Rabbit Recombinant Monoclonal rSec6 antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human samples.
Images
![Immunoprecipitation - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (AB249263), expandable thumbnail](https://content.abcam.com/products/images/rsec6-antibody-epr10812-bsa-and-azide-free-ab249263--immunoprecipitation-img81470.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (AB249263)")
![Western blot - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (AB249263), expandable thumbnail](https://content.abcam.com/products/images/rsec6-antibody-epr10812-bsa-and-azide-free-ab249263--western-blot-img114991.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (AB249263)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (AB249263), expandable thumbnail](https://content.abcam.com/products/images/rsec6-antibody-epr10812-bsa-and-azide-free-ab249263--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111010.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (AB249263)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (AB249263), expandable thumbnail](https://content.abcam.com/products/images/rsec6-antibody-epr10812-bsa-and-azide-free-ab249263--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124157.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (AB249263)")
![Immunocytochemistry/ Immunofluorescence - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (AB249263), expandable thumbnail](https://content.abcam.com/products/images/rsec6-antibody-epr10812-bsa-and-azide-free-ab249263--immunocytochemistry-immunofluorescence-img128793.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (AB249263)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested | Tested |
| Mouse | Predicted | Predicted | Not recommended | Predicted | Predicted |
| Rat | Predicted | Predicted | Not recommended | Predicted | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.
Alternative names
SEC6, SEC6L1, EXOC3, Exocyst complex component 3, Exocyst complex component Sec6
Recommended products
Rabbit Recombinant Monoclonal rSec6 antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR10812
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249263 is the carrier-free version of Anti-rSec6 antibody [EPR10812] ab156568.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein rSec6 also known as Sec6 is an integral component of the exocyst complex which plays a role in the targeting and tethering of secretory vesicles to specific sites on the plasma membrane. It has an estimated molecular mass of approximately 97 kDa. rSec6 is expressed across a range of tissue types and is especially noted in actively secreting cells such as those found in neuronal and epithelial tissues.
- Biological function summary
RSec6 facilitates the assembly of the exocyst complex which comprises eight proteins and functions in the later stages of the exocytosis pathway. This complex is essential for vesicle fusion and proper secretion processes. By interacting with other exocyst components rSec6 ensures accurate delivery of vesicles containing proteins lipids and other molecules necessary for cell communication and maintenance.
- Pathways
RSec6 plays a significant role in both the exocytosis and vesicle transport pathways. The exocyst complex including rSec6 is critical for the vesicle docking and fusion process. rSec6 interacts closely with other exocyst proteins such as Sec5 and Sec8 in these pathways collectively ensuring that vesicles are correctly tethered to areas of active secretion.
- Associated diseases and disorders
RSec6 shows involvement in conditions such as diabetes and some neuronal disorders. Disruption or misregulation of the exocyst complex wherein rSec6 plays a part affects insulin secretion relating to diabetes. Additionally altered rSec6 function can associate with neurodegenerative diseases due to its importance in neuronal cell signaling. The interaction between rSec6 and proteins such as Munc18 and SNAREs is vital in these contexts as these proteins cooperate closely in vesicle fusion processes implicated in several pathophysiological states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunoprecipitation - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (ab249263), expandable thumbnail](https://content.abcam.com/products/images/rsec6-antibody-epr10812-bsa-and-azide-free-ab249263--immunoprecipitation-img81470.jpg "Immunoprecipitation - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (ab249263)")
Immunoprecipitation - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (ab249263)
This data was developed using Anti-rSec6 antibody [EPR10812] ab156568, the same antibody clone in a different buffer formulation.Immunoprecipitation. Anti-rSec6 antibody [EPR10812] ab156568 at 1/1000 staining rSec6 in MCF7 lysate immunoprecipitated using Anti-rSec6 antibody [EPR10812] ab156568 at 1/10. Predicted band size : 85 kDa.
All lanes: Immunoprecipitation - Anti-rSec6 antibody [EPR10812] (Anti-rSec6 antibody [EPR10812] ab156568)
Predicted band size: 87 kDa
![Western blot - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (ab249263), expandable thumbnail](https://content.abcam.com/products/images/rsec6-antibody-epr10812-bsa-and-azide-free-ab249263--western-blot-img114991.jpg "Western blot - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (ab249263)")
Western blot - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (ab249263)
This data was developed using Anti-rSec6 antibody [EPR10812] ab156568, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-rSec6 antibody [EPR10812] (Anti-rSec6 antibody [EPR10812] ab156568) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: MCF7 cell lysate at 10 µg
Lane 3: HepG2 cell lysate at 10 µg
Lane 4: fetal brain tissue lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 87 kDa
Observed band size: 85 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (ab249263), expandable thumbnail](https://content.abcam.com/products/images/rsec6-antibody-epr10812-bsa-and-azide-free-ab249263--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111010.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (ab249263)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (ab249263)
This data was developed using Anti-rSec6 antibody [EPR10812] ab156568, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling rSec6 with Anti-rSec6 antibody [EPR10812] ab156568 antibody at 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (ab249263), expandable thumbnail](https://content.abcam.com/products/images/rsec6-antibody-epr10812-bsa-and-azide-free-ab249263--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124157.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (ab249263)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (ab249263)
This data was developed using Anti-rSec6 antibody [EPR10812] ab156568, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling rSec6 with Anti-rSec6 antibody [EPR10812] ab156568 antibody at 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (ab249263), expandable thumbnail](https://content.abcam.com/products/images/rsec6-antibody-epr10812-bsa-and-azide-free-ab249263--immunocytochemistry-immunofluorescence-img128793.jpg "Immunocytochemistry/ Immunofluorescence - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (ab249263)")
Immunocytochemistry/ Immunofluorescence - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (ab249263)
This data was developed using Anti-rSec6 antibody [EPR10812] ab156568, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labeling rSec6 with Anti-rSec6 antibody [EPR10812] ab156568 at 1/100.
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