AB284772

Anti-RSK4 (phospho S232) antibody [EP1524Y] - BSA and Azide free

RabMAb
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Rabbit Monoclonal RSK4 phospho S232 antibody. Carrier free. Suitable for ELISA, Dot, WB, IHC-P and reacts with Synthetic peptide, Human samples.

View Alternative Names

RSK4, RPS6KA6, Ribosomal protein S6 kinase alpha-6, S6K-alpha-6, 90 kDa ribosomal protein S6 kinase 6, Ribosomal S6 kinase 4, pp90RSK4, p90-RSK 6, p90RSK6, RSK-4

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSK4 (phospho S232) antibody [EP1524Y] - BSA and Azide free (AB284772)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSK4 (phospho S232) antibody [EP1524Y] - BSA and Azide free (AB284772)

This data was developed using ab81290, the same antibody clone in a different buffer formulation.ab81290, at 1/50, staining human muscle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-RSK4 (phospho S232) antibody [EP1524Y] - BSA and Azide free (AB284772)

This data was developed using ab81290, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RSK4 (phospho S232) antibody [EP1524Y] (<a href='/en-us/products/primary-antibodies/rsk4-phospho-s232-antibody-ep1524y-ab81290'>ab81290</a>) at 1/10000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate untreated at 10 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate treated with Calyculin A at 10 µg

Secondary

All lanes:

HRP labelled anti-rabbit at 1/2000 dilution

Predicted band size: 84 kDa

Observed band size: 84 kDa

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ELISA - Anti-RSK4 (phospho S232) antibody [EP1524Y] - BSA and Azide free (AB284772)

ELISA

Supplier Data

ELISA - Anti-RSK4 (phospho S232) antibody [EP1524Y] - BSA and Azide free (AB284772)

This data was developed using ab81290, the same antibody clone in a different buffer formulation.Direct ELISA antigen dose-response curve using ab81290.

Primary antibody : ab81290 (0-1000 ng/ml).

Antigens :

RSK3 (phospho S218) phopspho peptide

RSK3 non-phopspho peptide

RSK4 (phospho S232) phopspho peptide

RSK4 non-phopspho peptide

BSA-branched PEG 40k (0-100 ng/ml).

Secondary antibody : Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at 1/2500 dilution.

Supplier Data

Dot Blot - Anti-RSK4 (phospho S232) antibody [EP1524Y] - BSA and Azide free (AB284772)

This data was developed using ab81290, the same antibody clone in a different buffer formulation.Dot blot analysis of RSK4 (pS232) phospho peptide (Lane 1), RSK4 non-phospho peptide (Lane 2), RSK3 (pS218) phospho peptide (Lane 3) and RSK3 non-phospho peptide (Lane 4) labeling RSK4 (phospho S232) with ab81290 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/100000.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

Unconjugated

Anti-RSK4 (phospho S232) antibody [EP1524Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1524Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, ELISA, WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Due to high sequence homology, this antibody might cross-react with RSK1(Q15418) and RSK3(P51812).

This antibody cross-reactivates with PSK3 (phospho S218). The immunogen used for this product shares 100% homology with RSK1 and RSK2, Cross-reactivity with these two proteins has not been confirmed experimentally.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab284772 is the carrier-free version of ab81290

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RSK4 also known as Ribosomal S6 Kinase 4 or RPS6KA6 is a protein that functions as a serine/threonine kinase. It plays a significant role in intracellular signaling. RSK4 has a molecular mass of approximately 80 kDa. It belongs to the RSK family of proteins which share a characteristic kinase domain structure. Expression of RSK4 is seen in various tissues including brain kidney and endothelial cells indicating its diverse functional roles across different cell types and systems.

Biological function summary

RSK4 has a role in regulating cell growth and survival. It can influence transcriptional processes and protein synthesis. Despite being not extensively documented in biological research as other family members its kinase activity suggests that it participates in various cellular processes by phosphorylating specific substrates. RSK4 operates as an individual kinase while its related family members may form complexes for more coordinated activities. Its action in cell cycle regulation highlights its importance in maintaining cellular function.

Pathways

RSK4 integrates into well-known signaling pathways including the MAPK/ERK pathway. This association implies it has a role in the regulation of gene expression mitosis and cell survival linking it with other kinases like ERK1 and ERK2. Furthermore it can interact with pathways involving the PI3K/AKT signaling cascade contributing to additional regulation of cell growth thereby influencing diverse physiological functions. The involvement of RSK4 in these pathways highlights its potential interactions with other proteins such as MEK and mTOR which are pivotal in driving intracellular responses.

RSK4 has associations with various conditions including cancer and specific genetic disorders. It has been linked with tumor suppression suggesting that dysregulation of RSK4 may contribute to oncogenic processes. Additionally alterations in RSK4 expression or activity have potential connections with mental health disorders due to its expression in the brain. It interacts biologically with proteins like p53 and other growth factor receptors which are also implicated in these disease processes. Understanding RSK4's role in these contexts can provide insights into therapeutic targets for managing such conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Constitutively active serine/threonine-protein kinase that exhibits growth-factor-independent kinase activity and that may participate in p53/TP53-dependent cell growth arrest signaling and play an inhibitory role during embryogenesis.

See full target information RPS6KA6 phospho S232

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RSK4 (phospho S232) antibody [EP1524Y] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSK4 (phospho S232) antibody [EP1524Y] - BSA and Azide free (AB284772)

Western blot - Anti-RSK4 (phospho S232) antibody [EP1524Y] - BSA and Azide free (AB284772)

ELISA - Anti-RSK4 (phospho S232) antibody [EP1524Y] - BSA and Azide free (AB284772)

Dot Blot - Anti-RSK4 (phospho S232) antibody [EP1524Y] - BSA and Azide free (AB284772)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise