Rabbit Recombinant Monoclonal RSF1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (AB232564), expandable thumbnail](https://content.abcam.com/products/images/rsf1-antibody-epr37492-bsa-and-azide-free-ab232564--immunocytochemistry-immunofluorescence-img14603.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (AB232564)")
![Flow Cytometry (Intracellular) - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (AB232564), expandable thumbnail](https://content.abcam.com/products/images/rsf1-antibody-epr37492-bsa-and-azide-free-ab232564--flow-cytometry-intracellular-img79992.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (AB232564)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (AB232564), expandable thumbnail](https://content.abcam.com/products/images/rsf1-antibody-epr37492-bsa-and-azide-free-ab232564--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120568.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (AB232564)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (AB232564), expandable thumbnail](https://content.abcam.com/products/images/rsf1-antibody-epr37492-bsa-and-azide-free-ab232564--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img44948.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (AB232564)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (AB232564), expandable thumbnail](https://content.abcam.com/products/images/rsf1-antibody-epr37492-bsa-and-azide-free-ab232564--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img78169.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (AB232564)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Version
1 products for Alternative Product
Target data
Function
Required for assembly of regular nucleosome arrays by the RSF chromatin-remodeling complex (PubMed:12972596). Facilitates transcription of hepatitis B virus (HBV) genes by the pX transcription activator. In case of infection by HBV, together with pX, it represses TNF-alpha induced NF-kappa-B transcription activation. Represses transcription when artificially recruited to chromatin by fusion to a heterogeneous DNA binding domain (PubMed:11944984, PubMed:11788598).
Alternative names
Remodeling and spacing factor 1, Rsf-1, HBV pX-associated protein 8, Hepatitis B virus X-associated protein, p325 subunit of RSF chromatin-remodeling complex, RSF1, HBXAP, XAP8
Recommended products
Rabbit Recombinant Monoclonal RSF1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Alternative names
Remodeling and spacing factor 1, Rsf-1, HBV pX-associated protein 8, Hepatitis B virus X-associated protein, p325 subunit of RSF chromatin-remodeling complex, RSF1, HBXAP, XAP8
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR3749(2)
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab232564 is the carrier-free version of Anti-RSF1 antibody [EPR3749(2)] ab109002.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- Activity summary
RSF1 also known as Remodeler of Structure of Chromatin 1 is a member of the ATP-dependent chromatin remodeling factors. It has a molecular mass of around 159 kDa. This protein plays a role in altering chromatin architecture by facilitating nucleosome sliding and positioning which is essential for transcriptional regulation. RSF1 expresses in various human tissues particularly in proliferating cells where it assists in regulating gene expression important for cell cycle progression.
- Biological function summary
RSF1 participates in cell division and DNA repair processes functioning as part of the RSF complex. This complex along with other chromatin remodelers alters chromatin structure to allow access to DNA. Such mechanisms are critical for allowing correct transcriptional responses during the cell cycle and in response to DNA damage. By modulating nucleosome positioning RSF1 influences the accessibility of transcription factors and other DNA-binding proteins to chromatin.
- Pathways
RSF1 involves in the transcriptional regulation and DNA repair pathways. It interacts with proteins like SNF2h within the RSF complex. This interaction is necessary to execute its roles effectively in chromatin remodeling. The involvement of RSF1 in signal transduction pathways also links to the regulation of cell proliferation and survival demonstrating its importance in maintaining cellular homeostasis.
- Associated diseases and disorders
RSF1 associates with cancer development and progression including ovarian and liver carcinoma. Overexpression or mutations within the RSF1 gene can lead to oncogenic transformation supporting tumor growth and metastasis. RSF1's association with other proteins such as key regulators in cancer pathways like p53 indicates its significant role in tumor biology. Understanding its function can provide insights into targeted therapies for cancers involving aberrant RSF1 activity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunocytochemistry/ Immunofluorescence - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (ab232564), expandable thumbnail](https://content.abcam.com/products/images/rsf1-antibody-epr37492-bsa-and-azide-free-ab232564--immunocytochemistry-immunofluorescence-img14603.jpg "Immunocytochemistry/ Immunofluorescence - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (ab232564)")
Immunocytochemistry/ Immunofluorescence - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (ab232564)
Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RSF1 with Purified Anti-RSF1 antibody [EPR3749(2)] ab109002 at 1:100 (8.7 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RSF1 antibody [EPR3749(2)] ab109002).
![Flow Cytometry (Intracellular) - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (ab232564), expandable thumbnail](https://content.abcam.com/products/images/rsf1-antibody-epr37492-bsa-and-azide-free-ab232564--flow-cytometry-intracellular-img79992.jpg "Flow Cytometry (Intracellular) - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (ab232564)")
Flow Cytometry (Intracellular) - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (ab232564)
Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RSF1 with purified Anti-RSF1 antibody [EPR3749(2)] ab109002 at 1/80 dilution (10 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RSF1 antibody [EPR3749(2)] ab109002).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (ab232564), expandable thumbnail](https://content.abcam.com/products/images/rsf1-antibody-epr37492-bsa-and-azide-free-ab232564--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120568.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (ab232564)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (ab232564)
Paraffin-embedded human endometrial adenocarcinoma tissue stained for RSF1 with Anti-RSF1 antibody [EPR3749(2)] ab109002 (unpurified) at a 1/250 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RSF1 antibody [EPR3749(2)] ab109002).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (ab232564), expandable thumbnail](https://content.abcam.com/products/images/rsf1-antibody-epr37492-bsa-and-azide-free-ab232564--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img44948.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (ab232564)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (ab232564)
Paraffin-embedded human colon tissue stained for RSF1 with Anti-RSF1 antibody [EPR3749(2)] ab109002 (unpurified) at a 1/250 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RSF1 antibody [EPR3749(2)] ab109002).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (ab232564), expandable thumbnail](https://content.abcam.com/products/images/rsf1-antibody-epr37492-bsa-and-azide-free-ab232564--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img78169.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (ab232564)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (ab232564)
Paraffin-embedded human tonsil tissue stained for RSF1 with Anti-RSF1 antibody [EPR3749(2)] ab109002 (unpurified) at a 1/250 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RSF1 antibody [EPR3749(2)] ab109002).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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