Rabbit Recombinant Monoclonal RSF1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 10 publications.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-RSF1 antibody [EPR3749(2)] (AB109002), expandable thumbnail](https://content.abcam.com/products/images/rsf1-antibody-epr37492-ab109002--immunocytochemistry-immunofluorescence-img14603.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-RSF1 antibody [EPR3749(2)] (AB109002)")
![Flow Cytometry (Intracellular) - Anti-RSF1 antibody [EPR3749(2)] (AB109002), expandable thumbnail](https://content.abcam.com/products/images/rsf1-antibody-epr37492-ab109002--flow-cytometry-intracellular-img79992.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-RSF1 antibody [EPR3749(2)] (AB109002)")
![Western blot - Anti-RSF1 antibody [EPR3749(2)] (AB109002), expandable thumbnail](https://content.abcam.com/products/images/rsf1-antibody-epr37492-ab109002--western-blot-img135090.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RSF1 antibody [EPR3749(2)] (AB109002)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] (AB109002), expandable thumbnail](https://content.abcam.com/products/images/rsf1-antibody-epr37492-ab109002--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img43643.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] (AB109002)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] (AB109002), expandable thumbnail](https://content.abcam.com/products/images/rsf1-antibody-epr37492-ab109002--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120568.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] (AB109002)")
Publications
Filter by
- Nucleic acids research 49:e642021aniFOUND: analysing the associated proteome and genomic landscape of the repaired nascent non-replicative chromatin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGeorgios C Stefos,Eszter Szantai,Dimitris Konstantopoulos,Martina Samiotaki,Maria FousteriPubMed 33693861
- Cancer management and research 12:11845-118552020LncRNA NEAT1 Promotes the Progression of Gastric Cancer Through Modifying the miR-1224-5p/RSF1 Signaling Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuoluo Yang et. al.PubMed 33244266
- Cancer biotherapy & radiopharmaceuticals 36:202-2102020lncRNA-PRLB Confers Paclitaxel Resistance of Ovarian Cancer Cells by Regulating RSF1/NF-κB Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuzi Zhao,Li HongPubMed 33156701
- Acta oncologica (Stockholm, Sweden) 59:268-2732019High RSF1 protein expression is an independent prognostic feature in prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDoris Höflmayer,Moslim Hamuda,Cornelia Schroeder,Claudia Hube-Magg,Ronald Simon,Cosima Göbel,Andrea Hinsch,Sören Weidemann,Katharina Möller,Jacob R Izbicki,Frank Jacobsen,Tim Mandelkow,Niclas C Blessin,Florian Lutz,Florian Viehweger,Guido Sauter,Eike Burandt,Patrick Lebok,Maximillian Lennartz,Christoph Fraune,Sarah Minner,Sarah Bonk,Hartwig Huland,Markus Graefen,Thorsten Schlomm,Franziska BüscheckPubMed 31687881
- Nature communications 9:38482018The chromatin remodeler RSF1 controls centromeric histone modifications to coordinate chromosome segregation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHo-Soo Lee et. al.PubMed 30242288
- Oncology letters 16:4279-42842018Effects of RSF-1 on proliferation and apoptosis of breast cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuhui Liu et. al.PubMed 30214561
- Molecules and cells 41:127-1332018Post-Translational Regulation of the RSF1 Chromatin Remodeler under DNA Damage.Applications:WBReactive species:Unspecified reactive speciesSunwoo Min,Yong Won Choi,Hansol Yun,Sujin Jo,Jae-Hoon Ji,Hyeseong ChoPubMed 29385673
- Oncology reports 38:2727-27342017MicroRNA-154 as a prognostic factor in bladder cancer inhibits cellular malignancy by targeting RSF1 and RUNX2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Zhao et. al.PubMed 29048677
- Nature communications 6:79042015The chromatin remodeller RSF1 is essential for PLK1 deposition and function at mitotic kinetochores.
- International journal of clinical and experimental 7:5595-6082014Overexpression of Rsf-1 correlates with pathological type, p53 status and survival in primary breast cancer.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes Antigen retrieval is recommended. For unpurified use at 1/250- 1/500. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes For unpurified use at 1/500-1/1000. |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/80 | Notes - |
Target data
Function
Regulatory subunit of the ATP-dependent RSF-1 and RSF-5 ISWI chromatin-remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair (PubMed:12972596, PubMed:28801535). Binds to core histones together with SMARCA5, and is required for the assembly of regular nucleosome arrays by the RSF-5 ISWI chromatin-remodeling complex (PubMed:12972596). Directly stimulates the ATPase activity of SMARCA1 and SMARCA5 in the RSF-1 and RSF-5 ISWI chromatin-remodeling complexes, respectively (PubMed:28801535). The RSF-1 ISWI chromatin remodeling complex has a lower ATP hydrolysis rate than the RSF-5 ISWI chromatin-remodeling complex (PubMed:28801535). The complexes do not have the ability to slide mononucleosomes to the center of a DNA template (PubMed:28801535). Facilitates transcription of hepatitis B virus (HBV) genes by the pX transcription activator. In case of infection by HBV, together with pX, it represses TNF-alpha induced NF-kappa-B transcription activation. Represses transcription when artificially recruited to chromatin by fusion to a heterogeneous DNA binding domain (PubMed:11788598, PubMed:11944984).
Alternative names
HBXAP, XAP8, RSF1, Remodeling and spacing factor 1, Rsf-1, HBV pX-associated protein 8, Hepatitis B virus X-associated protein, p325 subunit of RSF chromatin-remodeling complex
Recommended products
Rabbit Recombinant Monoclonal RSF1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 10 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR3749(2)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RSF1 also known as Remodeler of Structure of Chromatin 1 is a member of the ATP-dependent chromatin remodeling factors. It has a molecular mass of around 159 kDa. This protein plays a role in altering chromatin architecture by facilitating nucleosome sliding and positioning which is essential for transcriptional regulation. RSF1 expresses in various human tissues particularly in proliferating cells where it assists in regulating gene expression important for cell cycle progression.
- Biological function summary
RSF1 participates in cell division and DNA repair processes functioning as part of the RSF complex. This complex along with other chromatin remodelers alters chromatin structure to allow access to DNA. Such mechanisms are critical for allowing correct transcriptional responses during the cell cycle and in response to DNA damage. By modulating nucleosome positioning RSF1 influences the accessibility of transcription factors and other DNA-binding proteins to chromatin.
- Pathways
RSF1 involves in the transcriptional regulation and DNA repair pathways. It interacts with proteins like SNF2h within the RSF complex. This interaction is necessary to execute its roles effectively in chromatin remodeling. The involvement of RSF1 in signal transduction pathways also links to the regulation of cell proliferation and survival demonstrating its importance in maintaining cellular homeostasis.
- Associated diseases and disorders
RSF1 associates with cancer development and progression including ovarian and liver carcinoma. Overexpression or mutations within the RSF1 gene can lead to oncogenic transformation supporting tumor growth and metastasis. RSF1's association with other proteins such as key regulators in cancer pathways like p53 indicates its significant role in tumor biology. Understanding its function can provide insights into targeted therapies for cancers involving aberrant RSF1 activity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunocytochemistry/ Immunofluorescence - Anti-RSF1 antibody [EPR3749(2)] (ab109002), expandable thumbnail](https://content.abcam.com/products/images/rsf1-antibody-epr37492-ab109002--immunocytochemistry-immunofluorescence-img14603.jpg "Immunocytochemistry/ Immunofluorescence - Anti-RSF1 antibody [EPR3749(2)] (ab109002)")
Immunocytochemistry/ Immunofluorescence - Anti-RSF1 antibody [EPR3749(2)] (ab109002)
Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RSF1 with Purified ab109002 at 1:100 (8.7 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
![Flow Cytometry (Intracellular) - Anti-RSF1 antibody [EPR3749(2)] (ab109002), expandable thumbnail](https://content.abcam.com/products/images/rsf1-antibody-epr37492-ab109002--flow-cytometry-intracellular-img79992.jpg "Flow Cytometry (Intracellular) - Anti-RSF1 antibody [EPR3749(2)] (ab109002)")
Flow Cytometry (Intracellular) - Anti-RSF1 antibody [EPR3749(2)] (ab109002)
Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RSF1 with purified ab109002 at 1/80 dilution (10 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
![Western blot - Anti-RSF1 antibody [EPR3749(2)] (ab109002), expandable thumbnail](https://content.abcam.com/products/images/rsf1-antibody-epr37492-ab109002--western-blot-img135090.jpg "Western blot - Anti-RSF1 antibody [EPR3749(2)] (ab109002)")
Western blot - Anti-RSF1 antibody [EPR3749(2)] (ab109002)
All lanes: Western blot - Anti-RSF1 antibody [EPR3749(2)] (ab109002) at 1/1000 dilution
Lane 1: Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate at 10 µg
Lane 2: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 10 µg
Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg
Predicted band size: 163 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] (ab109002), expandable thumbnail](https://content.abcam.com/products/images/rsf1-antibody-epr37492-ab109002--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img43643.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] (ab109002)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] (ab109002)
Paraffin-embedded human colon tissue stained for RSF1 with ab109002 (unpurified) at a 1/250 dilution in immunohistochemical analysis.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] (ab109002), expandable thumbnail](https://content.abcam.com/products/images/rsf1-antibody-epr37492-ab109002--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120568.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] (ab109002)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] (ab109002)
Paraffin-embedded human endometrial adenocarcinoma tissue stained for RSF1 with ab109002 (unpurified) at a 1/250 dilution in immunohistochemical analysis.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] (ab109002), expandable thumbnail](https://content.abcam.com/products/images/rsf1-antibody-epr37492-ab109002--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img78169.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] (ab109002)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] (ab109002)
Paraffin-embedded human tonsil tissue stained for RSF1 with ab109002 (unpurified) at a 1/250 dilution in immunohistochemical analysis.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] (ab109002), expandable thumbnail](https://content.abcam.com/products/images/rsf1-antibody-epr37492-ab109002--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img78169.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] (ab109002)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com