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Rabbit Recombinant Monoclonal RSK1 p90 phospho S380 antibody. Carrier free. Suitable for Dot, ICC/IF, IP, WB and reacts with Synthetic peptide, Human, Rat, Mouse samples.

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Images

Immunoprecipitation - Anti-RSK1 p90 (phospho S380) antibody [E239] - BSA and Azide free (AB247241), expandable thumbnail
  • Western blot - Anti-RSK1 p90 (phospho S380) antibody [E239] - BSA and Azide free (AB247241), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-RSK1 p90 (phospho S380) antibody [E239] - BSA and Azide free (AB247241), expandable thumbnail
  • Dot Blot - Anti-RSK1 p90 (phospho S380) antibody [E239] - BSA and Azide free (AB247241), expandable thumbnail
  • Western blot - Anti-RSK1 p90 (phospho S380) antibody [E239] - BSA and Azide free (AB247241), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotICC/IFIPWB
Human
Expected
Tested
Expected
Tested
Mouse
Expected
Expected
Expected
Tested
Rat
Expected
Expected
Tested
Tested
Synthetic peptide
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Synthetic peptide
Dilution info
-
Notes

-

Expected
Expected

Species
Human, Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
-
Notes

-

Expected
Expected

Species
Human, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Target data

Function

Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1 (PubMed:10679322, PubMed:12213813, PubMed:15117958, PubMed:16223362, PubMed:17360704, PubMed:18722121, PubMed:26158630, PubMed:35772404, PubMed:9430688). In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes (PubMed:18508509, PubMed:18813292). In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP (PubMed:12213813, PubMed:16223362). Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity (PubMed:18508509, PubMed:18813292). Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the pre-initiation complex (PubMed:17360704). In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation (PubMed:16763566). Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway (PubMed:15342917). Also involved in feedback regulation of mTORC1 and mTORC2 by phosphorylating DEPTOR (PubMed:22017876). Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function (PubMed:10679322, PubMed:16213824). Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4) (PubMed:11684016). Mediates induction of hepatocyte prolifration by TGFA through phosphorylation of CEBPB (PubMed:18508509, PubMed:18813292). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression (PubMed:18508509, PubMed:18813292). Phosphorylates EPHA2 at 'Ser-897', the RPS6KA-EPHA2 signaling pathway controls cell migration (PubMed:26158630). In response to mTORC1 activation, phosphorylates EIF4B at 'Ser-406' and 'Ser-422' which stimulates bicarbonate cotransporter SLC4A7 mRNA translation, increasing SLC4A7 protein abundance and function (PubMed:35772404). (Microbial infection) Promotes the late transcription and translation of viral lytic genes during Kaposi's sarcoma-associated herpesvirus/HHV-8 infection, when constitutively activated.

Alternative names

MAPKAPK1A, RSK1, RPS6KA1, Ribosomal protein S6 kinase alpha-1, S6K-alpha-1, 90 kDa ribosomal protein S6 kinase 1, MAP kinase-activated protein kinase 1a, Ribosomal S6 kinase 1, p90-RSK 1, p90RSK1, p90S6K, MAPK-activated protein kinase 1a, MAPKAP kinase 1a, MAPKAPK-1a, RSK-1

Recommended products

Rabbit Recombinant Monoclonal RSK1 p90 phospho S380 antibody. Carrier free. Suitable for Dot, ICC/IF, IP, WB and reacts with Synthetic peptide, Human, Rat, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
E239
Purification technique
Affinity purification Protein A
Specificity

The antibody only detects RSK1 phosphorylated on Serine 380.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab247241 is the carrier-free version of Anti-RSK1 p90 (phospho S380) antibody [E239] ab32203.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

RSK1 also known as p90 ribosomal S6 kinase 1 or p90RSK is a serine/threonine kinase with an approximate mass of 90 kDa. It belongs to the family of proteins that integrate signals from various extracellular stimuli. This kinase is expressed in diverse tissues including brain lung liver and skeletal muscle. At the molecular level RSK1 phosphorylates various target proteins that are involved in regulating cellular growth and survival. Its activity is regulated by phosphorylation events that occur at specific sites such as Serine 238 (E238 in some literature) which are critical for its function.

Biological function summary

RSK1 plays a fundamental role in cell proliferation and differentiation in the context of the p90 protein weight. It is an important component of the mitogen-activated protein kinase (MAPK) signaling pathway and forms part of multi-protein complexes that facilitate its activation and function. RSK1 influences various biological processes by phosphorylating transcription factors histone proteins and other kinases thereby regulating gene expression and protein synthesis. These actions highlight its influence in maintaining cellular homeostasis.

Pathways

RSK1 p90 interacts significantly with the MAPK/ERK and PI3K/AKT pathways. It acts downstream of ERK where it receives activating signals that enable it to phosphorylate substrates within the cell. This role supports its interaction with proteins like Akt and mTOR which are critical mediators of cell growth signals. The interplay between these pathways enables cells to respond to growth factors and stress contributing to the regulation of development and metabolism.

Associated diseases and disorders

RSK1 is implicated in the progression of cancer and neurological disorders. In cancer abnormal RSK1 signaling can lead to unchecked cell growth and survival where it often interfaces with proteins such as C-RAF and BCL-2 to promote oncogenic pathways. In neurological disorders RSK1 influences pathways that regulate neuron survival and plasticity connecting it to proteins like CREB. Understanding RSK1's role in these conditions can provide insight into targeted therapies that may mitigate these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunoprecipitation - Anti-RSK1 p90 (phospho S380) antibody [E239] - BSA and Azide free (ab247241), expandable thumbnail

    Immunoprecipitation - Anti-RSK1 p90 (phospho S380) antibody [E239] - BSA and Azide free (ab247241)

    This data was developed using Anti-RSK1 p90 (phospho S380) antibody [E239] ab32203, the same antibody clone in a different buffer formulation.
    Purified Anti-RSK1 p90 (phospho S380) antibody [E239] ab32203 at 1:20 dilution (0.8μg) immunoprecipitating RSK1 p90 in C6 whole cell lysate treated with 100nM Calyculin A for 30 minutes.


    Lane 1 (input): C6 (Rat glial tumor glial cell) whole cell lysate treated with 100nM Calyculin A for 30 minutes 10μg.
    Lane 2 (+): Anti-RSK1 p90 (phospho S380) antibody [E239] ab32203 + C6 whole cell lysate treated with 100nM Calyculin A for 30 minutes.
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-RSK1 p90 (phospho S380) antibody [E239] ab32203 in C6 whole cell lysate treated with 100nM Calyculin A for 30 minutes.
    VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) (1:1000 dilution) was used for Western blotting.


    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 90 kDa

    All lanes: Immunoprecipitation - Anti-RSK1 p90 (phospho S380) antibody [E239] (Anti-RSK1 p90 (phospho S380) antibody [E239] ab32203)

    Predicted band size: 82 kDa

  • Western blot - Anti-RSK1 p90 (phospho S380) antibody [E239] - BSA and Azide free (ab247241), expandable thumbnail

    Western blot - Anti-RSK1 p90 (phospho S380) antibody [E239] - BSA and Azide free (ab247241)

    This data was developed using Anti-RSK1 p90 (phospho S380) antibody [E239] ab32203, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Image produced using the purified version.

    All lanes: Western blot - Anti-RSK1 p90 (phospho S380) antibody [E239] (Anti-RSK1 p90 (phospho S380) antibody [E239] ab32203) at 1/1000 dilution

    Lane 1: Untreated PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate at 15 µg

    Lane 2: PC-12 (Rat adrenal gland pheochromocytoma ) treated with 200ng/ml NGF for 24 hours whole cell lysate at 15 µg

    Lane 3: PC-12 (Rat adrenal gland pheochromocytoma ) treated with 200ng/ml NGF for 24 hours whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 82 kDa

    Observed band size: 90 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RSK1 p90 (phospho S380) antibody [E239] - BSA and Azide free (ab247241), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RSK1 p90 (phospho S380) antibody [E239] - BSA and Azide free (ab247241)

    This data was developed using Anti-RSK1 p90 (phospho S380) antibody [E239] ab32203, the same antibody clone in a different buffer formulation.
    Immunocytochemistry analysis of A431 (Human epidermoid carcinoma epithelial cell) treated with 100ng/ml EGF for 10 minutes cells labeling RSK1 p90 with Purified Anti-RSK1 p90 (phospho S380) antibody [E239] ab32203 at 1:50 dilution (2.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Dot Blot - Anti-RSK1 p90 (phospho S380) antibody [E239] - BSA and Azide free (ab247241), expandable thumbnail

    Dot Blot - Anti-RSK1 p90 (phospho S380) antibody [E239] - BSA and Azide free (ab247241)

    This data was developed using Anti-RSK1 p90 (phospho S380) antibody [E239] ab32203, the same antibody clone in a different buffer formulation.

    This data was produced using the unpurified format.

    Dot blot analysis of RSK1 p90 (phospho S380) phospho peptide (Lane 1) and RSK1 p90 non-phospho peptide (Lane 2) labeling RSK1 p90 (phospho S380) with Anti-RSK1 p90 (phospho S380) antibody [E239] ab32203 at a dilution of 1/1000. Goat Anti-Rabbit IgG H&L (HRP) ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/100 000 was used as the secondary antibody. Blocking and diluting buffer: 5% NFDM/TBST. Exposure time: 3 minutes.

  • Western blot - Anti-RSK1 p90 (phospho S380) antibody [E239] - BSA and Azide free (ab247241), expandable thumbnail

    Western blot - Anti-RSK1 p90 (phospho S380) antibody [E239] - BSA and Azide free (ab247241)

    This data was developed using Anti-RSK1 p90 (phospho S380) antibody [E239] ab32203, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Image produced using the purified version.

    All lanes: Western blot - Anti-RSK1 p90 (phospho S380) antibody [E239] (Anti-RSK1 p90 (phospho S380) antibody [E239] ab32203) at 1/1000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight whole cell lysate at 15 µg

    Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight then treated with 200nM TPA for 4 hours whole cell lysate at 15 µg

    Lane 3: HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight then treated with 200nM TPA for 4 hours whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 82 kDa

    Observed band size: 90 kDa

  • Western blot - Anti-RSK1 p90 (phospho S380) antibody [E239] - BSA and Azide free (ab247241), expandable thumbnail

    Western blot - Anti-RSK1 p90 (phospho S380) antibody [E239] - BSA and Azide free (ab247241)

    This data was developed using Anti-RSK1 p90 (phospho S380) antibody [E239] ab32203, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Image produced using the purified version.

    All lanes: Western blot - Anti-RSK1 p90 (phospho S380) antibody [E239] (Anti-RSK1 p90 (phospho S380) antibody [E239] ab32203) at 1/1000 dilution

    Lane 1: NIH/3T3 (Mouse embryonic fibroblast) starved for 24 hours whole cell lysate at 15 µg

    Lane 2: NIH/3T3 (Mouse embryonic fibroblast) starved for 24 hours then treated with 100nM Calyculin A for 30 minutes whole cell lysate at 15 µg

    Lane 3: NIH/3T3 (Mouse embryonic fibroblast) starved for 24 hours then treated with 100nM Calyculin A for 30 minutes whole cell lysate, and then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 82 kDa

    Observed band size: 90 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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