JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB314232

Anti-RS1 antibody [EPR28513-78] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal RS1 antibody. Carrier free. Suitable for ICC/IF, WB, IP, IHC-P, IHC-Fr and reacts with Mouse, Rat, Human samples.

View Alternative Names

XLRS1, RS1, Retinoschisin, X-linked juvenile retinoschisis protein

15 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RS1 antibody [EPR28513-78] - BSA and Azide free (AB314232)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RS1 antibody [EPR28513-78] - BSA and Azide free (AB314232)

This data was developed using ab314231, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human retina tissue labeling RS1 with ab314231 at 1/2000 followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human retina.The section was incubated with ab314231 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RS1 antibody [EPR28513-78] - BSA and Azide free (AB314232)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RS1 antibody [EPR28513-78] - BSA and Azide free (AB314232)

This data was developed using ab314231, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling RS1 with ab314231 at 1/2000 followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on human kidney.The section was incubated with ab314231 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunoprecipitation - Anti-RS1 antibody [EPR28513-78] - BSA and Azide free (AB314232)
  • IP

Supplier Data

Immunoprecipitation - Anti-RS1 antibody [EPR28513-78] - BSA and Azide free (AB314232)

This data was developed using ab314231, the same antibody clone in a different buffer formulation. RS1 was immunoprecipitated from 0.35 mg Human eyeball tissue lysate with ab314231 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314231 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Human eyeball tissue lysate Lane 2 : ab314231 IP in Human eyeball tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab314231 in human eyeball tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 3 seconds

All lanes:

Immunoprecipitation - Anti-RS1 antibody [EPR28513-78] (<a href='/en-us/products/primary-antibodies/rs1-antibody-epr28513-78-ab314231'>ab314231</a>) at 1/30 dilution

All lanes:

Human eyeball tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 3s

Immunohistochemistry (Frozen sections) - Anti-RS1 antibody [EPR28513-78] - BSA and Azide free (AB314232)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-RS1 antibody [EPR28513-78] - BSA and Azide free (AB314232)

This data was developed using ab314231, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat kidney (fresh) tissue labeling RS1 with ab314231 at 1/1000 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Negative control : confocal image showing no staining on rat kidney (PMID : 10915776). The nuclear counterstain was DAPI (Blue). The section was incubated with ab314231 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 dilution.

Immunohistochemistry (Frozen sections) - Anti-RS1 antibody [EPR28513-78] - BSA and Azide free (AB314232)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-RS1 antibody [EPR28513-78] - BSA and Azide free (AB314232)

This data was developed using ab314231, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney (fresh) tissue labeling RS1 with ab314231 at 1/1000 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Negative control : confocal image showing no staining on mouse kidney (PMID : 10915776). The nuclear counterstain was DAPI (Blue). The section was incubated with ab314231 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RS1 antibody [EPR28513-78] - BSA and Azide free (AB314232)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RS1 antibody [EPR28513-78] - BSA and Azide free (AB314232)

This data was developed using ab314231, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse retina tissue labeling RS1 with ab314231 at 1/10000 followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse retina.The section was incubated with ab314231 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 10 mins

Immunohistochemistry (Frozen sections) - Anti-RS1 antibody [EPR28513-78] - BSA and Azide free (AB314232)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-RS1 antibody [EPR28513-78] - BSA and Azide free (AB314232)

This data was developed using ab314231, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse retina (fresh) tissue labeling RS1 with ab314231 at 1/1000 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 11000 dilution (Green). Panel A : merged staining of anti-RS1 (ab314231, green), anti-Map2 (ab225315, red) on mouse retina.Panel B : anti-RS1 stained on mouse retina.Panel C : anti-Map2 stained on mouse retina. The section was incubated in two rounds of staining : in the order of ab314231 and ab225315 for 1 hr at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 dilution.

Immunohistochemistry (Frozen sections) - Anti-RS1 antibody [EPR28513-78] - BSA and Azide free (AB314232)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-RS1 antibody [EPR28513-78] - BSA and Azide free (AB314232)

This data was developed using ab314231, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat retina (fresh) tissue labeling RS1 with ab314231 at 1/1000 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Panel A : merged staining of anti-RS1 (ab314231, green), anti-Map2 (ab225315, red) on rat retina.Panel B : anti-RS1 stained on rat retina.Panel C : anti-Map2 stained on rat retina. The section was incubated in two rounds of staining : in the order of ab314231 and ab225315 for 1 hr at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-RS1 antibody [EPR28513-78] - BSA and Azide free (AB314232)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-RS1 antibody [EPR28513-78] - BSA and Azide free (AB314232)

This data was developed using ab314231, the same antibody clone in a different buffer formulation. Immunofluorescence analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilised Rat primary retina with ab314231 (green) at 1/50 dilution (10.52 µg/ml) followed by ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody at 1/1000 dilution (2 µg/ml). ab11267, Anti-MAP2 mouse monoclonal antibody was used as MAP2 counterstain (red) at 1/500 dilution (4 µg/ml) followed by ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) secondary antibody used at 1/1000 dilution (2 µg/ml). Nuclei were counterstained with DAPI (blue). Confocal image showing cytoplasmic staining in cultured rat primary retina cells. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica - Microsystems, TCS SP8).

