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AB156568

Anti-rSec6 antibody [EPR10812]

5

(2 Reviews)

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(3 Publications)

Rabbit Recombinant Monoclonal rSec6 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

View Alternative Names

SEC6, SEC6L1, EXOC3, Exocyst complex component 3, Exocyst complex component Sec6

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-rSec6 antibody [EPR10812] (AB156568)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-rSec6 antibody [EPR10812] (AB156568)

Immunofluorescent analysis of HeLa cells labeling rSec6 with ab156568 at 1/100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] (AB156568)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] (AB156568)

Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling rSec6 with ab156568 antibody at 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] (AB156568)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] (AB156568)

Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling rSec6 with ab156568 antibody at 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-rSec6 antibody [EPR10812] (AB156568)
  • IP

Unknown

Immunoprecipitation - Anti-rSec6 antibody [EPR10812] (AB156568)

Immunoprecipitation. ab156568 at 1/1000 staining rSec6 in MCF7 lysate immunoprecipitated using ab156568 at 1/10. Predicted band size : 85 kDa.

All lanes:

Immunoprecipitation - Anti-rSec6 antibody [EPR10812] (ab156568)

Predicted band size: 87 kDa

false

Western blot - Anti-rSec6 antibody [EPR10812] (AB156568)
  • WB

Unknown

Western blot - Anti-rSec6 antibody [EPR10812] (AB156568)

All lanes:

Western blot - Anti-rSec6 antibody [EPR10812] (ab156568) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

MCF7 cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

fetal brain tissue lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 87 kDa

Observed band size: 85 kDa

false

Western blot - Anti-rSec6 antibody [EPR10812] (AB156568)
  • WB

AbReview80461****

Western blot - Anti-rSec6 antibody [EPR10812] (AB156568)

All lanes:

Western blot - Anti-rSec6 antibody [EPR10812] (ab156568) at 1/5000 dilution

Lane 1:

Hela WT at 100000 Cells

Lane 2:

Hela EXOC3 KO at 100000 Cells

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab205718'>ab205718</a>) at 1/5000 dilution

false

This image is courtesy of an anonymous Abreview

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10812

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein rSec6 also known as Sec6 is an integral component of the exocyst complex which plays a role in the targeting and tethering of secretory vesicles to specific sites on the plasma membrane. It has an estimated molecular mass of approximately 97 kDa. rSec6 is expressed across a range of tissue types and is especially noted in actively secreting cells such as those found in neuronal and epithelial tissues.
Biological function summary

RSec6 facilitates the assembly of the exocyst complex which comprises eight proteins and functions in the later stages of the exocytosis pathway. This complex is essential for vesicle fusion and proper secretion processes. By interacting with other exocyst components rSec6 ensures accurate delivery of vesicles containing proteins lipids and other molecules necessary for cell communication and maintenance.

Pathways

RSec6 plays a significant role in both the exocytosis and vesicle transport pathways. The exocyst complex including rSec6 is critical for the vesicle docking and fusion process. rSec6 interacts closely with other exocyst proteins such as Sec5 and Sec8 in these pathways collectively ensuring that vesicles are correctly tethered to areas of active secretion.

RSec6 shows involvement in conditions such as diabetes and some neuronal disorders. Disruption or misregulation of the exocyst complex wherein rSec6 plays a part affects insulin secretion relating to diabetes. Additionally altered rSec6 function can associate with neurodegenerative diseases due to its importance in neuronal cell signaling. The interaction between rSec6 and proteins such as Munc18 and SNAREs is vital in these contexts as these proteins cooperate closely in vesicle fusion processes implicated in several pathophysiological states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.
See full target information EXOC3
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Cell physiology 324:C1249-C1262 PubMed37125772

2023

The exocyst complex is required for the trafficking and delivery of KCa3.1 to the basolateral membrane of polarized epithelia.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew J E Logue,Rachel E Farquhar,Yoakim Eckhoff-Björngard,Tanya T Cheung,Daniel C Devor,Fiona J McDonald,Kirk L Hamilton

The Journal of cell biology 222: PubMed36920342

2023

The exocyst complex is an essential component of the mammalian constitutive secretory pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Conceição Pereira,Danièle Stalder,Georgina S F Anderson,Amber S Shun-Shion,Jack Houghton,Robin Antrobus,Michael A Chapman,Daniel J Fazakerley,David C Gershlick

Cellular signalling 49:1-16 PubMed29729335

2018

Sec6 enhances cell migration and suppresses apoptosis by elevating the phosphorylation of p38 MAPK, MK2, and HSP27.

Applications

WB, IP, ICC/IF

Species

Unspecified reactive species, Unspecified reactive species, Unspecified reactive species

Toshiaki Tanaka,Mitsuyoshi Iino,Kaoru Goto
View all publications
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Product promise

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