AB168845

Anti-RRP9 antibody

Name: Anti-RRP9 antibody (ab168845) | Abcam
Brand: Abcam Limited
SKU: AB168845
Price: 495 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal RRP9 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human RRP9 aa 150-250.

View Alternative Names

RNU3IP2, U355K, RRP9, U3 small nucleolar RNA-interacting protein 2, RRP9 homolog, U3 small nucleolar ribonucleoprotein-associated 55 kDa protein, U3 snoRNP-associated 55 kDa protein, U3-55K

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Immunoprecipitation - Anti-RRP9 antibody (AB168845)

Detection of RRP9 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168845 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-RRP9 antibody (ab168845)

Predicted band size: 52 kDa

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Western blot - Anti-RRP9 antibody (AB168845)

All lanes:

Western blot - Anti-RRP9 antibody (ab168845) at 0.4 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 52 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human RRP9 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

O43818

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab168845 was affinity purified using an epitope specific to RRP9 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RRP9 also known as Ribosomal RNA Processing 9 is a protein that plays a mechanical role in ribosome biogenesis. It has a molecular mass of approximately 74 kDa. The protein is mainly expressed in the nucleolus the site where ribosomal RNA synthesis and initial steps of ribosome assembly occur. RRP9 contains several conserved motifs that enable it to bind RNA and participate in various processing events essential for this function.

Biological function summary

The protein functions as a component of the small subunit (SSU) processome a large ribonucleoprotein complex. RRP9 contributes to the early pre-rRNA processing steps that produce the small subunit of the ribosome. The SSU processome carries out essential modifications and cleavages necessary for the maturation and functionality of ribosomes. Therefore RRP9 is critical to ensure accurate production of ribosomal subunits.

Pathways

RRP9 is involved in ribosome biogenesis and RNA processing pathways. Through its role in the SSU processome it connects with other proteins such as UTP-C and MPP10 which coordinate these pathways. Ribosome biogenesis is fundamental for protein synthesis and cell proliferation establishing RRP9's importance in these cellular processes. Proper coordination of these pathways is necessary for maintaining cellular homeostasis and function.

RRP9 relates to ribosomopathies conditions caused by defects in ribosomal function. Mutations or dysregulation of RRP9 can result in impaired ribosome assembly contributing to disorders like Diamond-Blackfan anemia. In this disorder RRP9's interaction with other ribosomal proteins like RPS19 becomes disrupted leading to defective erythropoiesis. Additionally abnormal RRP9 expression can be linked to cancer development where increased ribosome production is a common feature.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Component of a nucleolar small nuclear ribonucleoprotein particle (snoRNP) thought to participate in the processing and modification of pre-ribosomal RNA (pre-rRNA) (PubMed : 26867678). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).

See full target information RRP9

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Clinical and translational medicine 13:e1235 PubMed37186134

2023

Therapeutic targeting of CNBP phase separation inhibits ribosome biogenesis and neuroblastoma progression via modulating SWI/SNF complex activity.

Applications

Unspecified application

Species

Unspecified reactive species

Anpei Hu,Guo Chen,Banghe Bao,Yanhua Guo,Dan Li,Xiaojing Wang,Jianqun Wang,Qilan Li,Yi Zhou,Haiyang Gao,Jiyu Song,Xinyi Du,Liduan Zheng,Qiangsong Tong

The Journal of biological chemistry 297:101307 PubMed34662580

2021

Neddylation modification of the U3 snoRNA-binding protein RRP9 by Smurf1 promotes tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Ge Du,Fan Liu,Yan Chang,Shuai Tong,Wei Liu,Yu-Jiao Chen,Ping Xie

Journal of immunology (Baltimore, Md. : 1950) 199:3981-3990 PubMed29070672

2017

Broad Susceptibility of Nucleolar Proteins and Autoantigens to Complement C1 Protease Degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Yitian Cai,Seng Yin Kelly Wee,Junjie Chen,Boon Heng Dennis Teo,Yee Leng Carol Ng,Khai Pang Leong,Jinhua Lu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-RRP9 antibody

Immunoprecipitation - Anti-RRP9 antibody (AB168845)

Western blot - Anti-RRP9 antibody (AB168845)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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