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Rabbit Polyclonal RSK3 antibody. Suitable for WB and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Ribosomal protein S6 kinase alpha-2.

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Images

Western blot - Anti-RSK3 antibody (AB227230), expandable thumbnail
  • Western blot - Anti-RSK3 antibody (AB227230), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human Ribosomal protein S6 kinase alpha-2. The exact immunogen used to generate this antibody is proprietary information. Database link Q15349

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Predicted
Cow
Predicted

Tested
Tested

Species

Mouse

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Predicted
Predicted

Species

Rat, Cow

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of transcription factors, regulates translation, and mediates cellular proliferation, survival, and differentiation. May function as tumor suppressor in epithelial ovarian cancer cells.

Alternative names

Recommended products

Rabbit Polyclonal RSK3 antibody. Suitable for WB and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Ribosomal protein S6 kinase alpha-2.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human Ribosomal protein S6 kinase alpha-2. The exact immunogen used to generate this antibody is proprietary information. Database link Q15349
Purification technique

Affinity purification Immunogen

Specificity

Based on sequence alignment of the used immunogen, this antibody may cross-react with RSK2. However, we have no experimental testing data for cross-reactivity of ab227230 with other RSK proteins.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

RSK3 also known as ribosomal S6 kinase 2 or RPS6KA2 is a serine/threonine-protein kinase with a molecular mass of about 90 kDa. RSK3 belongs to the p90-RSK family of kinases comprising four isoforms (RSK1 RSK2 RSK3 and RSK4) which are downstream effectors of the MAPK/ERK pathway. It is ubiquitously expressed with significant expression in the brain heart and skeletal muscle.

Biological function summary

RSK3 acts in cell growth survival and differentiation. The kinase participates in forming signaling complexes that transmit signals from the cell membrane to the nucleus. RSK3 modulates the activity of several substrates including transcription factors and other kinases by phosphorylating specific serine/threonine residues. RSK3 interacts with complexes involved in cell adhesion and migration facilitating cellular responses to external stimuli.

Pathways

RSK3 plays a role in the MAPK/ERK pathway which is important for mediating responses to growth factors. This pathway interlinks with other signaling cascades including those involving the PI3K/AKT pathway. RSK3 phosphorylates substrates such as ribosomal protein S6 and the transcription factor CREB which influence protein synthesis and gene expression. Its related proteins such as RSK1 and RSK2 also contribute to fine-tuning these cellular pathways.

Associated diseases and disorders

RSK3 is associated with cancer and cardiac hypertrophy. Dysregulation of RSK3 signaling can lead to enhanced cell proliferation and reduced apoptosis contributing to tumorigenesis. It interacts with other proteins including AKT in pathways that promote cancer cell survival. In cardiac hypertrophy RSK3 plays a role in maladaptive processes leading to heart enlargement linked to interaction with proteins like ERK. Understanding RSK3's role in these conditions can guide therapeutic strategies targeting its signaling pathways.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-RSK3 antibody (ab227230), expandable thumbnail

    Western blot - Anti-RSK3 antibody (ab227230)

    7.5% SDS-PAGE gel.

    All lanes: Western blot - Anti-RSK3 antibody (ab227230) at 1/1000 dilution

    All lanes: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

    Predicted band size: 83 kDa

  • Western blot - Anti-RSK3 antibody (ab227230), expandable thumbnail

    Western blot - Anti-RSK3 antibody (ab227230)

    7.5% SDS-PAGE gel.

    All lanes: Western blot - Anti-RSK3 antibody (ab227230) at 1/1000 dilution

    All lanes: Mouse brain lysate at 50 µg

    Predicted band size: 83 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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