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AB249263

Anti-rSec6 antibody [EPR10812] - BSA and Azide free

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Rabbit Recombinant Monoclonal rSec6 antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human samples.

View Alternative Names

SEC6, SEC6L1, EXOC3, Exocyst complex component 3, Exocyst complex component Sec6

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (AB249263)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (AB249263)

This data was developed using ab156568, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labeling rSec6 with ab156568 at 1/100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (AB249263)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (AB249263)

This data was developed using ab156568, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling rSec6 with ab156568 antibody at 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (AB249263)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (AB249263)

This data was developed using ab156568, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling rSec6 with ab156568 antibody at 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (AB249263)
  • IP

Unknown

Immunoprecipitation - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (AB249263)

This data was developed using ab156568, the same antibody clone in a different buffer formulation.Immunoprecipitation. ab156568 at 1/1000 staining rSec6 in MCF7 lysate immunoprecipitated using ab156568 at 1/10. Predicted band size : 85 kDa.

All lanes:

Immunoprecipitation - Anti-rSec6 antibody [EPR10812] (<a href='/en-us/products/primary-antibodies/rsec6-antibody-epr10812-ab156568'>ab156568</a>)

Predicted band size: 87 kDa

false

Western blot - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (AB249263)
  • WB

Unknown

Western blot - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (AB249263)

This data was developed using ab156568, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-rSec6 antibody [EPR10812] (<a href='/en-us/products/primary-antibodies/rsec6-antibody-epr10812-ab156568'>ab156568</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

MCF7 cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

fetal brain tissue lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 87 kDa

Observed band size: 85 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10812

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab249263 is the carrier-free version of ab156568.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein rSec6 also known as Sec6 is an integral component of the exocyst complex which plays a role in the targeting and tethering of secretory vesicles to specific sites on the plasma membrane. It has an estimated molecular mass of approximately 97 kDa. rSec6 is expressed across a range of tissue types and is especially noted in actively secreting cells such as those found in neuronal and epithelial tissues.
Biological function summary

RSec6 facilitates the assembly of the exocyst complex which comprises eight proteins and functions in the later stages of the exocytosis pathway. This complex is essential for vesicle fusion and proper secretion processes. By interacting with other exocyst components rSec6 ensures accurate delivery of vesicles containing proteins lipids and other molecules necessary for cell communication and maintenance.

Pathways

RSec6 plays a significant role in both the exocytosis and vesicle transport pathways. The exocyst complex including rSec6 is critical for the vesicle docking and fusion process. rSec6 interacts closely with other exocyst proteins such as Sec5 and Sec8 in these pathways collectively ensuring that vesicles are correctly tethered to areas of active secretion.

RSec6 shows involvement in conditions such as diabetes and some neuronal disorders. Disruption or misregulation of the exocyst complex wherein rSec6 plays a part affects insulin secretion relating to diabetes. Additionally altered rSec6 function can associate with neurodegenerative diseases due to its importance in neuronal cell signaling. The interaction between rSec6 and proteins such as Munc18 and SNAREs is vital in these contexts as these proteins cooperate closely in vesicle fusion processes implicated in several pathophysiological states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.
See full target information EXOC3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com