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AB250823

Anti-RRP7A antibody [EPR14412] - BSA and Azide free

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Rabbit Recombinant Monoclonal RRP7A antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.

View Alternative Names

CGI-96, RRP7A, Ribosomal RNA-processing protein 7 homolog A, Gastric cancer antigen Zg14

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-RRP7A antibody [EPR14412] - BSA and Azide free (AB250823)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RRP7A antibody [EPR14412] - BSA and Azide free (AB250823)

This data was developed using ab185225, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 293T cells (4% paraformaldehyde-fixed) labeling RRP7A with ab185225 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Western blot - Anti-RRP7A antibody [EPR14412] - BSA and Azide free (AB250823)
  • WB

Supplier Data

Western blot - Anti-RRP7A antibody [EPR14412] - BSA and Azide free (AB250823)

This data was developed using ab185225, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RRP7A antibody [EPR14412] - C-terminal (<a href='/en-us/products/primary-antibodies/rrp7a-antibody-epr14412-c-terminal-ab185225'>ab185225</a>) at 1/1000 dilution

All lanes:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 32 kDa

false

Western blot - Anti-RRP7A antibody [EPR14412] - BSA and Azide free (AB250823)
  • WB

Supplier Data

Western blot - Anti-RRP7A antibody [EPR14412] - BSA and Azide free (AB250823)

This data was developed using ab185225, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RRP7A antibody [EPR14412] - C-terminal (<a href='/en-us/products/primary-antibodies/rrp7a-antibody-epr14412-c-terminal-ab185225'>ab185225</a>) at 1/5000 dilution

Lane 1:

MCF7 cell lysate at 20 µg

Lane 2:

293T cell lysate at 20 µg

Lane 3:

A431 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14412

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab250823 is the carrier-free version of ab185225.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RRP7A also known as Ribosomal RNA Processing 7 Homolog A is a protein involved in the assembly of ribosomal subunits. It has a molecular mass of approximately 33 kDa. RRP7A localizes mainly in the nucleolus where ribosomal RNA (rRNA) synthesis and ribosome assembly occur. It exists in various organisms indicating its conserved function across species. The protein is encoded by the RRP7A gene and its expression is observed in many tissues reflecting its essential function in the basic cellular process of ribosome production.
Biological function summary

RRP7A plays an important role in ribosome biogenesis an essential process for the synthesis of proteins. It is part of the small-subunit (SSU) processome complex which is involved in the maturation of 18S rRNA a critical component of the small ribosomal subunit. The SSU processome is essential for early steps of 40S ribosomal subunit assembly. RRP7A acts in association with other proteins within this complex to facilitate the accurate processing of pre-rRNA into functional rRNA molecules necessary for proper ribosomal function.

Pathways

Ribosomal RNA processing represents a critical aspect of translation and protein synthesis. RRP7A fits into the ribosome biogenesis pathway ensuring the correct formation and function of ribosomes. This pathway involves several other proteins including FBL (Fibrillarin) and DDX5 which work alongside RRP7A within the SSU processome. Together these components contribute to the intricate steps of rRNA processing from transcription to the assembly and transport of ribosomal subunits. This makes RRP7A integral to maintaining cellular protein synthesis capacity.

Impaired function of RRP7A can be related to ribosomopathies and certain cancer types due to disruptions in ribosome production. In some cancers altered ribosome biogenesis results in increased cellular proliferation. RRP7A disruptions link to proteins such as MYC a well-known oncogene that can promote ribosome biogenesis when overexpressed. Understanding RRP7A's role in these conditions can inform studies exploring the therapeutic potential of targeting ribosome biogenesis pathways in disease treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Nucleolar protein that is involved in ribosomal RNA (rRNA) processing (PubMed : 33199730). Also plays a role in primary cilia resorption, and cell cycle progression in neurogenesis and neocortex development (PubMed : 33199730). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).
See full target information RRP7A
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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