Immunocytochemistry/ Immunofluorescence - Anti-RS1 antibody [EPR28513-78] - BSA and Azide free (AB314232)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-RS1 antibody [EPR28513-78] - BSA and Azide free (AB314232)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RS1 antibody [EPR28513-78] - BSA and Azide free (AB314232)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RS1 antibody [EPR28513-78] - BSA and Azide free (AB314232)

This data was developed using ab314231, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat retina tissue labeling RS1 with ab314231 at 1/10000 followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat retina.The section was incubated with ab314231 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentHydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 10 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RS1 antibody [EPR28513-78] - BSA and Azide free (AB314232)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RS1 antibody [EPR28513-78] - BSA and Azide free (AB314232)

This data was developed using ab314231, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling RS1 with ab314231 at 1/10000 followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on rat kidney.The section was incubated with ab314231 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentHydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 10 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RS1 antibody [EPR28513-78] - BSA and Azide free (AB314232)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RS1 antibody [EPR28513-78] - BSA and Azide free (AB314232)

This data was developed using ab314231, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling RS1 with ab314231 at 1/10000 followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on mouse kidney.The section was incubated with ab314231 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentHydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 10 mins

Western blot - Anti-RS1 antibody [EPR28513-78] - BSA and Azide free (AB314232)
  • WB

Supplier Data

Western blot - Anti-RS1 antibody [EPR28513-78] - BSA and Azide free (AB314232)

This data was developed using ab314231, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : kidney, lung(PMID : 9326935). Samples are non-boiled as boiling may cause protein aggregation. The identity of the bands above 37 kDa is unknown. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. Exposure time : Lane 1-3 : 48 seconds, lane 4 : 180 seconds, lane 5-7 6 seconds

All lanes:

Western blot - Anti-RS1 antibody [EPR28513-78] (<a href='/en-us/products/primary-antibodies/rs1-antibody-epr28513-78-ab314231'>ab314231</a>) at 1/1000 dilution

Lane 1:

Human eyeball tissue lysate at 20 µg

Lane 2:

Human kidney tissue lysate at 20 µg

Lane 3:

Human lung tissue lysate at 20 µg

Lane 4:

Mouse eyeball tissue lysate at 20 µg

Lane 5:

Mouse retina tissue lysate at 20 µg

Lane 6:

Mouse kidney tissue lysate at 20 µg

Lane 7:

Mouse lung tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 25 kDa

false

Western blot - Anti-RS1 antibody [EPR28513-78] - BSA and Azide free (AB314232)
  • WB

Supplier Data

Western blot - Anti-RS1 antibody [EPR28513-78] - BSA and Azide free (AB314232)

This data was developed using ab314231, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : lung(PMID : 9326935). Samples are non-boiled as boiling may cause protein aggregation. The identity of the bands above 37 kDa is unknown. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. Exposure time : Lane 1-2 : 180 seconds, lane 3 : 15 seconds

All lanes:

Western blot - Anti-RS1 antibody [EPR28513-78] (<a href='/en-us/products/primary-antibodies/rs1-antibody-epr28513-78-ab314231'>ab314231</a>) at 1/1000 dilution

Lane 1:

Rat eyeball tissue lysate at 20 µg

Lane 2:

Rat lung tissue lysate at 20 µg

Lane 3:

Rat retina tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 25 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28513-78

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

WB, IHC-Fr, IP, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB235974

Recombinant Human RS1 protein (His tag)

SDS-PAGE - Recombinant Human RS1 protein (His tag) (AB235974)

  • 0
  • 0
Primary Antibodies

AB7291

Anti-alpha Tubulin antibody [DM1A] - Loading Control

BOND RX™ Validated

Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (AB7291)

  • 5
  • 92
Primary Antibodies

AB39184

Anti-TIMP3 antibody

Western blot - Anti-TIMP3 antibody (AB39184)

  • 5
  • 5
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
style
left-column

Product details

ab314232 is the carrier-free version of ab314231.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The RS1 protein also known as retinoschisin plays a critical role in cellular adhesion and retinal structure maintenance. It has a molecular mass of approximately 24 kDa. Scientists have observed RS1 expression specifically in the retina suggesting it has a specialized function in retinal health and vision. The protein exists in a dimeric form allowing it to interact efficiently with cellular membranes and extracellular matrices. Its expression is not found in frozen retina samples suggesting changes occur post-mortem or under preservation.
Biological function summary

RS1 is involved in maintaining the structural organization of the retinal layers. It participates in forming a protein complex that helps maintain the extracellular space and regulates cellular adhesion within the retina contributing to the integrity and stability of retinal architecture. The structure of the retina is important for proper signal transduction related to visual processing. The RS1 protein acts as a stabilizing factor reducing retinal detachment and disruptions that can impact vision clarity and focus.

Pathways

Retinoschisin interacts with cellular adhesion pathways and signal transduction within retinal cells. This protein appears to influence the Wnt signaling pathway which plays a role in cell-to-cell communication in the retina. Additionally RS1 associates with the neuropilin protein family which includes proteins involved in axon guidance and vascular patterning therefore influencing the development and maintenance of the retinal structure. These pathways highlight the potential impact of RS1 on visual processing and retinal health.

RS1 mutations have links to X-linked juvenile retinoschisis a retinal disorder leading to vision impairment in males. This disorder manifests as splitting of the retinal layers affecting visual acuity. RS1 interacts with laminin a protein involved in the extracellular matrix and disruptions in this interaction may contribute to disease pathogenesis. Research has also observed RS1's role in retinitis pigmentosa where progressive vision loss occurs and understanding its function provides insights into therapeutic strategies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Binds negatively charged membrane lipids, such as phosphatidylserine and phosphoinositides (By similarity). May play a role in cell-cell adhesion processes in the retina, via homomeric interaction between octamers present on the surface of two neighboring cells (PubMed : 27114531). Required for normal structure and function of the retina (PubMed : 19093009).
See full target information RS1
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com