Anti-RSK4 antibody [EP1982Y] (ab76117)

Rabbit Recombinant Monoclonal RSK4 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 7 publications.

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Images

Western blot - Anti-RSK4 antibody [EP1982Y] (AB76117), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	WB	ICC/IF	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Expected
Mouse	Tested	Tested	Expected	Tested
Rat	Tested	Expected	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/50 - 1/250	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species Rat	Dilution info 1/50 - 1/250	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species Human	Dilution info 1/50 - 1/250	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/250 - 1/1000	Notes -
Species Human	Dilution info 1/250 - 1/1000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100 - 1/1000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/25 - 1/70	Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species	Dilution info	Notes
Species Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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Target data

See full target information RPS6KA6

Function

Constitutively active serine/threonine-protein kinase that exhibits growth-factor-independent kinase activity and that may participate in p53/TP53-dependent cell growth arrest signaling and play an inhibitory role during embryogenesis.

Alternative names

RSK4, RPS6KA6, Ribosomal protein S6 kinase alpha-6, S6K-alpha-6, 90 kDa ribosomal protein S6 kinase 6, Ribosomal S6 kinase 4, pp90RSK4, p90-RSK 6, p90RSK6, RSK-4

Recommended products

Rabbit Recombinant Monoclonal RSK4 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 7 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EP1982Y
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

RSK4 also known as Ribosomal S6 Kinase 4 or RPS6KA6 is a protein that functions as a serine/threonine kinase. It plays a significant role in intracellular signaling. RSK4 has a molecular mass of approximately 80 kDa. It belongs to the RSK family of proteins which share a characteristic kinase domain structure. Expression of RSK4 is seen in various tissues including brain kidney and endothelial cells indicating its diverse functional roles across different cell types and systems.

Biological function summary

RSK4 has a role in regulating cell growth and survival. It can influence transcriptional processes and protein synthesis. Despite being not extensively documented in biological research as other family members its kinase activity suggests that it participates in various cellular processes by phosphorylating specific substrates. RSK4 operates as an individual kinase while its related family members may form complexes for more coordinated activities. Its action in cell cycle regulation highlights its importance in maintaining cellular function.

Pathways

RSK4 integrates into well-known signaling pathways including the MAPK/ERK pathway. This association implies it has a role in the regulation of gene expression mitosis and cell survival linking it with other kinases like ERK1 and ERK2. Furthermore it can interact with pathways involving the PI3K/AKT signaling cascade contributing to additional regulation of cell growth thereby influencing diverse physiological functions. The involvement of RSK4 in these pathways highlights its potential interactions with other proteins such as MEK and mTOR which are pivotal in driving intracellular responses.

Associated diseases and disorders

RSK4 has associations with various conditions including cancer and specific genetic disorders. It has been linked with tumor suppression suggesting that dysregulation of RSK4 may contribute to oncogenic processes. Additionally alterations in RSK4 expression or activity have potential connections with mental health disorders due to its expression in the brain. It interacts biologically with proteins like p53 and other growth factor receptors which are also implicated in these disease processes. Understanding RSK4's role in these contexts can provide insights into therapeutic targets for managing such conditions.

Product promise

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10 product images

Western blot - Anti-RSK4 antibody [EP1982Y] (ab76117)
Lane 1: Wild-type HAP1 cell lysate (40 μg)
Lane 2: RSK4 knockout HAP1 cell lysate (40 μg)
Lane 3: MCF7 cell lysate (20 μg)
Lane 4: HEK293 cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab76117 observed at 90 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
ab76117 was shown to recognize RSK4 when RSK4 knockout samples were used, along with additional cross-reactive bands. Wild-type and RSK4 knockout samples were subjected to SDS-PAGE. ab76117 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted at 1/250 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-RSK4 antibody [EP1982Y] (ab76117)
Predicted band size: 39 kDa, 84 kDa
Western blot - Anti-RSK4 antibody [EP1982Y] (ab76117)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-RSK4 antibody [EP1982Y] (ab76117) at 1/1000 dilution
Lane 1: SH-SY5Y cell lysate at 20 µg
Lane 2: Caco-2 cell lysate at 20 µg
Secondary
All lanes: HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 84 kDa
Observed band size: 90 kDa
Immunocytochemistry/ Immunofluorescence - Anti-RSK4 antibody [EP1982Y] (ab76117)
Immunofluorescence staining of HEK293 cells with purified ab76117 at a working dilution of 1/1000, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti-rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), used at a dilution of 1/1000. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (Alexa Fluor^® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab76117 was used at a dilution of 1/500 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at a dilution of 1/500. For negative control 2, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor^® 488 goat anti-rabbit antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at a dilution of 1/400.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSK4 antibody [EP1982Y] (ab76117)
Immunohistochemical staining of paraffin embedded rat kidney with purified ab76117 at a working dilution of 1/250. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSK4 antibody [EP1982Y] (ab76117)
Immunohistochemical staining of paraffin embedded mouse cerebral cortex with purified ab76117 at a working dilution of 1/250. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSK4 antibody [EP1982Y] (ab76117)
Immunohistochemical staining of paraffin embedded human kidney with purified ab76117 at a working dilution of 1/250. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Flow Cytometry (Intracellular) - Anti-RSK4 antibody [EP1982Y] (ab76117)
Overlay histogram showing Neuro-2a cells fixed in 2% PFA and stained with purified ab76117 at a dilution of 1 in 70 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).
Western blot - Anti-RSK4 antibody [EP1982Y] (ab76117)
All lanes: Western blot - Anti-RSK4 antibody [EP1982Y] (ab76117) at 1/500 dilution
All lanes: SH-SY5Y cell lysate at 10 µg
Secondary
All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 84 kDa
Observed band size: 90 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSK4 antibody [EP1982Y] (ab76117)
Unpurified ab76117, at 1/50 dilution, staining RSK4 in paraffin-embedded human brain glioma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-RSK4 antibody [EP1982Y] (ab76117)
Immunofluorescent staining of SH-Sy5y cells using unpurified ab76117 at 1/100 dilution.