AB188161

Anti-RRS1 antibody

Name: Anti-RRS1 antibody (ab188161) | Abcam
Brand: Abcam Limited
SKU: AB188161

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(7 Publications)

Rabbit Polyclonal RRS1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RRS1 aa 200-300.

View Alternative Names

KIAA0112, RRR, RRS1, Ribosome biogenesis regulatory protein homolog

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRS1 antibody (AB188161)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRS1 antibody (AB188161)

Immunohistochemical analysis of human rectum tissue labeling RRS1 with ab188161 at 1/1000 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRS1 antibody (AB188161)

Immunohistochemical analysis of human fallopian tube tissue labeling RRS1 with ab188161 at 1/1000 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRS1 antibody (AB188161)

Immunohistochemical analysis of human pancreas tissue labeling RRS1 with ab188161 at 1/1000 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRS1 antibody (AB188161)

Immunohistochemical analysis of human cerebral cortex tissue labeling RRS1 with ab188161 at 1/1000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-RRS1 antibody (AB188161)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RRS1 antibody (AB188161)

Immunofluorescence analysis of Human cell line U-2 OS labeling RRS1 with ab188161 at 4 μg/ml.

Supplier Data

Western blot - Anti-RRS1 antibody (AB188161)

All lanes:

Western blot - Anti-RRS1 antibody (ab188161) at 0.4 µg/mL

Lane 1:

PC-3 cell lysate

Lane 2:

HeLa cell lysate

Predicted band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human RRS1 aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

Q15050

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The RRS1 protein also known as Ribosome Biogenesis Regulatory Protein Homolog has a molecular mass of approximately 25 kDa. RRS1 is found primarily in the nucleolus of the cell where it plays an important role in ribosome assembly. It interacts directly with ribosomal RNA (rRNA) facilitating the synthesis and processing of rRNA. The activity of RRS1 is essential for the proper formation of the 60S ribosomal subunit. This protein is widely expressed across various tissue types reflecting its fundamental role in protein synthesis machinery.

Biological function summary

RRS1 interacts as a part of the ribosome biogenesis machinery in eukaryotic cells. It exists within the pre-ribosomal complex partnering with other ribosomal assembly factors to ensure accurate and efficient ribosome formation. The protein is necessary for the transition of the pre-rRNA from the early to late processing stages an essential step for the maturation of functional ribosomes. Through its interactions RRS1 coordinates the assembly and stability of nascent ribosomal subunits linking it integrally with cellular growth and proliferation.

Pathways

RRS1 functions within the ribosome biogenesis pathway pivotal for synthesizing new proteins required for cell function and growth. It completes a vital role in the assembly line of the ribosomal RNA splicing pathway by working with proteins like RPF2 and Syo1. This involvement secures the fidelity of ribosome construction handling critical transitions from pre-ribosomal RNA to mature ribosomes. The well-orchestrated dance of RRS1 and its associates ensure that cellular protein synthesis machinery operates effectively.

RRS1 has been implicated in conditions related to aberrant ribosome biogenesis and function. Dysregulation of RRS1 can contribute to ribosomopathies disorders characterized by defective ribosome production. Disorders like Diamond-Blackfan anemia have links to such dysfunctions as proper ribosomal assembly is compromised. Moreover RRS1's association with proteins like NOP56 in ribosomal biogenesis implies potential impacts on cancer progression where irregular ribosome production might drive abnormal cell proliferation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in ribosomal large subunit assembly. May regulate the localization of the 5S RNP/5S ribonucleoprotein particle to the nucleolus.

See full target information RRS1

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:3265 PubMed38331968

2024

Long noncoding RNA SNHG1 promotes breast cancer progression by regulating the miR-641/RRS1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Deng,Jun Wang,Junying Song,Qinglan Wu,Zunshuang Gong,Jinlian Song,Lin Hou

Molecules (Basel, Switzerland) 28: PubMed37049702

2023

Ribosome Biogenesis Regulator 1 Homolog (RRS1) Promotes Cisplatin Resistance by Regulating AEG-1 Abundance in Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Junying Song,Cuixiu Peng,Runze Wang,Yanan Hua,Qinglan Wu,Lin Deng,Yi Cao,Li Zhang,Lin Hou

Pediatric research : PubMed35523884

2022

RRS1 knockdown inhibits the proliferation of neuroblastoma cell via PI3K/Akt/NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Zhang,Cun Liu,Yi Cao,Li Liu,Fusheng Sun,Lin Hou

International journal of oncology 60: PubMed35179222

2022

Downregulated RRS1 inhibits invasion and metastasis of BT549 through RPL11‑c‑Myc‑SNAIL axis.

Applications

Unspecified application

Species

Unspecified reactive species

Runze Wang,Cuixiu Peng,Junying Song,Yanan Hua,Qinglan Wu,Lin Deng,Yi Cao,Jinyu Zhang,Li Zhang,Li Wu,Lin Hou

Cell cycle (Georgetown, Tex.) 18:2757-2769 PubMed31438772

2019

MicroRNA-598 inhibits the growth and maintenance of gastric cancer stem-like cells by down-regulating RRS1.

Applications

Unspecified application

Species

Unspecified reactive species

Yanling Ma,Fei Yan,Wujie Wei,Jie Deng,Li Li,Li Liu,Jianhai Sun

Oncotarget 8:82968-82980 PubMed29137316

2017

RRS1 silencing suppresses colorectal cancer cell proliferation and tumorigenesis by inhibiting G2/M progression and angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Lin Wu,Zhi-Wen Yang,Li He,Pei-De Dong,Ming-Xing Hou,Xing-Kai Meng,Hai-Ping Zhao,Zhao-Yang Wang,Feng Wang, Baoluri, Wurenqimuge, Agudamu,Yong-Feng Jia,Lin Shi

Oncology reports 38:2166-2172 PubMed28849112

2017

Knockdown of RRS1 by lentiviral-mediated RNAi promotes apoptosis and suppresses proliferation of human hepatocellular carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jitao Wang,Zhi Li,Changzeng Zuo,Qingfan Xie,Hui Li,Junhong Jia,Zhongguang Zhen,Ruizhao Qi,Zhiwei Li,Dengxiang Liu,Baijun Sun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RRS1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRS1 antibody (AB188161)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRS1 antibody (AB188161)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRS1 antibody (AB188161)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRS1 antibody (AB188161)

Immunocytochemistry/ Immunofluorescence - Anti-RRS1 antibody (AB188161)

Western blot - Anti-RRS1 antibody (AB188161)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise