AB326649

Anti-RSK1 p90 (phospho S308) antibody [SR2279]

Recombinant
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Rabbit Recombinant Monoclonal RSK1 p90 phospho S380 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples.

View Alternative Names

MAPKAPK1A, RSK1, RPS6KA1, Ribosomal protein S6 kinase alpha-1, S6K-alpha-1, 90 kDa ribosomal protein S6 kinase 1, MAP kinase-activated protein kinase 1a, Ribosomal S6 kinase 1, p90-RSK 1, p90RSK1, p90S6K, MAPK-activated protein kinase 1a, MAPKAP kinase 1a, MAPKAPK-1a, RSK-1

4 Images

Supplier Data

Western blot - Anti-RSK1 p90 (phospho S308) antibody [SR2279] (AB326649)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : Hela whole cell lysates
Lane 2 : Hela treated with 200nM PMA for 30min whole cell lysates
Lane 3 : Hela treated with 100ng/ml EGF for 20min whole cell lysates
Lane 4 : JK whole cell lysates
Lane 5 : JK treated with 200nM PMA for 30min whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size : 83 kDa
Observed band size : 83 kDa

Exposure time : 6 seconds

All lanes:

Western blot - Anti-RSK1 p90 (phospho S308) antibody [SR2279] (ab326649) at 1/1000 dilution

Lane 1:

Hela whole cell lysates at 20 µg

Lane 2:

Hela treated with 200nM PMA for 30min whole cell lysates at 20 µg

Lane 3:

Hela treated with 100ng/ml EGF for 20min whole cell lysates at 20 µg

Lane 4:

JK whole cell lysates at 20 µg

Lane 5:

JK treated with 200nM PMA for 30min whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 83 kDa

Observed band size: 83 kDa

false

Exposure time: 6s

Supplier Data

Western blot - Anti-RSK1 p90 (phospho S308) antibody [SR2279] (AB326649)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : A549 whole cell lysates
Lane 2 : A431 whole cell lysates
Lane 3 : JK whole cell lysates
Lane 4 : 3T3 whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size : 83 kDa
Observed band size : 83 kDa

Exposure time : 5 seconds

All lanes:

Western blot - Anti-RSK1 p90 (phospho S308) antibody [SR2279] (ab326649) at 1/1000 dilution

Lane 1:

A549 whole cell lysates at 20 µg

Lane 2:

A431 whole cell lysates at 20 µg

Lane 3:

JK whole cell lysates at 20 µg

Lane 4:

3T3 whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 83 kDa

Observed band size: 83 kDa

false

Exposure time: 5s

Supplier Data

Western blot - Anti-RSK1 p90 (phospho S308) antibody [SR2279] (AB326649)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 : Phospho-p90 S6K alpha 1 (S380) knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.

All lanes:

Western blot - Anti-RSK1 p90 (phospho S308) antibody [SR2279] (ab326649) at 1/1000 dilution

Lane 1:

Wild-type Hela cell lysate at 20 µg

Lane 2:

RSK1 p90 (phospho S308) knockdown Hela cell lysate at 20 µg

false

Supplier Data

Western blot - Anti-RSK1 p90 (phospho S308) antibody [SR2279] (AB326649)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : Mouse Lung lysates
Lane 2 : Rat Skin lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size : 83 kDa
Observed band size : 83 kDa

Exposure time : 5 seconds

All lanes:

Western blot - Anti-RSK1 p90 (phospho S308) antibody [SR2279] (ab326649) at 1/1000 dilution

Lane 1:

Mouse Lung lysates at 20 µg

Lane 2:

Rat Skin lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 83 kDa

Observed band size: 83 kDa

false

Exposure time: 5s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SR2279

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1 (PubMed : 10679322, PubMed : 12213813, PubMed : 15117958, PubMed : 16223362, PubMed : 17360704, PubMed : 18722121, PubMed : 26158630, PubMed : 35772404, PubMed : 9430688). In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes (PubMed : 18508509, PubMed : 18813292). In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP (PubMed : 12213813, PubMed : 16223362). Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity (PubMed : 18508509, PubMed : 18813292). Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the pre-initiation complex (PubMed : 17360704). In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation (PubMed : 16763566). Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway (PubMed : 15342917). Also involved in feedback regulation of mTORC1 and mTORC2 by phosphorylating DEPTOR (PubMed : 22017876). Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function (PubMed : 10679322, PubMed : 16213824). Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4) (PubMed : 11684016). Mediates induction of hepatocyte prolifration by TGFA through phosphorylation of CEBPB (PubMed : 18508509, PubMed : 18813292). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression (PubMed : 18508509, PubMed : 18813292). Phosphorylates EPHA2 at 'Ser-897', the RPS6KA-EPHA2 signaling pathway controls cell migration (PubMed : 26158630). In response to mTORC1 activation, phosphorylates EIF4B at 'Ser-406' and 'Ser-422' which stimulates bicarbonate cotransporter SLC4A7 mRNA translation, increasing SLC4A7 protein abundance and function (PubMed : 35772404).. (Microbial infection) Promotes the late transcription and translation of viral lytic genes during Kaposi's sarcoma-associated herpesvirus/HHV-8 infection, when constitutively activated.

See full target information RPS6KA1 pS380

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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