AB32526

Anti-RSK1 p90 antibody [Y81]

RabMAb
Recombinant
KO Validated
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(4 Publications)

Rabbit Recombinant Monoclonal RSK1 p90 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

MAPKAPK1A, RSK1, RPS6KA1, Ribosomal protein S6 kinase alpha-1, S6K-alpha-1, 90 kDa ribosomal protein S6 kinase 1, MAP kinase-activated protein kinase 1a, Ribosomal S6 kinase 1, p90-RSK 1, p90RSK1, p90S6K, MAPK-activated protein kinase 1a, MAPKAP kinase 1a, MAPKAPK-1a, RSK-1

3 Images

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Western blot - Anti-RSK1 p90 antibody [Y81] (AB32526)

All lanes:

Western blot - Anti-RSK1 p90 antibody [Y81] (ab32526) at 1/1000 dilution

All lanes:

MCF7 cell lysate

Predicted band size: 82 kDa

Observed band size: 90 kDa

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Lab

Western blot - Anti-RSK1 p90 antibody [Y81] (AB32526)

Western blot : Anti-RSK1 p90 antibody [Y81] ab32526 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 83 kDa in Wild-type MCF7 cell lysates with no signal observed at this size in RPS6KA1 knockout MCF7 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-RSK1 p90 antibody [Y81] (ab32526) at 1/1000 dilution

Lane 1:

Wild-type MCF7 at 20 µg

Lane 2:

Western blot - Human RPS6KA1 knockout MCF7 cell line (ab289311) at 20 µg

Lane 3:

PC-3 at 20 µg

Lane 4:

Calu-3 at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 83 kDa

Observed band size: 83 kDa,37 kDa

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CiteAb

Western blot - Anti-RSK1 p90 antibody [Y81] (AB32526)

RSK1 p90 western blot using anti-RSK1 p90 antibody [Y81] ab32526. Publication image and figure legend from Lu, W., Jia, D., et al., 2017, Sci Rep, PubMed 28827748.

ab32526 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab32526 please see the product overview.

CSEI regulated the expressions of hematopoietic function related proteins in CHRF and K562 cells. After a 24-h CSEI (0, 0.05, 0.1 mg/ml) treatment, the protein expression levels of P-RSK1p90, ELK1 and c-Myc in CHRF cells (a) and K562 cells (b), full-length blots are presented in Supplementary Fig. S5 a, and the expression levels of GATA-1 and GATA-2 in the terminal mature CHRF cells (c) and differentiated K562 cells (d) were detected by western blot, respectively, full-length blots are presented in Supplementary Fig. S5 (b). The quantitative data of these protein levels were normalized by their GAPDH expressions and expressed as a percentage of the corresponding relative intensity of control. The data are shown as the means ± S.E.M. (n = 6). *p < 0.05, **p < 0.01 and ***p < 0.001 versus 0 mg/ml CSEI-treated cells.

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Carrier free

Anti-RSK1 p90 antibody [Y81] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

Y81

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RSK1 also known as p90 ribosomal S6 kinase 1 or p90RSK is a serine/threonine kinase with an approximate mass of 90 kDa. It belongs to the family of proteins that integrate signals from various extracellular stimuli. This kinase is expressed in diverse tissues including brain lung liver and skeletal muscle. At the molecular level RSK1 phosphorylates various target proteins that are involved in regulating cellular growth and survival. Its activity is regulated by phosphorylation events that occur at specific sites such as Serine 238 (E238 in some literature) which are critical for its function.

Biological function summary

RSK1 plays a fundamental role in cell proliferation and differentiation in the context of the p90 protein weight. It is an important component of the mitogen-activated protein kinase (MAPK) signaling pathway and forms part of multi-protein complexes that facilitate its activation and function. RSK1 influences various biological processes by phosphorylating transcription factors histone proteins and other kinases thereby regulating gene expression and protein synthesis. These actions highlight its influence in maintaining cellular homeostasis.

