AB137250

Anti-RRP41 antibody

Name: Anti-RRP41 antibody (ab137250) | Abcam
Brand: Abcam Limited
SKU: AB137250
Price: 535 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal RRP41 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human EXOSC4 aa 1-50.

View Alternative Names

RRP41, SKI6, EXOSC4, Exosome complex component RRP41, Exosome component 4, Ribosomal RNA-processing protein 41, p12A

2 Images

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Immunoprecipitation - Anti-RRP41 antibody (AB137250)

Immunoprecipitation with ab137250 at 6μg/mg lysate. 1 mg of HeLa whole cell lysate used for IP (20% of IP loaded). Western blot labelling RRP41 with ab137250 at 1 μg/ml. Detected by chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-RRP41 antibody (ab137250)

Predicted band size: 112 kDa,26 kDa,37 kDa,60 kDa

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Western blot - Anti-RRP41 antibody (AB137250)

All lanes:

Western blot - Anti-RRP41 antibody (ab137250) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 26 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human EXOSC4 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9NPD3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab137250 was affinity purified using an epitope specific to RRP41 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RRP41 also known as EXOSC4 is a subunit of the RNA exosome complex. It is involved in the degradation and processing of RNA. With molecular mass approximately 27 kDa RRP41 partakes in catalytic core activities within the exosome. Scientists have identified its presence mainly in the nucleus and cytoplasm highlighting its broad cellular expression.

Biological function summary

RRP41 participates in RNA metabolism by aiding in the degradation of various RNA species. It is a component of the exosome complex which consists of several other subunits working together to process RNA. This complex is vital in maintaining RNA homeostasis allowing cells to regulate gene expression effectively. RRP41's role in the exosome ensures proper processing of ribosomal RNA (rRNA) messenger RNA (mRNA) and other RNA molecules.

Pathways

RRP41 functions within the RNA surveillance pathway. This pathway helps identify and eliminate defective RNA therefore ensuring RNA quality control within the cell. Additionally it interacts with other proteins such as RRP42 and RRP43 which are also part of the exosome complex. The interaction between these proteins is important in regulating RNA degradation and processing functions safeguarding the precision of cellular activities.

RRP41 displays connections to autoimmune diseases such as systemic lupus erythematosus (SLE). Misregulation of exosome functions including RRP41 might contribute to the improper clearance of RNA leading to disease development. Furthermore interactions with proteins like RRP45 which also play roles in RNA processing suggest RRP41 involvement in the pathophysiology of certain RNA-related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC4 binds to ARE-containing RNAs.

See full target information EXOSC4

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 38:110349 PubMed35139382

2022

PD-L1 promotes myofibroblastic activation of hepatic stellate cells by distinct mechanisms selective for TGF-β receptor I versus II.

Applications

Unspecified application

Species

Unspecified reactive species

Liankang Sun,Yuanguo Wang,Xianghu Wang,Amaia Navarro-Corcuera,Sumera Ilyas,Nidhi Jalan-Sakrikar,Can Gan,Xinyi Tu,Yu Shi,Kangsheng Tu,Qingguang Liu,Zhenkun Lou,Haidong Dong,Arlene H Sharpe,Vijay H Shah,Ningling Kang

eLife 10: PubMed34085923

2021

Cytoplasmic mRNA decay represses RNA polymerase II transcription during early apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher Duncan-Lewis,Ella Hartenian,Valeria King,Britt A Glaunsinger

Molecular cell 77:1340-1349.e6 PubMed32006463

2020

Extraction of mRNA from Stalled Ribosomes by the Ski Complex.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra Zinoviev,Rustam K Ayupov,Irina S Abaeva,Christopher U T Hellen,Tatyana V Pestova

The Journal of biological chemistry 291:15958-74 PubMed27281821

2016

RNA Exosome Complex Regulates Stability of the Hepatitis B Virus X-mRNA Transcript in a Non-stop-mediated (NSD) RNA Quality Control Mechanism.

Applications

Species

Human

Hussein H Aly,Junya Suzuki,Koichi Watashi,Kazuaki Chayama,Shin-Ichi Hoshino,Makoto Hijikata,Takanobu Kato,Takaji Wakita

Molecular cell 60:873-85 PubMed26687677

2015

DGCR8 Acts as an Adaptor for the Exosome Complex to Degrade Double-Stranded Structured RNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Macias,Ross A Cordiner,Philippe Gautier,Mireya Plass,Javier F Cáceres

Biochemical Society transactions 41:838-43 PubMed23863141

2013

Cellular functions of the microprocessor.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Macias,Ross A Cordiner,Javier F Cáceres

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-RRP41 antibody

Immunoprecipitation - Anti-RRP41 antibody (AB137250)

Western blot - Anti-RRP41 antibody (AB137250)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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