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Rabbit Recombinant Monoclonal RRP42 antibody. Suitable for IHC-P, WB and reacts with Human samples.


Images

Western blot - Anti-RRP42 antibody [EPR7452] (AB126750), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRP42 antibody [EPR7452] (AB126750), expandable thumbnail
  • OI-RD Scanning - Anti-RRP42 antibody [EPR7452] (AB126750), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFFlow CytWB
Human
Tested
Not recommended
Not recommended
Tested

Tested
Tested

Species

Human

Dilution info

1/100 - 1/250

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/10000 - 1/50000

Notes

-

Target data

Function

Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal RRP42 antibody. Suitable for IHC-P, WB and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR7452

Purification technique

Affinity purification Protein A

Dissociation constant

1.9 x 10-11 M

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

RRP42 also known as EXOSC7 is a component of the RNA exosome complex. It weighs around 28 kDa. This protein is found mainly in the nucleolus and cytoplasm where it helps with RNA processing and degradation. RRP42 acts as a scaffold within the exosome complex playing a role in structuring the exosome for interactions with other proteins and RNA substrates.

Biological function summary

RRP42 forms part of the core of the exosome complex which is essential for maintaining RNA homeostasis. This exosome complex includes several other proteins like RRP4 RRP40 and EXOSC10 creating a multiprotein assembly that processes a variety of RNA species. Through these interactions RRP42 participates in the degradation and maturation of different types of RNA like ribosomal RNA messenger RNA and small nuclear RNA.

Pathways

RRP42's role in the exosome relates to RNA metabolic processes and related pathways. It engages in the RNA processing pathway where it works on properly modifying RNA molecules. Another pathway where RRP42 is active is the surveillance pathway that protects the cell from faulty RNA. In these roles it associates with other proteins such as SKIV2L and DIS3 which also contribute to RNA turnover and quality control.

Associated diseases and disorders

The activity of RRP42 influences conditions related to RNA processing defects. Improper function or regulation of RRP42 may associate with specific genetic disorders like pontocerebellar hypoplasia and some cancers where RNA metabolism becomes disrupted. Connections to these diseases often involve interactions with proteins like EXOSC3 and EXOSC8 which are part of the same exosome complex and contribute to the disease phenotypes through similar mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-RRP42 antibody [EPR7452] (ab126750), expandable thumbnail

    Western blot - Anti-RRP42 antibody [EPR7452] (ab126750)

    All lanes: Western blot - Anti-RRP42 antibody [EPR7452] (ab126750) at 1/10000 dilution

    Lane 1: K562 cell lysate at 10 µg

    Lane 2: Jurkat cell lysate at 10 µg

    Lane 3: NCI-H460 cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 32 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRP42 antibody [EPR7452] (ab126750), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRP42 antibody [EPR7452] (ab126750)

    ab126750, at 1/100 dilution, staining RRP42 in paraffin-embedded Human colon tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-RRP42 antibody [EPR7452] (ab126750), expandable thumbnail

    OI-RD Scanning - Anti-RRP42 antibody [EPR7452] (ab126750)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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