B antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RRP1B aa 300-500.

View Alternative Names

KIAA0179, RRP1B, Ribosomal RNA processing protein 1 homolog B, RRP1-like protein B

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRP1B antibody (AB224105)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRP1B antibody (AB224105)

Immunohistochemistry analysis of human skin tissue labeling RRP1B with ab224105 at 1 : 1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRP1B antibody (AB224105)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRP1B antibody (AB224105)

Immunohistochemistry analysis of human cerebellum tissue labeling RRP1B with ab224105 at 1 : 1000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-RRP1B antibody (AB224105)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RRP1B antibody (AB224105)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for RRP1B (green) using ab224105 (4 μg/ml) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRP1B antibody (AB224105)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRP1B antibody (AB224105)

Immunohistochemistry analysis of human testis tissue labeling RRP1B with ab224105 at 1 : 1000 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human RRP1B aa 300-500. The exact immunogen used to generate this antibody is proprietary information.

Q14684

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RRP1B also known as Ribosomal RNA Processing 1B is a protein involved in the processing of pre-rRNA. It has a molecular mass of approximately 85 kDa. RRP1B is widely expressed in human tissues including high expression levels in the nucleus of cells. As its name implies RRP1B interacts with components involved in the maturation of rRNA and plays a role in the assembly and modification of the nucleolus.
Biological function summary

RRP1B engages in chromatin remodeling and modulates the expression of genes. It is part of a complex responsible for nucleolar rRNA processing. This protein is not only important for rRNA maturation but also influences the transcriptional regulation of several genes through chromatin reorganization. These activities highlight its involvement in both gene expression regulation and cellular structural organization.

Pathways

RRP1B significantly impacts cellular pathways related to ribosome biogenesis and chromatin modification. In the ribosome biogenesis pathway it collaborates with proteins such as NOP52 and NOP56 to ensure proper rRNA processing and ribosome assembly. Additionally RRP1B interacts within the chromatin-modifying pathway coordinating with proteins like CHD and SW1/SNF complexes to regulate gene expression.

RRP1B has links to cancers including breast cancer. Alterations in RRP1B expression or function can influence cancer progression as it may affect metastasis and chromatin organization. Researchers also study interactions between RRP1B and proteins such as BRCA1 which is involved in DNA repair processes to understand its potential impact on tumor development and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Positively regulates DNA damage-induced apoptosis by acting as a transcriptional coactivator of proapoptotic target genes of the transcriptional activator E2F1 (PubMed : 20040599). Likely to play a role in ribosome biogenesis by targeting serine/threonine protein phosphatase PP1 to the nucleolus (PubMed : 20926688). Involved in regulation of mRNA splicing (By similarity). Inhibits SIPA1 GTPase activity (By similarity). Involved in regulating expression of extracellular matrix genes (By similarity). Associates with chromatin and may play a role in modulating chromatin structure (PubMed : 19710015).. (Microbial infection) Following influenza A virus (IAV) infection, promotes viral mRNA transcription by facilitating the binding of IAV RNA-directed RNA polymerase to capped mRNA.
See full target information RRP1B
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 7:1649 PubMed39702755

2024

Methylation-modulated PFTK1 regulates gefitinib resistance via Wnt/β-catenin signaling in EGFR mutant non-small-cell lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoting Jia,Jingjie Tian,Pingping Chen,Jing Dong,Lei Li,Danyang Chen,Jianlei Zhang,Dongjiang Liao,Zhimin He,Kai Luo

Cell reports 41:111726 PubMed36450254

2022

The ribosomal RNA processing 1B:protein phosphatase 1 holoenzyme reveals non-canonical PP1 interaction motifs.

Applications

Unspecified application

Species

Unspecified reactive species

Gautam Srivastava,Rakhi Bajaj,Ganesan Senthil Kumar,Antoine Gaudreau-Lapierre,Hannah Nicolas,Delphine Chamousset,Dale Kreitler,Wolfgang Peti,Laura Trinkle-Mulcahy,Rebecca Page
View all publications
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Product promise

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