Rabbit Recombinant Monoclonal RRP42 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
IHC-P | ICC/IF | Flow Cyt | WB | |
---|---|---|---|---|
Human | Tested | Not recommended | Not recommended | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes.
KIAA0116, RRP42, RRP42, KIAA0116, EXOSC7, Exosome complex component RRP42, Exosome component 7, Ribosomal RNA-processing protein 42, p8
Rabbit Recombinant Monoclonal RRP42 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
EPR7452
Affinity purification Protein A
1.9 x 10-11 M
Blue Ice
+4°C
+4°C
Do Not Freeze
ab248166 is the carrier-free version of Anti-RRP42 antibody [EPR7452] ab126750.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
RRP42 also known as EXOSC7 is a component of the RNA exosome complex. It weighs around 28 kDa. This protein is found mainly in the nucleolus and cytoplasm where it helps with RNA processing and degradation. RRP42 acts as a scaffold within the exosome complex playing a role in structuring the exosome for interactions with other proteins and RNA substrates.
RRP42 forms part of the core of the exosome complex which is essential for maintaining RNA homeostasis. This exosome complex includes several other proteins like RRP4 RRP40 and EXOSC10 creating a multiprotein assembly that processes a variety of RNA species. Through these interactions RRP42 participates in the degradation and maturation of different types of RNA like ribosomal RNA messenger RNA and small nuclear RNA.
RRP42's role in the exosome relates to RNA metabolic processes and related pathways. It engages in the RNA processing pathway where it works on properly modifying RNA molecules. Another pathway where RRP42 is active is the surveillance pathway that protects the cell from faulty RNA. In these roles it associates with other proteins such as SKIV2L and DIS3 which also contribute to RNA turnover and quality control.
The activity of RRP42 influences conditions related to RNA processing defects. Improper function or regulation of RRP42 may associate with specific genetic disorders like pontocerebellar hypoplasia and some cancers where RNA metabolism becomes disrupted. Connections to these diseases often involve interactions with proteins like EXOSC3 and EXOSC8 which are part of the same exosome complex and contribute to the disease phenotypes through similar mechanisms.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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Terms & Conditions.
This data was developed using Anti-RRP42 antibody [EPR7452] ab126750, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-RRP42 antibody [EPR7452] (Anti-RRP42 antibody [EPR7452] ab126750) at 1/10000 dilution
Lane 1: K562 cell lysate at 10 µg
Lane 2: Jurkat cell lysate at 10 µg
Lane 3: NCI-H460 cell lysate at 10 µg
All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 32 kDa
This data was developed using Anti-RRP42 antibody [EPR7452] ab126750, the same antibody clone in a different buffer formulation.Anti-RRP42 antibody [EPR7452] ab126750, at 1/100 dilution, staining RRP42 in paraffin-embedded Human colon tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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