Pathways

RSK1 p90 interacts significantly with the MAPK/ERK and PI3K/AKT pathways. It acts downstream of ERK where it receives activating signals that enable it to phosphorylate substrates within the cell. This role supports its interaction with proteins like Akt and mTOR which are critical mediators of cell growth signals. The interplay between these pathways enables cells to respond to growth factors and stress contributing to the regulation of development and metabolism.

RSK1 is implicated in the progression of cancer and neurological disorders. In cancer abnormal RSK1 signaling can lead to unchecked cell growth and survival where it often interfaces with proteins such as C-RAF and BCL-2 to promote oncogenic pathways. In neurological disorders RSK1 influences pathways that regulate neuron survival and plasticity connecting it to proteins like CREB. Understanding RSK1's role in these conditions can provide insight into targeted therapies that may mitigate these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1 (PubMed : 10679322, PubMed : 12213813, PubMed : 15117958, PubMed : 16223362, PubMed : 17360704, PubMed : 18722121, PubMed : 26158630, PubMed : 35772404, PubMed : 9430688). In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes (PubMed : 18508509, PubMed : 18813292). In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP (PubMed : 12213813, PubMed : 16223362). Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity (PubMed : 18508509, PubMed : 18813292). Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the pre-initiation complex (PubMed : 17360704). In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation (PubMed : 16763566). Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway (PubMed : 15342917). Also involved in feedback regulation of mTORC1 and mTORC2 by phosphorylating DEPTOR (PubMed : 22017876). Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function (PubMed : 10679322, PubMed : 16213824). Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4) (PubMed : 11684016). Mediates induction of hepatocyte prolifration by TGFA through phosphorylation of CEBPB (PubMed : 18508509, PubMed : 18813292). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression (PubMed : 18508509, PubMed : 18813292). Phosphorylates EPHA2 at 'Ser-897', the RPS6KA-EPHA2 signaling pathway controls cell migration (PubMed : 26158630). In response to mTORC1 activation, phosphorylates EIF4B at 'Ser-406' and 'Ser-422' which stimulates bicarbonate cotransporter SLC4A7 mRNA translation, increasing SLC4A7 protein abundance and function (PubMed : 35772404).. (Microbial infection) Promotes the late transcription and translation of viral lytic genes during Kaposi's sarcoma-associated herpesvirus/HHV-8 infection, when constitutively activated.

See full target information RPS6KA1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Aging 13:11150-11169 PubMed33819182

2021

The neuroprotection of deproteinized calf blood extractives injection against Alzheimer's disease via regulation of Nrf-2 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yidi Qu,Wenqi Wang,Tianrui Chen,Yumin Yang,Yizhi Zhang,Di Wang

Cell death & disease 9:578 PubMed29784961

2018

Sarcodon imbricatus polysaccharides improve mouse hematopoietic function after cyclophosphamide-induced damage via G-CSF mediated JAK2/STAT3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Wang,Qiubo Chu,Xue Jiang,Yue Yu,Libian Wang,Yaqi Cui,Jiahui Lu,Lirong Teng,Di Wang

PLoS pathogens 13:e1006580 PubMed28957431

2017

Male hormones activate EphA2 to facilitate Kaposi's sarcoma-associated herpesvirus infection: Implications for gender disparity in Kaposi's sarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Wang,Zhe Zou,Zhaohui Deng,Deguang Liang,Xin Zhou,Rui Sun,Ke Lan

Scientific reports 7:8402 PubMed28827748

2017

Calf Spleen Extractive Injection protects mice against cyclophosphamide-induced hematopoietic injury through G-CSF-mediated JAK2/STAT3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Wenqian Lu,Dongxu Jia,Shengshu An,Ming Mu,Xinan Qiao,Yan Liu,Xin Li,Di Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RSK1 p90 antibody [Y81]

Western blot - Anti-RSK1 p90 antibody [Y81] (AB32526)

Western blot - Anti-RSK1 p90 antibody [Y81] (AB32526)

Western blot - Anti-RSK1 p90 antibody [Y81] (AB32526)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise